Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices
In this study, we present a novel approach using amperometric microsensors to detect quercetin in cosmetic formulations and track its metabolic behavior after topical application. This method offers a sensitive, real-time alternative to conventional techniques, enabling the detection of quercetin’s...
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2024-12-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/15/1/6 |
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| author | Elena Oancea Ioana Adina Tula Gabriela Stanciu Raluca-Ioana Ștefan-van Staden Jacobus (Koos) Frederick van Staden Magdalena Mititelu |
| author_facet | Elena Oancea Ioana Adina Tula Gabriela Stanciu Raluca-Ioana Ștefan-van Staden Jacobus (Koos) Frederick van Staden Magdalena Mititelu |
| author_sort | Elena Oancea |
| collection | DOAJ |
| description | In this study, we present a novel approach using amperometric microsensors to detect quercetin in cosmetic formulations and track its metabolic behavior after topical application. This method offers a sensitive, real-time alternative to conventional techniques, enabling the detection of quercetin’s bioavailability, its transformation into active metabolites, and its potential therapeutic effects when applied to the skin. Quercetin (Q) is a bioactive flavonoid known for its potent antioxidant properties, naturally present in numerous plants, particularly those with applications in cosmetic formulations. In response to the growing interest in developing novel plant-based dermo-cosmetic solutions, this study investigates the electrochemical detection of quercetin, a ketone-type flavonoid, extracted from Gingko biloba essential oil. Three newly designed amperometric microsensors were developed to assess their efficacy in detecting quercetin in botanical samples. The sensor configurations utilized two forms of carbon material as a foundation: graphite (G) and carbon nanoparticles (CNs). These base materials were modified with paraffin oil, chitosan (CHIT), and cobalt(II) tetraphenylporphyrin (Co(II)TPP) to enhance sensitivity. Differential pulse voltammetry (DPV) served as the analytical method for this investigation. Among the sensors, the CHIT/G–CN microsensor exhibited the highest sensitivity, with a detection limit of 1.22 × 10<sup>−7</sup> mol L<sup>−1</sup>, followed by the G–CN (5.64 × 10<sup>−8</sup> mol L<sup>−1</sup>) and Co(II)TPP/G–CN (9.80 × 10<sup>−8</sup> mol L<sup>−1</sup>) microsensors. The minimum detectable concentration was observed with the G–CN and CoP/G–CN microsensors, achieving a threshold as low as 0.0001 μmol L<sup>−1</sup>. Recovery rates and relative standard deviation (RSD) values averaged 97.4% ± 0.43, underscoring the sensors’ reliability for quercetin detection in botanical matrices. |
| format | Article |
| id | doaj-art-8502b3172ca34e129053f90c401516c2 |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Metabolites |
| spelling | doaj-art-8502b3172ca34e129053f90c401516c22025-01-24T13:41:08ZengMDPI AGMetabolites2218-19892024-12-01151610.3390/metabo15010006Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming PracticesElena Oancea0Ioana Adina Tula1Gabriela Stanciu2Raluca-Ioana Ștefan-van Staden3Jacobus (Koos) Frederick van Staden4Magdalena Mititelu5Laboratory of Electrochemistry and Condensed Matter, National Institute of Research for Electrochemistry and Condensed Matter, 202, Splaiul Independentei Street, 060021 Bucharest, RomaniaLaboratory of Electrochemistry and Condensed Matter, National Institute of Research for Electrochemistry and Condensed Matter, 202, Splaiul Independentei Street, 060021 Bucharest, RomaniaDepartment of Chemistry and Chemical Engineering, Ovidius University of Constanta, 900527 Constanta, RomaniaLaboratory of Electrochemistry and PATLAB, National University of Science and Technology Politehnica of Bucharest, 060042 Bucharest, RomaniaLaboratory of Electrochemistry and Condensed Matter, National Institute of Research for Electrochemistry and Condensed Matter, 202, Splaiul Independentei Street, 060021 Bucharest, RomaniaDepartment of Clinical Laboratory and Food Safety, Faculty of Pharmacy, “Carol Davila” University of Medicine and Pharmacy, 020956 Bucharest, RomaniaIn this study, we present a novel approach using amperometric microsensors to detect quercetin in cosmetic formulations and track its metabolic behavior after topical application. This method offers a sensitive, real-time alternative to conventional techniques, enabling the detection of quercetin’s bioavailability, its transformation into active metabolites, and its potential therapeutic effects when applied to the skin. Quercetin (Q) is a bioactive flavonoid known for its potent antioxidant properties, naturally present in numerous plants, particularly those with applications in cosmetic formulations. In response to the growing interest in developing novel plant-based dermo-cosmetic solutions, this study investigates the electrochemical detection of quercetin, a ketone-type flavonoid, extracted from Gingko biloba essential oil. Three newly designed amperometric microsensors were developed to assess their efficacy in detecting quercetin in botanical samples. The sensor configurations utilized two forms of carbon material as a foundation: graphite (G) and carbon nanoparticles (CNs). These base materials were modified with paraffin oil, chitosan (CHIT), and cobalt(II) tetraphenylporphyrin (Co(II)TPP) to enhance sensitivity. Differential pulse voltammetry (DPV) served as the analytical method for this investigation. Among the sensors, the CHIT/G–CN microsensor exhibited the highest sensitivity, with a detection limit of 1.22 × 10<sup>−7</sup> mol L<sup>−1</sup>, followed by the G–CN (5.64 × 10<sup>−8</sup> mol L<sup>−1</sup>) and Co(II)TPP/G–CN (9.80 × 10<sup>−8</sup> mol L<sup>−1</sup>) microsensors. The minimum detectable concentration was observed with the G–CN and CoP/G–CN microsensors, achieving a threshold as low as 0.0001 μmol L<sup>−1</sup>. Recovery rates and relative standard deviation (RSD) values averaged 97.4% ± 0.43, underscoring the sensors’ reliability for quercetin detection in botanical matrices.https://www.mdpi.com/2218-1989/15/1/6carbon nanopowderchitosandifferential pulse voltammetryessential oilsgraphitetetraphenyl-porphine cobalt (II) |
| spellingShingle | Elena Oancea Ioana Adina Tula Gabriela Stanciu Raluca-Ioana Ștefan-van Staden Jacobus (Koos) Frederick van Staden Magdalena Mititelu Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices Metabolites carbon nanopowder chitosan differential pulse voltammetry essential oils graphite tetraphenyl-porphine cobalt (II) |
| title | Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices |
| title_full | Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices |
| title_fullStr | Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices |
| title_full_unstemmed | Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices |
| title_short | Advanced Amperometric Microsensors for the Electrochemical Quantification of Quercetin in <i>Ginkgo biloba</i> Essential Oil from Regenerative Farming Practices |
| title_sort | advanced amperometric microsensors for the electrochemical quantification of quercetin in i ginkgo biloba i essential oil from regenerative farming practices |
| topic | carbon nanopowder chitosan differential pulse voltammetry essential oils graphite tetraphenyl-porphine cobalt (II) |
| url | https://www.mdpi.com/2218-1989/15/1/6 |
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