A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand
<i>Kaempferia grandifolia</i> Saensouk & Jenjittikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of <i>K. grandifolia</i&g...
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2024-12-01
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| author | Sukanya Nonthalee Surapon Saensouk Suthira Maneechai Phetlasy Souladeth Piyaporn Saensouk |
| author_facet | Sukanya Nonthalee Surapon Saensouk Suthira Maneechai Phetlasy Souladeth Piyaporn Saensouk |
| author_sort | Sukanya Nonthalee |
| collection | DOAJ |
| description | <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of <i>K. grandifolia</i> plants to induce the development of shoots, roots, microrhizomes, and acclimatization. The genetic variations between plants grown under in vitro propagation and plants grown in nature were also assessed. An efficient protocol was developed for in vitro propagation of <i>K. grandifolia</i> using microshoots (0.5 cm in length). The optimal responses for shoot multiplication (4.78 ± 1.15 shoots/explant) and root induction (11.56 ± 1.39 roots/explant) were obtained within 8 weeks in Murashige and Skoog (MS) medium supplemented with 3 mg/L of 6-benzylamino purine (BAP) plus 0.5 mg/L of NAA. Microrhizome induction was 80% when the shoot buds were cultured on MS medium supplemented with 2 mg/L of BAP, 1 mg/L of NAA, 11 µM of silver nitrate (AgNO<sub>3</sub>), and 70 g/L of sucrose after 20 weeks. The survival rate of plantlets was 100% when transplanted to soil and sand. Micropropagated and field-grown donor plants of <i>K. grandifolia</i> showed 91.49% genetic similarity through RAPD analysis. |
| format | Article |
| id | doaj-art-860f1800b92f454992b2bf31d9d0ea4d |
| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-12-01 |
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| record_format | Article |
| series | Horticulturae |
| spelling | doaj-art-860f1800b92f454992b2bf31d9d0ea4d2025-01-24T13:34:26ZengMDPI AGHorticulturae2311-75242024-12-01111610.3390/horticulturae11010006A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in ThailandSukanya Nonthalee0Surapon Saensouk1Suthira Maneechai2Phetlasy Souladeth3Piyaporn Saensouk4Horticultural Research Institute, Trang Horticultural Research Center, Trang 92150, ThailandWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biology, Faculty of Science, Mahasarakham University, Maha Sarakham 44150, ThailandFaculty of Forest Science, National University of Laos, Vientiane 7322, LaosDiversity of Family Zingiberaceae and Vascular Plant for Its Applications Research Unit, Mahasarakham University, Maha Sarakham 44150, Thailand<i>Kaempferia grandifolia</i> Saensouk & Jenjittikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of <i>K. grandifolia</i> plants to induce the development of shoots, roots, microrhizomes, and acclimatization. The genetic variations between plants grown under in vitro propagation and plants grown in nature were also assessed. An efficient protocol was developed for in vitro propagation of <i>K. grandifolia</i> using microshoots (0.5 cm in length). The optimal responses for shoot multiplication (4.78 ± 1.15 shoots/explant) and root induction (11.56 ± 1.39 roots/explant) were obtained within 8 weeks in Murashige and Skoog (MS) medium supplemented with 3 mg/L of 6-benzylamino purine (BAP) plus 0.5 mg/L of NAA. Microrhizome induction was 80% when the shoot buds were cultured on MS medium supplemented with 2 mg/L of BAP, 1 mg/L of NAA, 11 µM of silver nitrate (AgNO<sub>3</sub>), and 70 g/L of sucrose after 20 weeks. The survival rate of plantlets was 100% when transplanted to soil and sand. Micropropagated and field-grown donor plants of <i>K. grandifolia</i> showed 91.49% genetic similarity through RAPD analysis.https://www.mdpi.com/2311-7524/11/1/6anatomy<i>Kaempferia grandifolia</i>micropropagationmicrorhizomeRAPD analysis |
| spellingShingle | Sukanya Nonthalee Surapon Saensouk Suthira Maneechai Phetlasy Souladeth Piyaporn Saensouk A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand Horticulturae anatomy <i>Kaempferia grandifolia</i> micropropagation microrhizome RAPD analysis |
| title | A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand |
| title_full | A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand |
| title_fullStr | A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand |
| title_full_unstemmed | A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand |
| title_short | A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand |
| title_sort | tissue culture technique for rapid clonal propagation microrhizome induction and rapd analysis of i kaempferia grandifolia i saensouk jenjittikul a rare plant species in thailand |
| topic | anatomy <i>Kaempferia grandifolia</i> micropropagation microrhizome RAPD analysis |
| url | https://www.mdpi.com/2311-7524/11/1/6 |
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