Bioflocculant production from molasses by Photorhabdus luminescens, flocculation condition optimization and its application in heavy metal removal
A novel biopolymer flocculant was produced from molasses by Photorhabdus luminescens fermentation, and the medium was optimized to increase the yield of biomolecular flocculant by single factor test. Using flocculation rate as the response value, the effect of flocculant on the flocculation effect o...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Department of China Brewing
2025-01-01
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| Series: | Zhongguo niangzao |
| Subjects: | |
| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2025-44-1-247.pdf |
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| Summary: | A novel biopolymer flocculant was produced from molasses by Photorhabdus luminescens fermentation, and the medium was optimized to increase the yield of biomolecular flocculant by single factor test. Using flocculation rate as the response value, the effect of flocculant on the flocculation effect of kaolin suspension was studied by single factor test and response surface methodology. The results showed that the optimal medium composition was molasses 5 g/L, pepton 10 g/L, NaCl 5 g/L. Under these conditions, the flocculant yield reached 4.53 g/L. The optimal flocculation conditions were pH 7.0, biological flocculant 200 mg/L, cationic inducer (FeCl<sub>3</sub>) 50 mg/L and stirring rate 240 r/min. Under these conditions, the flocculation rate of flocculant on kaolin reached 93.2%. The removal rates of Cr, Pb, Ni, Cu and Cd were 61.32%, 58.22%, 85.90%, 78.18% and 80.71%, respectively. Molasses was used as carbon source for cheap medium of bioflocculant, which could reduce the culture cost and realize the reuse of waste residue. |
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| ISSN: | 0254-5071 |