Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis.
Background: Primary myelofibrosis is a chronic myeloproliferative neoplasm characterized by abnormal megakaryocyte proliferation and fibrosis that destroys healthy bone marrow. This results in extramedullary hematopoiesis, variable blood cell deficiencies, hepatosplenomegaly, general symptoms, prog...
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College of Medicine University of Baghdad
2024-12-01
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| Series: | مجلة كلية الطب |
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| Online Access: | https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/2400 |
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| author | Sarah I. Khaleel Jaffar N. AlAlsaidissa |
| author_facet | Sarah I. Khaleel Jaffar N. AlAlsaidissa |
| author_sort | Sarah I. Khaleel |
| collection | DOAJ |
| description |
Background: Primary myelofibrosis is a chronic myeloproliferative neoplasm characterized by abnormal megakaryocyte proliferation and fibrosis that destroys healthy bone marrow. This results in extramedullary hematopoiesis, variable blood cell deficiencies, hepatosplenomegaly, general symptoms, progression to leukemia, and a reduced lifespan. Myelofibrosis can occur as a de novo myeloproliferative neoplastic disorder or evolve from other myeloproliferative neoplasms, including Polycythemia Vera or Essential Thrombocytosis. MicroRNAs (miRNAs) are short, non-protein-coding RNA molecules, typically 18–24 nucleotides in length. Dysregulation of miRNAs may contribute to the disease phenotype.
Objective: To investigate the expression level of MicroRNA-155-5p in patients with Primary Myelofibrosis compared to healthy controls and its correlation with common clinic-pathological factors.
Methods: twenty-eight patients with Primary Myelofibrosis and twenty healthy subjects were examined as controls. Expression analysis of MicroRNA-155-5p was performed using reverse transcription-quantitative polymerase chain reaction (qRT-PCR) on plasma isolated from peripheral blood.
Results: MicroRNA-155-5p expression was significantly upregulated in patients with Primary Myelofibrosis (P = 0.0001). However, no significant correlations were found between MicroRNA-155-5p and age, sex, Janus kinase 2 mutation status, or hematological parameters, including hemoglobin, white blood cell count, and platelet count.
Conclusion: MicroRNA-155-5p expression is not influenced by age, sex, Janus kinase 2 mutation status, or hematological parameters. Aberrant expression of MicroRNA-155-5p may contribute to the pathogenesis of Primary Myelofibrosis, warranting further research to understand the disease mechanisms better.
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| format | Article |
| id | doaj-art-8599e12179044665af54475dcbf79588 |
| institution | Kabale University |
| issn | 0041-9419 2410-8057 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | College of Medicine University of Baghdad |
| record_format | Article |
| series | مجلة كلية الطب |
| spelling | doaj-art-8599e12179044665af54475dcbf795882025-01-19T12:24:08ZengCollege of Medicine University of Baghdadمجلة كلية الطب0041-94192410-80572024-12-0166410.32007/jfacmedbaghdad.6642400Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis.Sarah I. Khaleel0https://orcid.org/0009-0001-7975-4096Jaffar N. AlAlsaidissa1https://orcid.org/0000-0002-8065-6725Department of Pathology and Forensic Medicine, College of Medicine University of Baghdad, Baghdad, Iraq.Department of Pathology and Forensic Medicine, College of Medicine, University of Baghdad, Baghdad, Iraq. Background: Primary myelofibrosis is a chronic myeloproliferative neoplasm characterized by abnormal megakaryocyte proliferation and fibrosis that destroys healthy bone marrow. This results in extramedullary hematopoiesis, variable blood cell deficiencies, hepatosplenomegaly, general symptoms, progression to leukemia, and a reduced lifespan. Myelofibrosis can occur as a de novo myeloproliferative neoplastic disorder or evolve from other myeloproliferative neoplasms, including Polycythemia Vera or Essential Thrombocytosis. MicroRNAs (miRNAs) are short, non-protein-coding RNA molecules, typically 18–24 nucleotides in length. Dysregulation of miRNAs may contribute to the disease phenotype. Objective: To investigate the expression level of MicroRNA-155-5p in patients with Primary Myelofibrosis compared to healthy controls and its correlation with common clinic-pathological factors. Methods: twenty-eight patients with Primary Myelofibrosis and twenty healthy subjects were examined as controls. Expression analysis of MicroRNA-155-5p was performed using reverse transcription-quantitative polymerase chain reaction (qRT-PCR) on plasma isolated from peripheral blood. Results: MicroRNA-155-5p expression was significantly upregulated in patients with Primary Myelofibrosis (P = 0.0001). However, no significant correlations were found between MicroRNA-155-5p and age, sex, Janus kinase 2 mutation status, or hematological parameters, including hemoglobin, white blood cell count, and platelet count. Conclusion: MicroRNA-155-5p expression is not influenced by age, sex, Janus kinase 2 mutation status, or hematological parameters. Aberrant expression of MicroRNA-155-5p may contribute to the pathogenesis of Primary Myelofibrosis, warranting further research to understand the disease mechanisms better. https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/2400Essential thrombocytosisMicroRNAMyeloproliferative neoplasmsPolycythemia VeraPrimary Myelofibrosis |
| spellingShingle | Sarah I. Khaleel Jaffar N. AlAlsaidissa Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. مجلة كلية الطب Essential thrombocytosis MicroRNA Myeloproliferative neoplasms Polycythemia Vera Primary Myelofibrosis |
| title | Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. |
| title_full | Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. |
| title_fullStr | Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. |
| title_full_unstemmed | Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. |
| title_short | Analysis of MicroRNA -155-5p Expression in Patients with Primary Myelofibrosis. |
| title_sort | analysis of microrna 155 5p expression in patients with primary myelofibrosis |
| topic | Essential thrombocytosis MicroRNA Myeloproliferative neoplasms Polycythemia Vera Primary Myelofibrosis |
| url | https://iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/2400 |
| work_keys_str_mv | AT sarahikhaleel analysisofmicrorna1555pexpressioninpatientswithprimarymyelofibrosis AT jaffarnalalsaidissa analysisofmicrorna1555pexpressioninpatientswithprimarymyelofibrosis |