The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis
Hashimoto’s thyroiditis (HT) is an organ-specific autoimmune disorder characterized by progressive thyroid failure. Th1 and Treg subset of CD4+ cells have been implicated in the pathogenesis; however, less is known about their respective roles across the spectrum of HT clinical presentations. To she...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/3687420 |
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| author | Stana Tokić Mario Štefanić Ljubica Glavaš-Obrovac Sonja Jaman Eva Novosadová Jana Petrkova Zdenka Navratilova Mirjana Suver Stević Martin Petrek |
| author_facet | Stana Tokić Mario Štefanić Ljubica Glavaš-Obrovac Sonja Jaman Eva Novosadová Jana Petrkova Zdenka Navratilova Mirjana Suver Stević Martin Petrek |
| author_sort | Stana Tokić |
| collection | DOAJ |
| description | Hashimoto’s thyroiditis (HT) is an organ-specific autoimmune disorder characterized by progressive thyroid failure. Th1 and Treg subset of CD4+ cells have been implicated in the pathogenesis; however, less is known about their respective roles across the spectrum of HT clinical presentations. To shed more light on CD4+ subsets role in HT, we investigated the mRNA expression levels of several Th1/Treg-associated transcription factors (T-bet/ETS1, HIF1α/BLIMP1/FOXP3) in peripheral blood T cells of 10 hypothyroid, untreated HT patients, 10 hypothyroid patients undergoing hormone replacement therapy, 12 euthyroid HT subjects, and 11 healthy controls by the qRT-PCR. Compared to euthyroid HT patients and controls, both hypothyroid (2.34-fold difference versus controls, P<0.01) and thyroxine-supplemented patients (2.5-fold, P<0.001) showed an increased FOXP3 mRNA expression in T cells. Similarly, mRNA expression levels of T-bet were upregulated in severely affected but not in euthyroid HT subjects (2.37-fold and 3.2-fold, hypothyroid and thyroxine-supplemented HT patients versus controls, resp., P<0.01). By contrast, no differences in mRNA expression levels of ETS1, BLIMP1, and HIF1α were observed across the study groups. In summary, severe but not euthyroid HT was associated with robust upregulation of T-bet and FOXP3 mRNA in peripheral T cells, independent of the thyroid hormone status but proportional to disease activity. |
| format | Article |
| id | doaj-art-85269d5b45d64311b064d1b20d2b3daf |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-85269d5b45d64311b064d1b20d2b3daf2025-02-03T01:07:45ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/36874203687420The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s ThyroiditisStana Tokić0Mario Štefanić1Ljubica Glavaš-Obrovac2Sonja Jaman3Eva Novosadová4Jana Petrkova5Zdenka Navratilova6Mirjana Suver Stević7Martin Petrek8Department of Molecular Diagnostics and Tissue Typing, Osijek University Hospital, Josipa Huttlera 4, 31000 Osijek, CroatiaFaculty of Medicine, University of Osijek, Cara Hadrijana 10E, 31000 Osijek, CroatiaFaculty of Medicine, University of Osijek, Cara Hadrijana 10E, 31000 Osijek, CroatiaDepartment of Molecular Diagnostics and Tissue Typing, Osijek University Hospital, Josipa Huttlera 4, 31000 Osijek, CroatiaLaboratory of Immunogenomics, Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacký University, 775 20 Olomouc, Czech RepublicLaboratory of Immunogenomics, Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacký University, 775 20 Olomouc, Czech RepublicLaboratory of Immunogenomics, Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacký University, 775 20 Olomouc, Czech RepublicDepartment of Molecular Diagnostics and Tissue Typing, Osijek University Hospital, Josipa Huttlera 4, 31000 Osijek, CroatiaLaboratory of Immunogenomics, Department of Pathological Physiology, Faculty of Medicine and Dentistry, Palacký University, 775 20 Olomouc, Czech RepublicHashimoto’s thyroiditis (HT) is an organ-specific autoimmune disorder characterized by progressive thyroid failure. Th1 and Treg subset of CD4+ cells have been implicated in the pathogenesis; however, less is known about their respective roles across the spectrum of HT clinical presentations. To shed more light on CD4+ subsets role in HT, we investigated the mRNA expression levels of several Th1/Treg-associated transcription factors (T-bet/ETS1, HIF1α/BLIMP1/FOXP3) in peripheral blood T cells of 10 hypothyroid, untreated HT patients, 10 hypothyroid patients undergoing hormone replacement therapy, 12 euthyroid HT subjects, and 11 healthy controls by the qRT-PCR. Compared to euthyroid HT patients and controls, both hypothyroid (2.34-fold difference versus controls, P<0.01) and thyroxine-supplemented patients (2.5-fold, P<0.001) showed an increased FOXP3 mRNA expression in T cells. Similarly, mRNA expression levels of T-bet were upregulated in severely affected but not in euthyroid HT subjects (2.37-fold and 3.2-fold, hypothyroid and thyroxine-supplemented HT patients versus controls, resp., P<0.01). By contrast, no differences in mRNA expression levels of ETS1, BLIMP1, and HIF1α were observed across the study groups. In summary, severe but not euthyroid HT was associated with robust upregulation of T-bet and FOXP3 mRNA in peripheral T cells, independent of the thyroid hormone status but proportional to disease activity.http://dx.doi.org/10.1155/2016/3687420 |
| spellingShingle | Stana Tokić Mario Štefanić Ljubica Glavaš-Obrovac Sonja Jaman Eva Novosadová Jana Petrkova Zdenka Navratilova Mirjana Suver Stević Martin Petrek The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis Mediators of Inflammation |
| title | The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis |
| title_full | The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis |
| title_fullStr | The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis |
| title_full_unstemmed | The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis |
| title_short | The Expression of T Cell FOXP3 and T-Bet Is Upregulated in Severe but Not Euthyroid Hashimoto’s Thyroiditis |
| title_sort | expression of t cell foxp3 and t bet is upregulated in severe but not euthyroid hashimoto s thyroiditis |
| url | http://dx.doi.org/10.1155/2016/3687420 |
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