Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro
The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells. In this study, SD rats were divided into the normal chow diet group (NCD), high fat diet group (HFD), three treatment groups feeding high-fat diet...
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Tsinghua University Press
2023-07-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213453022002622 |
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| author | Xiaoyu Cai Songxue Wang Huali Wang Suwen Liu Guishan Liu Huibin Chen Ji Kang Hao Wang |
| author_facet | Xiaoyu Cai Songxue Wang Huali Wang Suwen Liu Guishan Liu Huibin Chen Ji Kang Hao Wang |
| author_sort | Xiaoyu Cai |
| collection | DOAJ |
| description | The aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells. In this study, SD rats were divided into the normal chow diet group (NCD), high fat diet group (HFD), three treatment groups feeding high-fat diet with naringenin (100, 200, 400 mg/kg) for 12 weeks. Results indicated that naringenin treatment decreased total cholesterol (TC), triglyceride (TG) and the non-high-density lipoprotein cholesterol (non-HDL-C) levels in serum. Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats. In addition, naringenin (25−75 µg/mL) decrease TG and TC levels in 3T3 mature adipocytes. The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology. Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes. Meanwhile, naringenin increased the AMPK (AMP-activated protein kinase) activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes. And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C. Molecular docking results indicated that naringenin could bind to AMPK protein. These results indicated naringenin reduced lipid accumulation through AMPK pathway. |
| format | Article |
| id | doaj-art-8521bd982e8d4e7fb6a375d93daca3dc |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-07-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-8521bd982e8d4e7fb6a375d93daca3dc2025-02-03T11:09:30ZengTsinghua University PressFood Science and Human Wellness2213-45302023-07-0112411741183Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitroXiaoyu Cai0Songxue Wang1Huali Wang2Suwen Liu3Guishan Liu4Huibin Chen5Ji Kang6Hao Wang7State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, ChinaChina National Center for Food Safety Risk Assessment, Beijing 100022, ChinaCollege of Food Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, ChinaSchool of Food and Wine, Ningxia University, Yinchuan 750021, ChinaCollege of Life Sciences, Fujian Normal University, Fuzhou 350117, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; Correspondence authors at: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), No. 29, 13th Avenue, Economic and Technological Development Area (TEDA), Tianjin, ChinaState Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; Correspondence authors at: State Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology (TUST), No. 29, 13th Avenue, Economic and Technological Development Area (TEDA), Tianjin, ChinaThe aim of this study was to explore the lipid-lowering effect of naringenin and the underlying mechanism in high-fat-diet-fed SD rats and 3T3-L1 cells. In this study, SD rats were divided into the normal chow diet group (NCD), high fat diet group (HFD), three treatment groups feeding high-fat diet with naringenin (100, 200, 400 mg/kg) for 12 weeks. Results indicated that naringenin treatment decreased total cholesterol (TC), triglyceride (TG) and the non-high-density lipoprotein cholesterol (non-HDL-C) levels in serum. Naringenin also alleviated hepatic steatosis and reduced the adipocyte size in the epididymis in high-fat-diet-induced SD rats. In addition, naringenin (25−75 µg/mL) decrease TG and TC levels in 3T3 mature adipocytes. The molecular mechanism of naringenin in the treatment of obesity were predicted by using network pharmacology. Real-time PCR analysis results showed that naringenin regulated the expression of lipid metabolism genes. Meanwhile, naringenin increased the AMPK (AMP-activated protein kinase) activity and the expression of AMPK phosphorylated protein in 3T3 mature adipocytes. And the inhibitory effect of naringenin on lipid accumulation in 3T3 adipocytes was abolished by Compound C. Molecular docking results indicated that naringenin could bind to AMPK protein. These results indicated naringenin reduced lipid accumulation through AMPK pathway.http://www.sciencedirect.com/science/article/pii/S2213453022002622NaringeninLipid accumulationMolecular docking |
| spellingShingle | Xiaoyu Cai Songxue Wang Huali Wang Suwen Liu Guishan Liu Huibin Chen Ji Kang Hao Wang Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro Food Science and Human Wellness Naringenin Lipid accumulation Molecular docking |
| title | Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro |
| title_full | Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro |
| title_fullStr | Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro |
| title_full_unstemmed | Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro |
| title_short | Naringenin inhibits lipid accumulation by activating the AMPK pathway in vivo and vitro |
| title_sort | naringenin inhibits lipid accumulation by activating the ampk pathway in vivo and vitro |
| topic | Naringenin Lipid accumulation Molecular docking |
| url | http://www.sciencedirect.com/science/article/pii/S2213453022002622 |
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