In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis
Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess t...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2015/489842 |
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| _version_ | 1832564620050563072 |
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| author | Arash Azizi Shabnam Aghayan Saeed Zaker Mahdieh Shakeri Navid Entezari Shirin Lawaf |
| author_facet | Arash Azizi Shabnam Aghayan Saeed Zaker Mahdieh Shakeri Navid Entezari Shirin Lawaf |
| author_sort | Arash Azizi |
| collection | DOAJ |
| description | Background and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations of Zingiber officinale extract on proliferation of Streptococcus mutans and Streptococcus sanguinis in vitro. Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛ of each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛ of each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described. Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL for S. mutans and 0.3 mg/mL for S. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg for S. mutans and 0.6 mg for S. sanguinis. Conclusion. Zingiber officinale extract has significant antibacterial activity against S. mutans and S. sanguinis cariogenic microorganisms. |
| format | Article |
| id | doaj-art-85e1b2ea2a8049769e608c5086fe4cd9 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-85e1b2ea2a8049769e608c5086fe4cd92025-02-03T01:10:38ZengWileyInternational Journal of Dentistry1687-87281687-87362015-01-01201510.1155/2015/489842489842In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinisArash Azizi0Shabnam Aghayan1Saeed Zaker2Mahdieh Shakeri3Navid Entezari4Shirin Lawaf5Oral Medicine Department, Islamic Azad University Dental Branch, Tehran, IranPeriodontics Department, Islamic Azad University Dental Branch, Tehran, IranMicrobiology Department, Islamic Azad University, Parand Branch, Tehran, IranIslamic Azad University Dental Branch, Tehran, IranIslamic Azad University Dental Branch, Tehran, IranProsthodontics Department, Islamic Azad University Dental Branch, Tehran, IranBackground and Objectives. Tooth decay is an infectious disease of microbial origin. Considering the increasing prevalence of antibiotic resistance due to their overuse and also their side effects, medicinal plants are now considered for use against bacterial infections. This study aimed to assess the effects of different concentrations of Zingiber officinale extract on proliferation of Streptococcus mutans and Streptococcus sanguinis in vitro. Materials and Methods. In this experimental study, serial dilutions of the extract were prepared in two sets of 10 test tubes for each bacterium (total of 20). Standard amounts of bacterial suspension were added; 100ƛ of each tube was cultured on prepared solid agar plates and incubated at 37°C for 24 hours. Serial dilutions of the extract were prepared in another 20 tubes and 100ƛ of each tube was added to blood agar culture medium while being prepared. The mixture was transferred to the plates. The bacteria were inoculated on plates and incubated as described. Results. The minimum inhibitory concentration (MIC) was 0.02 mg/mL for S. mutans and 0.3 mg/mL for S. sanguinis. The minimum bactericidal concentration (MBC) was 0.04 mg for S. mutans and 0.6 mg for S. sanguinis. Conclusion. Zingiber officinale extract has significant antibacterial activity against S. mutans and S. sanguinis cariogenic microorganisms.http://dx.doi.org/10.1155/2015/489842 |
| spellingShingle | Arash Azizi Shabnam Aghayan Saeed Zaker Mahdieh Shakeri Navid Entezari Shirin Lawaf In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis International Journal of Dentistry |
| title | In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis |
| title_full | In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis |
| title_fullStr | In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis |
| title_full_unstemmed | In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis |
| title_short | In Vitro Effect of Zingiber officinale Extract on Growth of Streptococcus mutans and Streptococcus sanguinis |
| title_sort | in vitro effect of zingiber officinale extract on growth of streptococcus mutans and streptococcus sanguinis |
| url | http://dx.doi.org/10.1155/2015/489842 |
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