Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize
Photosynthesis, which is the foundation of crop growth and development, is accompanied by complex transcriptional regulatory mechanisms. Research has established that brassinosteroids (BRs) play a role in regulating plant photosynthesis, with the majority of research focusing on the physiological le...
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MDPI AG
2025-01-01
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| Series: | Biomolecules |
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| author | Hui Li Xuewu He Huayang Lv Hongyu Zhang Fuhai Peng Jun Song Wenjuan Liu Junjie Zhang |
| author_facet | Hui Li Xuewu He Huayang Lv Hongyu Zhang Fuhai Peng Jun Song Wenjuan Liu Junjie Zhang |
| author_sort | Hui Li |
| collection | DOAJ |
| description | Photosynthesis, which is the foundation of crop growth and development, is accompanied by complex transcriptional regulatory mechanisms. Research has established that brassinosteroids (BRs) play a role in regulating plant photosynthesis, with the majority of research focusing on the physiological level and regulation of rate-limiting enzymes in the dark reactions of photosynthesis. However, studies on their effects on maize photosynthesis, specifically on light-harvesting antenna proteins, have yet to be conducted. The peripheral light-harvesting antenna protein <i>Lhcb5</i> is crucial for capturing and dissipating light energy. Herein, by analyzing the transcriptomic data of maize seedling leaves treated with 24-epibrassinolide (EBR) and verifying them using qPCR experiments, we found that the MYBR17 transcription factor may regulate the expression of the photosynthetic light-harvesting antenna protein gene. Further experiments using protoplast transient expression and yeast one-hybrid tests showed that the maize transcription factor MYBR17 responds to EBR signals and binds to the promoter of the light-harvesting antenna protein <i>Lhcb5</i>, thereby upregulating its expression. These results were validated using an Arabidopsis <i>mybr17</i> mutant. Our results offer a theoretical foundation for the application of BRs to enhance the photosynthetic efficiency of maize. |
| format | Article |
| id | doaj-art-8649f4faa8f142f99598580fe42cd346 |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-8649f4faa8f142f99598580fe42cd3462025-01-24T13:25:09ZengMDPI AGBiomolecules2218-273X2025-01-011519410.3390/biom15010094Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in MaizeHui Li0Xuewu He1Huayang Lv2Hongyu Zhang3Fuhai Peng4Jun Song5Wenjuan Liu6Junjie Zhang7Institute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625000, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625000, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625000, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625000, ChinaInstitute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 611130, ChinaInstitute of Quality Standard and Testing Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu 611130, ChinaCollege of Life Science, Sichuan Agricultural University, Ya’an 625000, ChinaPhotosynthesis, which is the foundation of crop growth and development, is accompanied by complex transcriptional regulatory mechanisms. Research has established that brassinosteroids (BRs) play a role in regulating plant photosynthesis, with the majority of research focusing on the physiological level and regulation of rate-limiting enzymes in the dark reactions of photosynthesis. However, studies on their effects on maize photosynthesis, specifically on light-harvesting antenna proteins, have yet to be conducted. The peripheral light-harvesting antenna protein <i>Lhcb5</i> is crucial for capturing and dissipating light energy. Herein, by analyzing the transcriptomic data of maize seedling leaves treated with 24-epibrassinolide (EBR) and verifying them using qPCR experiments, we found that the MYBR17 transcription factor may regulate the expression of the photosynthetic light-harvesting antenna protein gene. Further experiments using protoplast transient expression and yeast one-hybrid tests showed that the maize transcription factor MYBR17 responds to EBR signals and binds to the promoter of the light-harvesting antenna protein <i>Lhcb5</i>, thereby upregulating its expression. These results were validated using an Arabidopsis <i>mybr17</i> mutant. Our results offer a theoretical foundation for the application of BRs to enhance the photosynthetic efficiency of maize.https://www.mdpi.com/2218-273X/15/1/94photosynthesisantenna proteinsMYBR17<i>Lhcb5</i> |
| spellingShingle | Hui Li Xuewu He Huayang Lv Hongyu Zhang Fuhai Peng Jun Song Wenjuan Liu Junjie Zhang Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize Biomolecules photosynthesis antenna proteins MYBR17 <i>Lhcb5</i> |
| title | Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize |
| title_full | Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize |
| title_fullStr | Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize |
| title_full_unstemmed | Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize |
| title_short | Epibrassinolide Regulates <i>Lhcb5</i> Expression Though the Transcription Factor of MYBR17 in Maize |
| title_sort | epibrassinolide regulates i lhcb5 i expression though the transcription factor of mybr17 in maize |
| topic | photosynthesis antenna proteins MYBR17 <i>Lhcb5</i> |
| url | https://www.mdpi.com/2218-273X/15/1/94 |
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