A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations]
VAPB is an adaptor protein known for its role as an anchor for other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and the underlying mechanisms resulting from this defect are studied by researchers in this area to uncover its implicati...
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2024-12-01
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| Online Access: | https://f1000research.com/articles/13-1559/v1 |
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| author | Maryam Fothouhi Peter S. McPherson Carl Laflamme Sara González Bolívar Riham Ayoubi Charles Alende Irina Shlaifer |
| author_facet | Maryam Fothouhi Peter S. McPherson Carl Laflamme Sara González Bolívar Riham Ayoubi Charles Alende Irina Shlaifer |
| author_sort | Maryam Fothouhi |
| collection | DOAJ |
| description | VAPB is an adaptor protein known for its role as an anchor for other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and the underlying mechanisms resulting from this defect are studied by researchers in this area to uncover its implication in the disease. Here we have characterized six VAPB commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs. |
| format | Article |
| id | doaj-art-85966d9c5255413a9a8cd00786635691 |
| institution | Kabale University |
| issn | 2046-1402 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | F1000 Research Ltd |
| record_format | Article |
| series | F1000Research |
| spelling | doaj-art-85966d9c5255413a9a8cd007866356912025-02-06T01:00:02ZengF1000 Research LtdF1000Research2046-14022024-12-0113176085A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations]Maryam Fothouhi0Peter S. McPherson1Carl Laflamme2https://orcid.org/0000-0001-5906-025XSara González Bolívar3https://orcid.org/0000-0002-4299-8281Riham Ayoubi4Charles Alende5https://orcid.org/0009-0005-4611-6134Irina Shlaifer6Montreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaMontreal Neurological Institute-Hospital, Montreal, Québec, CanadaVAPB is an adaptor protein known for its role as an anchor for other proteins at the endoplasmic reticulum. A mutant form of VAPB has been linked to amyotrophic lateral sclerosis and the underlying mechanisms resulting from this defect are studied by researchers in this area to uncover its implication in the disease. Here we have characterized six VAPB commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.https://f1000research.com/articles/13-1559/v1O95292 VAPB Vesicle-associated membrane protein-associated protein B/C VAMP-associated protein B/C antibody characterization antibody validationeng |
| spellingShingle | Maryam Fothouhi Peter S. McPherson Carl Laflamme Sara González Bolívar Riham Ayoubi Charles Alende Irina Shlaifer A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] F1000Research O95292 VAPB Vesicle-associated membrane protein-associated protein B/C VAMP-associated protein B/C antibody characterization antibody validation eng |
| title | A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] |
| title_full | A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] |
| title_fullStr | A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] |
| title_full_unstemmed | A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] |
| title_short | A guide to selecting high-performing antibodies for VAPB (UniProt ID: O95292) for use in western blot, immunoprecipitation, and immunofluorescence [version 1; peer review: 3 approved, 1 approved with reservations] |
| title_sort | guide to selecting high performing antibodies for vapb uniprot id o95292 for use in western blot immunoprecipitation and immunofluorescence version 1 peer review 3 approved 1 approved with reservations |
| topic | O95292 VAPB Vesicle-associated membrane protein-associated protein B/C VAMP-associated protein B/C antibody characterization antibody validation eng |
| url | https://f1000research.com/articles/13-1559/v1 |
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