Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza.
Vaccination is the most effective course of action to prevent influenza. About 150 million doses of influenza vaccines were distributed for the 2015-2016 season in the USA alone according to the Centers for Disease Control and Prevention. Vaccine dosage is calculated based on the concentration of he...
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Public Library of Science (PLoS)
2017-01-01
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| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180314&type=printable |
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| author | Aziza P Manceur Wei Zou Anne Marcil Eric Paquet Christine Gadoury Bozena Jaentschke Xuguang Li Emma Petiot Yves Durocher Jason Baardsnes Manuel Rosa-Calatrava Sven Ansorge Amine A Kamen |
| author_facet | Aziza P Manceur Wei Zou Anne Marcil Eric Paquet Christine Gadoury Bozena Jaentschke Xuguang Li Emma Petiot Yves Durocher Jason Baardsnes Manuel Rosa-Calatrava Sven Ansorge Amine A Kamen |
| author_sort | Aziza P Manceur |
| collection | DOAJ |
| description | Vaccination is the most effective course of action to prevent influenza. About 150 million doses of influenza vaccines were distributed for the 2015-2016 season in the USA alone according to the Centers for Disease Control and Prevention. Vaccine dosage is calculated based on the concentration of hemagglutinin (HA), the main surface glycoprotein expressed by influenza which varies from strain to strain. Therefore yearly-updated strain-specific antibodies and calibrating antigens are required. Preparing these quantification reagents can take up to three months and significantly slows down the release of new vaccine lots. Therefore, to circumvent the need for strain-specific sera, two anti-HA monoclonal antibodies (mAbs) against a highly conserved sequence have been produced by immunizing mice with a novel peptide-conjugate. Immunoblots demonstrate that 40 strains of influenza encompassing HA subtypes H1 to H13, as well as B strains from the Yamagata and Victoria lineage were detected when the two mAbs are combined to from a pan-HA mAb cocktail. Quantification using this pan-HA mAbs cocktail was achieved in a dot blot assay and results correlated with concentrations measured in a hemagglutination assay with a coefficient of correlation of 0.80. A competitive ELISA was also optimised with purified viral-like particles. Regardless of the quantification method used, pan-HA antibodies can be employed to accelerate process development when strain-specific antibodies are not available, and represent a valuable tool in case of pandemics. These antibodies were also expressed in CHO cells to facilitate large-scale production using bioreactor technologies which might be required to meet industrial needs for quantification reagents. Finally, a simulation model was created to predict the binding affinity of the two anti-HA antibodies to the amino acids composing the highly conserved epitope; different probabilities of interaction between a given amino acid and the antibodies might explain the affinity of each antibody against different influenza strains. |
| format | Article |
| id | doaj-art-84d646f3a5fe4208a105f5bca7c272fb |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-84d646f3a5fe4208a105f5bca7c272fb2025-08-20T02:45:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e018031410.1371/journal.pone.0180314Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza.Aziza P ManceurWei ZouAnne MarcilEric PaquetChristine GadouryBozena JaentschkeXuguang LiEmma PetiotYves DurocherJason BaardsnesManuel Rosa-CalatravaSven AnsorgeAmine A KamenVaccination is the most effective course of action to prevent influenza. About 150 million doses of influenza vaccines were distributed for the 2015-2016 season in the USA alone according to the Centers for Disease Control and Prevention. Vaccine dosage is calculated based on the concentration of hemagglutinin (HA), the main surface glycoprotein expressed by influenza which varies from strain to strain. Therefore yearly-updated strain-specific antibodies and calibrating antigens are required. Preparing these quantification reagents can take up to three months and significantly slows down the release of new vaccine lots. Therefore, to circumvent the need for strain-specific sera, two anti-HA monoclonal antibodies (mAbs) against a highly conserved sequence have been produced by immunizing mice with a novel peptide-conjugate. Immunoblots demonstrate that 40 strains of influenza encompassing HA subtypes H1 to H13, as well as B strains from the Yamagata and Victoria lineage were detected when the two mAbs are combined to from a pan-HA mAb cocktail. Quantification using this pan-HA mAbs cocktail was achieved in a dot blot assay and results correlated with concentrations measured in a hemagglutination assay with a coefficient of correlation of 0.80. A competitive ELISA was also optimised with purified viral-like particles. Regardless of the quantification method used, pan-HA antibodies can be employed to accelerate process development when strain-specific antibodies are not available, and represent a valuable tool in case of pandemics. These antibodies were also expressed in CHO cells to facilitate large-scale production using bioreactor technologies which might be required to meet industrial needs for quantification reagents. Finally, a simulation model was created to predict the binding affinity of the two anti-HA antibodies to the amino acids composing the highly conserved epitope; different probabilities of interaction between a given amino acid and the antibodies might explain the affinity of each antibody against different influenza strains.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180314&type=printable |
| spellingShingle | Aziza P Manceur Wei Zou Anne Marcil Eric Paquet Christine Gadoury Bozena Jaentschke Xuguang Li Emma Petiot Yves Durocher Jason Baardsnes Manuel Rosa-Calatrava Sven Ansorge Amine A Kamen Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. PLoS ONE |
| title | Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. |
| title_full | Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. |
| title_fullStr | Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. |
| title_full_unstemmed | Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. |
| title_short | Generation of monoclonal pan-hemagglutinin antibodies for the quantification of multiple strains of influenza. |
| title_sort | generation of monoclonal pan hemagglutinin antibodies for the quantification of multiple strains of influenza |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0180314&type=printable |
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