Five steps in leukocyte extravasation in the microcirculation by chemoattractants
For in vivo study of the phenomena observed in vitro, PMN (polymorphonuclear leukocyte) extravasation was analysed quantitatively in the microcirculation of the hamster cheek pouch using a video system. Topical application of leukotriene B4 or N-formyl-methionylleucyl- phenylalanine increased dose d...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1992-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935192000619 |
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| author | T. Oda M. Katori K. Hatanaka S. Yamashina |
| author_facet | T. Oda M. Katori K. Hatanaka S. Yamashina |
| author_sort | T. Oda |
| collection | DOAJ |
| description | For in vivo study of the phenomena observed in vitro, PMN (polymorphonuclear leukocyte) extravasation was analysed quantitatively in the microcirculation of the hamster cheek pouch using a video system. Topical application of leukotriene B4 or N-formyl-methionylleucyl- phenylalanine increased dose dependently the number of PMNs adhering to the venules. Eighty to 90% of the adhering PMNs disappeared from the vascular lumen into the venular wall within 10-12 rain after the adhesion. After PMNs had passed through the endothelial cell layer, they remained in the venular wall for more than 30 min after application of the chemoattractants and appeared in the extravascular space. Thus, the process could be divided into five steps: (1) rolling and (2) adhesion to the endothelium, (3) passage through the endothelial layer (4) remaining in the venular wall, and (5) passage through the basement membrane. |
| format | Article |
| id | doaj-art-8571cc4cf00840c6acb32027d6a06800 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1992-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-8571cc4cf00840c6acb32027d6a068002025-02-03T01:22:18ZengWileyMediators of Inflammation0962-93511466-18611992-01-011640340910.1155/S0962935192000619Five steps in leukocyte extravasation in the microcirculation by chemoattractantsT. Oda0M. Katori1K. Hatanaka2S. Yamashina3Department of Pharmacology, Kitasato University School of Medicine, Kanagawa, Sagamihara 228, JapanDepartment of Pharmacology, Kitasato University School of Medicine, Kanagawa, Sagamihara 228, JapanDepartment of Pharmacology, Kitasato University School of Medicine, Kanagawa, Sagamihara 228, JapanDepartment of Anatomy, Kitasato University School of Medicine, Kanagawa, Sagamihara 228, JapanFor in vivo study of the phenomena observed in vitro, PMN (polymorphonuclear leukocyte) extravasation was analysed quantitatively in the microcirculation of the hamster cheek pouch using a video system. Topical application of leukotriene B4 or N-formyl-methionylleucyl- phenylalanine increased dose dependently the number of PMNs adhering to the venules. Eighty to 90% of the adhering PMNs disappeared from the vascular lumen into the venular wall within 10-12 rain after the adhesion. After PMNs had passed through the endothelial cell layer, they remained in the venular wall for more than 30 min after application of the chemoattractants and appeared in the extravascular space. Thus, the process could be divided into five steps: (1) rolling and (2) adhesion to the endothelium, (3) passage through the endothelial layer (4) remaining in the venular wall, and (5) passage through the basement membrane.http://dx.doi.org/10.1155/S0962935192000619 |
| spellingShingle | T. Oda M. Katori K. Hatanaka S. Yamashina Five steps in leukocyte extravasation in the microcirculation by chemoattractants Mediators of Inflammation |
| title | Five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| title_full | Five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| title_fullStr | Five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| title_full_unstemmed | Five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| title_short | Five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| title_sort | five steps in leukocyte extravasation in the microcirculation by chemoattractants |
| url | http://dx.doi.org/10.1155/S0962935192000619 |
| work_keys_str_mv | AT toda fivestepsinleukocyteextravasationinthemicrocirculationbychemoattractants AT mkatori fivestepsinleukocyteextravasationinthemicrocirculationbychemoattractants AT khatanaka fivestepsinleukocyteextravasationinthemicrocirculationbychemoattractants AT syamashina fivestepsinleukocyteextravasationinthemicrocirculationbychemoattractants |