Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease
A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2013/396156 |
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| author | Tanveer Majeed Romana Tabassum William J. Orts Charles C. Lee |
| author_facet | Tanveer Majeed Romana Tabassum William J. Orts Charles C. Lee |
| author_sort | Tanveer Majeed |
| collection | DOAJ |
| description | A putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market. |
| format | Article |
| id | doaj-art-85b9b23163c7424f939550774ab36e2a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-85b9b23163c7424f939550774ab36e2a2025-02-03T06:13:00ZengWileyThe Scientific World Journal1537-744X2013-01-01201310.1155/2013/396156396156Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine ProteaseTanveer Majeed0Romana Tabassum1William J. Orts2Charles C. Lee3National Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad 38000, PakistanNational Institute for Biotechnology and Genetic Engineering (NIBGE), P.O. Box 577, Jhang Road, Faisalabad 38000, PakistanUSDA-ARS, Bioproduct Chemistry and Engineering Research Unit, 800 Buchanan Street, Albany, CA 94710, USAUSDA-ARS, Bioproduct Chemistry and Engineering Research Unit, 800 Buchanan Street, Albany, CA 94710, USAA putative protease gene (aprE) from the thermophilic bacterium Coprothermobacter proteolyticus was cloned and expressed in Bacillus subtilis. The enzyme was determined to be a serine protease based on inhibition by PMSF. Biochemical characterization demonstrated that the enzyme had optimal activity under alkaline conditions (pH 8–10). In addition, the enzyme had an elevated optimum temperature (60°C). The protease was also stable in the presence of many surfactants and oxidant. Thus, the C. proteolyticus protease has potential applications in industries such as the detergent market.http://dx.doi.org/10.1155/2013/396156 |
| spellingShingle | Tanveer Majeed Romana Tabassum William J. Orts Charles C. Lee Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease The Scientific World Journal |
| title | Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease |
| title_full | Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease |
| title_fullStr | Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease |
| title_full_unstemmed | Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease |
| title_short | Expression and Characterization of Coprothermobacter proteolyticus Alkaline Serine Protease |
| title_sort | expression and characterization of coprothermobacter proteolyticus alkaline serine protease |
| url | http://dx.doi.org/10.1155/2013/396156 |
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