Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws
This study intended to characterize pharmacologically the mediator(s ) released in the inflammation induced by Soluble Egg Antigen (SEA), the main antigen released from eggs of Schistosoma mansoni, in rat hindpaws . A single intraplantar injection of 0.1–100 μg SEA at day zero induced a dose-depende...
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359890947 |
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| author | C. M. F. Pacheco C. A. P. Tavares P. M. Z. Coelho O. A. Rocha J. M. M. Santos F. R. M. Prado J. N. Francischi |
| author_facet | C. M. F. Pacheco C. A. P. Tavares P. M. Z. Coelho O. A. Rocha J. M. M. Santos F. R. M. Prado J. N. Francischi |
| author_sort | C. M. F. Pacheco |
| collection | DOAJ |
| description | This study intended to characterize pharmacologically the mediator(s ) released in the inflammation induced by Soluble Egg Antigen (SEA), the main antigen released from eggs of Schistosoma mansoni, in rat hindpaws . A single intraplantar injection of 0.1–100 μg SEA at day zero induced a dose-dependent increase in the volume of rat hindpaws characterizing an oedema of quick onset (within 15 min) and
4 h-duration, which was confirmed by his topathological analys is of the paws . A second injection of SEA
in the same paw (1–10 μg) 28 days later induced an increased dose -dependent oedematogenic response.
The early oedematogenic response following SEA sensitization was derived from serotonin release and
interleukin-1 (IL-1), since treatment with either pizotifen or anantibody against IL-1, reduced the
response by 60% and 48%, respectively. The increased oedematogenic response derived from SEA-challenge (10 μg) of rat paws derived from a local rather than systemic reaction, since it was not
observed if the sensitization was in the contralateral paw or the peritoneal cavity of the animals. Chronic
tre atment with inhibitors of IL-2 synthesis / release such as cyclosporin or dexamethasone during the
sens itization phase reduced the oedematogenic response due to SEA challenge by 51% and 55%,
respectively. These data suggested that SEA-challenge was immune-derived and dependent of IL-2 release. It
is discussed the association between cytokine release and the resistance of rats to S. mans o ni infe ction. |
| format | Article |
| id | doaj-art-85f809a6dbfe4157888c9cbf413be465 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-85f809a6dbfe4157888c9cbf413be4652025-02-03T01:09:29ZengWileyMediators of Inflammation0962-93511466-18611998-01-017426126710.1080/09629359890947Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat pawsC. M. F. Pacheco0C. A. P. Tavares1P. M. Z. Coelho2O. A. Rocha3J. M. M. Santos4F. R. M. Prado5J. N. Francischi6Department of Pharmacology, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of Biochemistry, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of Parasitology, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of General Pathology of Biological Science, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of Pharmacology, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of Pharmacology, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilDepartment of Pharmacology, Institute of Federal University of Minas Gerais, Campus da Pampulha, Avenida Antonio Carlos 6627, Belo Horizonte 31270–100, BrazilThis study intended to characterize pharmacologically the mediator(s ) released in the inflammation induced by Soluble Egg Antigen (SEA), the main antigen released from eggs of Schistosoma mansoni, in rat hindpaws . A single intraplantar injection of 0.1–100 μg SEA at day zero induced a dose-dependent increase in the volume of rat hindpaws characterizing an oedema of quick onset (within 15 min) and 4 h-duration, which was confirmed by his topathological analys is of the paws . A second injection of SEA in the same paw (1–10 μg) 28 days later induced an increased dose -dependent oedematogenic response. The early oedematogenic response following SEA sensitization was derived from serotonin release and interleukin-1 (IL-1), since treatment with either pizotifen or anantibody against IL-1, reduced the response by 60% and 48%, respectively. The increased oedematogenic response derived from SEA-challenge (10 μg) of rat paws derived from a local rather than systemic reaction, since it was not observed if the sensitization was in the contralateral paw or the peritoneal cavity of the animals. Chronic tre atment with inhibitors of IL-2 synthesis / release such as cyclosporin or dexamethasone during the sens itization phase reduced the oedematogenic response due to SEA challenge by 51% and 55%, respectively. These data suggested that SEA-challenge was immune-derived and dependent of IL-2 release. It is discussed the association between cytokine release and the resistance of rats to S. mans o ni infe ction.http://dx.doi.org/10.1080/09629359890947Schistosoma mansoni Soluble Egg Antigen (SEA)OedemaSerotoninIL-1IL-2Cytokines. |
| spellingShingle | C. M. F. Pacheco C. A. P. Tavares P. M. Z. Coelho O. A. Rocha J. M. M. Santos F. R. M. Prado J. N. Francischi Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws Mediators of Inflammation Schistosoma mansoni Soluble Egg Antigen (SEA) Oedema Serotonin IL-1 IL-2 Cytokines. |
| title | Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws |
| title_full | Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws |
| title_fullStr | Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws |
| title_full_unstemmed | Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws |
| title_short | Pharmacological evidence supporting a role for IL-1, IL-2 and serotonin in the inflammation induced by Schistosoma mansoni Soluble Egg Antigen (SEA) in rat paws |
| title_sort | pharmacological evidence supporting a role for il 1 il 2 and serotonin in the inflammation induced by schistosoma mansoni soluble egg antigen sea in rat paws |
| topic | Schistosoma mansoni Soluble Egg Antigen (SEA) Oedema Serotonin IL-1 IL-2 Cytokines. |
| url | http://dx.doi.org/10.1080/09629359890947 |
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