Expression of SIRT1 and DBC1 in Developing and Adult Retinas
Sirtuin 1 (SIRT1) is a deacetylase that can regulate various biological processes via repression of transcription. Its activity has been linked to the differentiation of neural progenitor cells, although little is known about its function during retinal development. The study described herein was un...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2012-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2012/908183 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551744745242624 |
|---|---|
| author | Shawn C. Maloney Emilia Antecka Alexandre N. Odashiro Bruno F. Fernandes Madeline Doyle Li-Anne Lim Yousef Katib Miguel N. Burnier |
| author_facet | Shawn C. Maloney Emilia Antecka Alexandre N. Odashiro Bruno F. Fernandes Madeline Doyle Li-Anne Lim Yousef Katib Miguel N. Burnier |
| author_sort | Shawn C. Maloney |
| collection | DOAJ |
| description | Sirtuin 1 (SIRT1) is a deacetylase that can regulate various biological processes via repression of transcription. Its activity has been linked to the differentiation of neural progenitor cells, although little is known about its function during retinal development. The study described herein was undertaken to evaluate the expression of SIRT1 and its innate inhibitor, DBC1, in retinal tissues and progenitor cells. We found both SIRT1 and DBC1 to be widely expressed in mouse and human retinas, with subtle differences in subcellular distribution of each protein. We further demonstrate that nuclear-localized SIRT1 is only seen in human-derived retinal progenitor cells and not in adult retinas, suggesting that this nuclear localization may be important in retinal development. Moreover, we observed cytoplasmic DBC1 in a subset of progenitor cells as well as in mature ganglion cells, indicating that the progenitor cell subset, which was comprised predominantly of small cells, may represent a population of ganglion cell precursors. Collectively, the data presented in this study provide support for SIRT1 and DBC1 as regulators of retinal development and normal retinal physiology. |
| format | Article |
| id | doaj-art-86215cdb9d7a40f7bf7785feac7ac6ea |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-86215cdb9d7a40f7bf7785feac7ac6ea2025-02-03T06:00:45ZengWileyStem Cells International1687-966X1687-96782012-01-01201210.1155/2012/908183908183Expression of SIRT1 and DBC1 in Developing and Adult RetinasShawn C. Maloney0Emilia Antecka1Alexandre N. Odashiro2Bruno F. Fernandes3Madeline Doyle4Li-Anne Lim5Yousef Katib6Miguel N. Burnier7Henry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaHenry C. Witelson Ocular Pathology Laboratory, McGill University, Montreal, QC, H3A 2B4, CanadaSirtuin 1 (SIRT1) is a deacetylase that can regulate various biological processes via repression of transcription. Its activity has been linked to the differentiation of neural progenitor cells, although little is known about its function during retinal development. The study described herein was undertaken to evaluate the expression of SIRT1 and its innate inhibitor, DBC1, in retinal tissues and progenitor cells. We found both SIRT1 and DBC1 to be widely expressed in mouse and human retinas, with subtle differences in subcellular distribution of each protein. We further demonstrate that nuclear-localized SIRT1 is only seen in human-derived retinal progenitor cells and not in adult retinas, suggesting that this nuclear localization may be important in retinal development. Moreover, we observed cytoplasmic DBC1 in a subset of progenitor cells as well as in mature ganglion cells, indicating that the progenitor cell subset, which was comprised predominantly of small cells, may represent a population of ganglion cell precursors. Collectively, the data presented in this study provide support for SIRT1 and DBC1 as regulators of retinal development and normal retinal physiology.http://dx.doi.org/10.1155/2012/908183 |
| spellingShingle | Shawn C. Maloney Emilia Antecka Alexandre N. Odashiro Bruno F. Fernandes Madeline Doyle Li-Anne Lim Yousef Katib Miguel N. Burnier Expression of SIRT1 and DBC1 in Developing and Adult Retinas Stem Cells International |
| title | Expression of SIRT1 and DBC1 in Developing and Adult Retinas |
| title_full | Expression of SIRT1 and DBC1 in Developing and Adult Retinas |
| title_fullStr | Expression of SIRT1 and DBC1 in Developing and Adult Retinas |
| title_full_unstemmed | Expression of SIRT1 and DBC1 in Developing and Adult Retinas |
| title_short | Expression of SIRT1 and DBC1 in Developing and Adult Retinas |
| title_sort | expression of sirt1 and dbc1 in developing and adult retinas |
| url | http://dx.doi.org/10.1155/2012/908183 |
| work_keys_str_mv | AT shawncmaloney expressionofsirt1anddbc1indevelopingandadultretinas AT emiliaantecka expressionofsirt1anddbc1indevelopingandadultretinas AT alexandrenodashiro expressionofsirt1anddbc1indevelopingandadultretinas AT brunoffernandes expressionofsirt1anddbc1indevelopingandadultretinas AT madelinedoyle expressionofsirt1anddbc1indevelopingandadultretinas AT liannelim expressionofsirt1anddbc1indevelopingandadultretinas AT yousefkatib expressionofsirt1anddbc1indevelopingandadultretinas AT miguelnburnier expressionofsirt1anddbc1indevelopingandadultretinas |