Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus

Atotal of 75 different clinical samples were collected from different hospitals in Baghdad Biochemical and morphological characterization tests showed that forty isolates were identified as Staphylococcus aureus Antibiotic susceptibility tests of all isolates towards ten antibiotics were carried ou...

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Main Author: Baghdad Science Journal
Format: Article
Language:English
Published: University of Baghdad, College of Science for Women 2009-12-01
Series:مجلة بغداد للعلوم
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Online Access:http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1032
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author Baghdad Science Journal
author_facet Baghdad Science Journal
author_sort Baghdad Science Journal
collection DOAJ
description Atotal of 75 different clinical samples were collected from different hospitals in Baghdad Biochemical and morphological characterization tests showed that forty isolates were identified as Staphylococcus aureus Antibiotic susceptibility tests of all isolates towards ten antibiotics were carried out and results showed that many isolates (97.5 %) were resistant to ?-lactam antibiotic , 70 % were resistant to Tetracyclinee , 62.5% were resistant to co-trimoxazole , 60 % were resistant to ciprofloxacin , 55% were resistant both of chloramphenicol and erythromycin , 52.5% were resistant to gentamicin , 35% were resistant to rifampicin , 10% were resistant to vancomycin . According to the above results the S.aureus I1 which is isolated from patients with osteomyelitis showed resistant to all ten antibiotics therefore was used in the followed experiments. The minimum inhibitory concentration (MIC) of S.aureus I1 vancomycin, cefotaxim , penicillin G, amoxicillin , ciprofloxacin , co-trimoxazole ,gentamicin, rifampicin was checked.The results showed that isolates had MIC between (390-12500) ?g/ml. The combination of different antibiotics with vancomycin showed synergistic effect based on the Fractional inhibitory concentration index (FIC).
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publishDate 2009-12-01
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series مجلة بغداد للعلوم
spelling doaj-art-85343bb392ca4da3afe25636aaac33462025-08-20T03:33:56ZengUniversity of Baghdad, College of Science for Womenمجلة بغداد للعلوم2078-86652411-79862009-12-016410.21123/bsj.6.4.683-692Effect the combination of antibiotics on clinical isolates of Staphylococcus aureusBaghdad Science JournalAtotal of 75 different clinical samples were collected from different hospitals in Baghdad Biochemical and morphological characterization tests showed that forty isolates were identified as Staphylococcus aureus Antibiotic susceptibility tests of all isolates towards ten antibiotics were carried out and results showed that many isolates (97.5 %) were resistant to ?-lactam antibiotic , 70 % were resistant to Tetracyclinee , 62.5% were resistant to co-trimoxazole , 60 % were resistant to ciprofloxacin , 55% were resistant both of chloramphenicol and erythromycin , 52.5% were resistant to gentamicin , 35% were resistant to rifampicin , 10% were resistant to vancomycin . According to the above results the S.aureus I1 which is isolated from patients with osteomyelitis showed resistant to all ten antibiotics therefore was used in the followed experiments. The minimum inhibitory concentration (MIC) of S.aureus I1 vancomycin, cefotaxim , penicillin G, amoxicillin , ciprofloxacin , co-trimoxazole ,gentamicin, rifampicin was checked.The results showed that isolates had MIC between (390-12500) ?g/ml. The combination of different antibiotics with vancomycin showed synergistic effect based on the Fractional inhibitory concentration index (FIC).http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1032"Staphylococcus aureus, Antibiotic susceptibility and MIC tests, combination of antibiotics"
spellingShingle Baghdad Science Journal
Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
مجلة بغداد للعلوم
"Staphylococcus aureus, Antibiotic susceptibility and MIC tests, combination of antibiotics"
title Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
title_full Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
title_fullStr Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
title_full_unstemmed Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
title_short Effect the combination of antibiotics on clinical isolates of Staphylococcus aureus
title_sort effect the combination of antibiotics on clinical isolates of staphylococcus aureus
topic "Staphylococcus aureus, Antibiotic susceptibility and MIC tests, combination of antibiotics"
url http://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/1032
work_keys_str_mv AT baghdadsciencejournal effectthecombinationofantibioticsonclinicalisolatesofstaphylococcusaureus