Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells

Selection and Engineering of Novel Brighter Bioluminescent Reporter Gene and Color- Tuning Luciferase for pH-Sensing in Mammalian Cells

Firefly luciferases have been extensively used for bioanalytical applications, including their use as bioluminescent reporters, biosensors, and for bioimaging biological and pathological processes. Due to their intrinsic pH- sensitivity, in recent years we have demonstrated that firefly luciferases...

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Bibliographic Details
Main Authors: Vanessa R. Bevilaqua, Gabriel F. Pelentir, Moema A. Hausen, Eliana A. R. Duek, Vadim R. Viviani
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Biosensors
Subjects:
bioluminescence
biosensors
intracellular events
Online Access:https://www.mdpi.com/2079-6374/15/1/18
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