Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism
The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP+ (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP+ (250 μmol/L), Z-YVAD-CHO...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Behavioural Neurology |
| Online Access: | http://dx.doi.org/10.1155/2015/725897 |
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| author | Jian-xin Xu Hai-ping Song Qing-Xia Bu De-Peng Feng Xiao-Fan Xu Qian-Ru Sun Xue-Li Li |
| author_facet | Jian-xin Xu Hai-ping Song Qing-Xia Bu De-Peng Feng Xiao-Fan Xu Qian-Ru Sun Xue-Li Li |
| author_sort | Jian-xin Xu |
| collection | DOAJ |
| description | The study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP+ (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP+ (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP+ group, and Z-DEVD-CHO (10 nM) + MPP+ group. Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry. The results showed that cell viability in the MPP+ group was lower than in all other five groups. There was no difference in cell viability between isoflavone + MPP+ and control group. Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP+, and MPP+ only groups. The apoptosis ratio in the isoflavone + MPP+ group and control group and the Z-YVAD-CHO + MPP+ and Z-DEVD-CHO + MPP+ group was similar, which was lower than in the MPP+ group. The lowest apoptosis ratio was found in the isoflavone only group. |
| format | Article |
| id | doaj-art-852f7f0e14ef4d5b85ac0afcb07b2cb1 |
| institution | Kabale University |
| issn | 0953-4180 1875-8584 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Behavioural Neurology |
| spelling | doaj-art-852f7f0e14ef4d5b85ac0afcb07b2cb12025-02-03T01:31:17ZengWileyBehavioural Neurology0953-41801875-85842015-01-01201510.1155/2015/725897725897Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of ParkinsonismJian-xin Xu0Hai-ping Song1Qing-Xia Bu2De-Peng Feng3Xiao-Fan Xu4Qian-Ru Sun5Xue-Li Li6Department of Neurology, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaDepartment of General Surgery, The Affiliated Union Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, ChinaDepartment of Neurology, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaDepartment of Neurology, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaDepartment of Neurology, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaDepartment of Neuroimmune Laboratory, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaDepartment of Neurology, Liaocheng People’s Hospital and Liaocheng Clinical School of Taishan Medical University, Liaocheng, Shandong 252000, ChinaThe study has investigated the effect of isoflavone attenuates the caspase-1 and caspase-3 level in cell model of Parkinsonism. The subjects were PC12 cells. They were randomly divided into six groups: control, MPP+ (250 μmol/L), isoflavone (10 μM), isoflavone (10 μM) + MPP+ (250 μmol/L), Z-YVAD-CHO (10 nM) + MPP+ group, and Z-DEVD-CHO (10 nM) + MPP+ group. Cell viability was measured by MTT methods; the content of tyrosine hydroxylase was measured by immunocytochemistry method of avidinbiotin peroxidase complex; apoptosis ratio was measured by flow cytometry. The results showed that cell viability in the MPP+ group was lower than in all other five groups. There was no difference in cell viability between isoflavone + MPP+ and control group. Optical density of TH positive cells in isoflavone group was higher than in control, isoflavone + MPP+, and MPP+ only groups. The apoptosis ratio in the isoflavone + MPP+ group and control group and the Z-YVAD-CHO + MPP+ and Z-DEVD-CHO + MPP+ group was similar, which was lower than in the MPP+ group. The lowest apoptosis ratio was found in the isoflavone only group.http://dx.doi.org/10.1155/2015/725897 |
| spellingShingle | Jian-xin Xu Hai-ping Song Qing-Xia Bu De-Peng Feng Xiao-Fan Xu Qian-Ru Sun Xue-Li Li Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism Behavioural Neurology |
| title | Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism |
| title_full | Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism |
| title_fullStr | Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism |
| title_full_unstemmed | Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism |
| title_short | Isoflavone Attenuates the Caspase-1 and Caspase-3 Level in Cell Model of Parkinsonism |
| title_sort | isoflavone attenuates the caspase 1 and caspase 3 level in cell model of parkinsonism |
| url | http://dx.doi.org/10.1155/2015/725897 |
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