Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes
Objectives. To determine differences in AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the role of AIM2 in RA fibroblast-like synoviocytes (RA-FLS). Methods. Serum AIM2 levels among health controls (HC, n=20), OA (n=25), and RA (n =49...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2020/1693730 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832560122248822784 |
|---|---|
| author | Yong Chen Qiu Fujuan Ensheng Chen Beijia Yu Fangfang Zuo Yi Yuan Xiaofeng Zhao Changhong Xiao |
| author_facet | Yong Chen Qiu Fujuan Ensheng Chen Beijia Yu Fangfang Zuo Yi Yuan Xiaofeng Zhao Changhong Xiao |
| author_sort | Yong Chen |
| collection | DOAJ |
| description | Objectives. To determine differences in AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the role of AIM2 in RA fibroblast-like synoviocytes (RA-FLS). Methods. Serum AIM2 levels among health controls (HC, n=20), OA (n=25), and RA (n =49) patients were compared via ELISA. The different expression levels of AIM2, ASC, caspase-1, and IL-1β between RA and OA synovium were semiquantified by qRT-PCR and immunohistochemical (IHC) staining. IHC staining was recorded by H scores, and its correlation with the ESR and CRP levels of RA patients was determined. SiRNA AIM2 was transferred to RA-FLS and its effects on the proliferation and migration via CCK-8 assay and Transwell test, respectively. Results. In RA sera, the HC expressed higher level of AIM2 than OA and RA patients, and ASC, caspase-1, and IL-1β expressed higher in RA patients than HC; no significant differences were observed between sera of OA and RA patients. However, in affected knee synovium, AIM2, ASC, caspase-1, and IL-1β were expressed higher in RA than that of OA. Moreover, the H scores of AIM2, ASC, and IL-1β were positively correlated with the ESR and CRP levels in RA patients. The proliferation of FLS was significantly inhibited after transferring with AIM2 siRNA to FLS. There were no differences in apoptosis and migration assay between the si-AIM2 group and the control group. Conclusion. AIM2 inflammasome pathway involves in the pathogenesis of RA. si-AIM2 inhibits the proliferation of RA-FLS, which may be a promising therapeutic strategy for the treatment of RA. |
| format | Article |
| id | doaj-art-860a9b38f60841a891b43a939b7c9431 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-860a9b38f60841a891b43a939b7c94312025-02-03T01:28:18ZengWileyMediators of Inflammation0962-93511466-18612020-01-01202010.1155/2020/16937301693730Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like SynoviocytesYong Chen0Qiu Fujuan1Ensheng Chen2Beijia Yu3Fangfang Zuo4Yi Yuan5Xiaofeng Zhao6Changhong Xiao7Rheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaRheumatology Department, Integrated Traditional Chinese and Western Medicine Hospital, Southern Medical University, Guangzhou 510315, ChinaObjectives. To determine differences in AIM2 inflammasome expression levels between rheumatoid arthritis (RA) and osteoarthritis (OA) and to investigate the role of AIM2 in RA fibroblast-like synoviocytes (RA-FLS). Methods. Serum AIM2 levels among health controls (HC, n=20), OA (n=25), and RA (n =49) patients were compared via ELISA. The different expression levels of AIM2, ASC, caspase-1, and IL-1β between RA and OA synovium were semiquantified by qRT-PCR and immunohistochemical (IHC) staining. IHC staining was recorded by H scores, and its correlation with the ESR and CRP levels of RA patients was determined. SiRNA AIM2 was transferred to RA-FLS and its effects on the proliferation and migration via CCK-8 assay and Transwell test, respectively. Results. In RA sera, the HC expressed higher level of AIM2 than OA and RA patients, and ASC, caspase-1, and IL-1β expressed higher in RA patients than HC; no significant differences were observed between sera of OA and RA patients. However, in affected knee synovium, AIM2, ASC, caspase-1, and IL-1β were expressed higher in RA than that of OA. Moreover, the H scores of AIM2, ASC, and IL-1β were positively correlated with the ESR and CRP levels in RA patients. The proliferation of FLS was significantly inhibited after transferring with AIM2 siRNA to FLS. There were no differences in apoptosis and migration assay between the si-AIM2 group and the control group. Conclusion. AIM2 inflammasome pathway involves in the pathogenesis of RA. si-AIM2 inhibits the proliferation of RA-FLS, which may be a promising therapeutic strategy for the treatment of RA.http://dx.doi.org/10.1155/2020/1693730 |
| spellingShingle | Yong Chen Qiu Fujuan Ensheng Chen Beijia Yu Fangfang Zuo Yi Yuan Xiaofeng Zhao Changhong Xiao Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes Mediators of Inflammation |
| title | Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes |
| title_full | Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes |
| title_fullStr | Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes |
| title_full_unstemmed | Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes |
| title_short | Expression of AIM2 in Rheumatoid Arthritis and Its Role on Fibroblast-Like Synoviocytes |
| title_sort | expression of aim2 in rheumatoid arthritis and its role on fibroblast like synoviocytes |
| url | http://dx.doi.org/10.1155/2020/1693730 |
| work_keys_str_mv | AT yongchen expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT qiufujuan expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT enshengchen expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT beijiayu expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT fangfangzuo expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT yiyuan expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT xiaofengzhao expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes AT changhongxiao expressionofaim2inrheumatoidarthritisanditsroleonfibroblastlikesynoviocytes |