Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits
Passiflora edulis Flavicarpa, Passiflora edulis Sims, and Passiflora ligularis Juss are Colombian fruits (passion fruits) of important exportation value. They act efficiently as antioxidants, antifungal, and antimicrobial compounds due to their high polyphenol content. Polyphenols can be quantified...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | International Journal of Food Science |
| Online Access: | http://dx.doi.org/10.1155/2021/8871301 |
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| author | Juan C. Carmona-Hernandez Gonzalo Taborda-Ocampo Clara H. González-Correa |
| author_facet | Juan C. Carmona-Hernandez Gonzalo Taborda-Ocampo Clara H. González-Correa |
| author_sort | Juan C. Carmona-Hernandez |
| collection | DOAJ |
| description | Passiflora edulis Flavicarpa, Passiflora edulis Sims, and Passiflora ligularis Juss are Colombian fruits (passion fruits) of important exportation value. They act efficiently as antioxidants, antifungal, and antimicrobial compounds due to their high polyphenol content. Polyphenols can be quantified by the Folin-Ciocalteu (F-C) reaction. Food matrices, solvent polarity, and several different reacting conditions are critical for the optimum extraction and quantification of polyphenols. Chromatographic identification and quantitation are satisfactory with access to a vast number of reference standards considering the availability of abundant phenolic compounds in crude extracts. The purpose of this study was to evaluate alternatives and specific F-C reacting conditions aiming at determining the highest total phenolic content (TPC) in three Colombian Passifloras. Among optimum reacting conditions, reduced reaction time and diluted alkali conditions yielded desirable positive results highlighting lower working time and minimum reagent waste production. For higher extraction yield, acetone 70% was the best solvent to capture more phenolics from the seedless pulp of these Colombian passion fruits. |
| format | Article |
| id | doaj-art-858db717fdf84c2cb83541e061744b9f |
| institution | Kabale University |
| issn | 2356-7015 2314-5765 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Food Science |
| spelling | doaj-art-858db717fdf84c2cb83541e061744b9f2025-02-03T01:24:12ZengWileyInternational Journal of Food Science2356-70152314-57652021-01-01202110.1155/2021/88713018871301Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion FruitsJuan C. Carmona-Hernandez0Gonzalo Taborda-Ocampo1Clara H. González-Correa2Grupo Investigación Médica, Universidad de Manizales, Manizales 170001, ColombiaGrupo de Investigación en Cromatografía y Técnicas Afines (GICTA), Universidad de Caldas, Manizales 170004, ColombiaGrupo Investigación Nutrición, Metabolismo y Seguridad Alimentaria (NUTRIMESA), Universidad de Caldas, Manizales 170004, ColombiaPassiflora edulis Flavicarpa, Passiflora edulis Sims, and Passiflora ligularis Juss are Colombian fruits (passion fruits) of important exportation value. They act efficiently as antioxidants, antifungal, and antimicrobial compounds due to their high polyphenol content. Polyphenols can be quantified by the Folin-Ciocalteu (F-C) reaction. Food matrices, solvent polarity, and several different reacting conditions are critical for the optimum extraction and quantification of polyphenols. Chromatographic identification and quantitation are satisfactory with access to a vast number of reference standards considering the availability of abundant phenolic compounds in crude extracts. The purpose of this study was to evaluate alternatives and specific F-C reacting conditions aiming at determining the highest total phenolic content (TPC) in three Colombian Passifloras. Among optimum reacting conditions, reduced reaction time and diluted alkali conditions yielded desirable positive results highlighting lower working time and minimum reagent waste production. For higher extraction yield, acetone 70% was the best solvent to capture more phenolics from the seedless pulp of these Colombian passion fruits.http://dx.doi.org/10.1155/2021/8871301 |
| spellingShingle | Juan C. Carmona-Hernandez Gonzalo Taborda-Ocampo Clara H. González-Correa Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits International Journal of Food Science |
| title | Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits |
| title_full | Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits |
| title_fullStr | Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits |
| title_full_unstemmed | Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits |
| title_short | Folin-Ciocalteu Reaction Alternatives for Higher Polyphenol Quantitation in Colombian Passion Fruits |
| title_sort | folin ciocalteu reaction alternatives for higher polyphenol quantitation in colombian passion fruits |
| url | http://dx.doi.org/10.1155/2021/8871301 |
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