A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand

A Tissue Culture Technique for Rapid Clonal Propagation, Microrhizome Induction, and RAPD Analysis of <i>Kaempferia grandifolia</i> Saensouk & Jenjittikul—A Rare Plant Species in Thailand

<i>Kaempferia grandifolia</i> Saensouk & Jenjittikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of <i>K. grandifolia</i&g...

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Bibliographic Details
Main Authors: Sukanya Nonthalee, Surapon Saensouk, Suthira Maneechai, Phetlasy Souladeth, Piyaporn Saensouk
Format: Article
Language:English
Published: MDPI AG 2024-12-01
Series:Horticulturae
Subjects:
anatomy
<i>Kaempferia grandifolia</i>
micropropagation
microrhizome
RAPD analysis
Online Access:https://www.mdpi.com/2311-7524/11/1/6
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Summary:<i>Kaempferia grandifolia</i> Saensouk & Jenjittikul (Zingiberaceae), a rare plant species in Thailand, is commonly used as a medicinal plant, while the young leaves are cooked and eaten as a vegetable. This study focused on the in vitro propagation of <i>K. grandifolia</i> plants to induce the development of shoots, roots, microrhizomes, and acclimatization. The genetic variations between plants grown under in vitro propagation and plants grown in nature were also assessed. An efficient protocol was developed for in vitro propagation of <i>K. grandifolia</i> using microshoots (0.5 cm in length). The optimal responses for shoot multiplication (4.78 ± 1.15 shoots/explant) and root induction (11.56 ± 1.39 roots/explant) were obtained within 8 weeks in Murashige and Skoog (MS) medium supplemented with 3 mg/L of 6-benzylamino purine (BAP) plus 0.5 mg/L of NAA. Microrhizome induction was 80% when the shoot buds were cultured on MS medium supplemented with 2 mg/L of BAP, 1 mg/L of NAA, 11 µM of silver nitrate (AgNO<sub>3</sub>), and 70 g/L of sucrose after 20 weeks. The survival rate of plantlets was 100% when transplanted to soil and sand. Micropropagated and field-grown donor plants of <i>K. grandifolia</i> showed 91.49% genetic similarity through RAPD analysis.
ISSN:2311-7524

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