In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study
Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of R...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2017/2583969 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832552233775923200 |
|---|---|
| author | Emmanuel Mouafo Tekwu Kwabena Mante Bosompem William Kofi Anyan Regina Appiah-Opong Kofi Baffour-Awuah Owusu Mabel Deladem Tettey Felicia Amanfo Kissi Alfred Ampomah Appiah Veronique Penlap Beng Alexander Kwadwo Nyarko |
| author_facet | Emmanuel Mouafo Tekwu Kwabena Mante Bosompem William Kofi Anyan Regina Appiah-Opong Kofi Baffour-Awuah Owusu Mabel Deladem Tettey Felicia Amanfo Kissi Alfred Ampomah Appiah Veronique Penlap Beng Alexander Kwadwo Nyarko |
| author_sort | Emmanuel Mouafo Tekwu |
| collection | DOAJ |
| description | Schistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62.5–1000 µg/mL and 250–1000 µg/mL of R. vomitoria stem bark and roots, respectively. The LC50 values determined for the stem bark after 1 and 2 h of exposure were 207.4 and 61.18 µg/mL, respectively. All adult worms exposed to the concentrations range of 250–1000 µg/mL for both plant parts died within 120 h of incubation. The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC50 > 20 μg/mL appear to be safe. This report provides the first evidence of in vitro schistosomicidal potency of R. vomitoria with the stem bark being moderately, but relatively, more active and selective against schistosome parasites. This suggests the presence of promising medicinal constituent(s). |
| format | Article |
| id | doaj-art-85011da1c7f543388d44c2fb976fd718 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-85011da1c7f543388d44c2fb976fd7182025-02-03T05:59:16ZengWileyJournal of Parasitology Research2090-00232090-00312017-01-01201710.1155/2017/25839692583969In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic StudyEmmanuel Mouafo Tekwu0Kwabena Mante Bosompem1William Kofi Anyan2Regina Appiah-Opong3Kofi Baffour-Awuah Owusu4Mabel Deladem Tettey5Felicia Amanfo Kissi6Alfred Ampomah Appiah7Veronique Penlap Beng8Alexander Kwadwo Nyarko9Laboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, CameroonNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaNoguchi Memorial Institute for Medical Research (NMIMR), College of Health Sciences, University of Ghana, PO Box LG581 Legon, Accra, GhanaCentre for Plant Medicine Research (CPMR), Akwapim, Mampong, GhanaLaboratory for Tuberculosis Research and Pharmacology, Biotechnology Centre, Nkolbisson, University of Yaoundé 1, Yaoundé, CameroonSchool of Pharmacy, College of Health Sciences, University of Ghana, Accra, GhanaSchistosomiasis is a Neglected Tropical Diseases which can be prevented with mass deworming chemotherapy. The reliance on a single drug, praziquantel, is a motivation for the search of novel antischistosomal compounds. This study investigated the anthelmintic activity of the stem bark and roots of Rauwolfia vomitoria against two life stages of Schistosoma mansoni. Both plant parts were found to be active against cercariae and adult worms. Within 2 h of exposure all cercariae were killed at a concentration range of 62.5–1000 µg/mL and 250–1000 µg/mL of R. vomitoria stem bark and roots, respectively. The LC50 values determined for the stem bark after 1 and 2 h of exposure were 207.4 and 61.18 µg/mL, respectively. All adult worms exposed to the concentrations range of 250–1000 µg/mL for both plant parts died within 120 h of incubation. The cytotoxic effects against HepG2 and Chang liver cell assessed using MTT assay method indicated that both plant extracts which were inhibitory to the proliferation of cell lines with IC50 > 20 μg/mL appear to be safe. This report provides the first evidence of in vitro schistosomicidal potency of R. vomitoria with the stem bark being moderately, but relatively, more active and selective against schistosome parasites. This suggests the presence of promising medicinal constituent(s).http://dx.doi.org/10.1155/2017/2583969 |
| spellingShingle | Emmanuel Mouafo Tekwu Kwabena Mante Bosompem William Kofi Anyan Regina Appiah-Opong Kofi Baffour-Awuah Owusu Mabel Deladem Tettey Felicia Amanfo Kissi Alfred Ampomah Appiah Veronique Penlap Beng Alexander Kwadwo Nyarko In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study Journal of Parasitology Research |
| title | In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study |
| title_full | In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study |
| title_fullStr | In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study |
| title_full_unstemmed | In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study |
| title_short | In Vitro Assessment of Anthelmintic Activities of Rauwolfia vomitoria (Apocynaceae) Stem Bark and Roots against Parasitic Stages of Schistosoma mansoni and Cytotoxic Study |
| title_sort | in vitro assessment of anthelmintic activities of rauwolfia vomitoria apocynaceae stem bark and roots against parasitic stages of schistosoma mansoni and cytotoxic study |
| url | http://dx.doi.org/10.1155/2017/2583969 |
| work_keys_str_mv | AT emmanuelmouafotekwu invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT kwabenamantebosompem invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT williamkofianyan invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT reginaappiahopong invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT kofibaffourawuahowusu invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT mabeldelademtettey invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT feliciaamanfokissi invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT alfredampomahappiah invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT veroniquepenlapbeng invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy AT alexanderkwadwonyarko invitroassessmentofanthelminticactivitiesofrauwolfiavomitoriaapocynaceaestembarkandrootsagainstparasiticstagesofschistosomamansoniandcytotoxicstudy |