Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue
A neutral compound Re(CO)3(L) (L: 2-((2-(2,6-diethylphenylamino)-2-oxoethyl)(2-ethoxy-2-oxoethyl)amino)acetic acid, an IDA analogue) has been synthesized and evaluated for in vitro imaging probes of β-amyloid (Aβ) aggregates. Results of X-ray measurement of Re(CO)3(L) demonstrated that the coordina...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Bioinorganic Chemistry and Applications |
| Online Access: | http://dx.doi.org/10.1155/2009/702730 |
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| author | Yang Yang Jia Xin Zhang Lin Zhu Huabei Zhang |
| author_facet | Yang Yang Jia Xin Zhang Lin Zhu Huabei Zhang |
| author_sort | Yang Yang |
| collection | DOAJ |
| description | A neutral compound Re(CO)3(L)
(L: 2-((2-(2,6-diethylphenylamino)-2-oxoethyl)(2-ethoxy-2-oxoethyl)amino)acetic acid, an IDA analogue) has been synthesized and evaluated for in vitro imaging probes of β-amyloid (Aβ) aggregates. Results of X-ray measurement of Re(CO)3(L) demonstrated that the coordination mode of Re(CO)3(L) was different from that of classical Re/Tc(I) (tricarbonyl)-IDA analogues; the structure of Re(CO)3(L) was confirmed by means of infrared spectrum, HPLC-UV, TOF MS, and X-ray measurements (Cambridge Crystallographic Data Centre number is 732731): monoclinic P21/c, a=15.6636 (12) Å, b=10.9360 (8) Å, c=27.756 (2) Å, α=90.000 (0)∘, β=90.783 (5)∘, γ=90.000 (0)∘, and Z=8. The binding affinity for β-amyloid plaques was assessed by in vitro binding assay using preformed synthetic Aβ(1–40) aggregates. The neutral compound Re(CO)3(L) showed binding affinity to Aβ aggregates at micromolar level by fluorescence spectroscopy, and this work will encourage for further exploration of imaging agents labeled by T99mc(CO)3+ center as probes for β-amyloid plaques in vivo. |
| format | Article |
| id | doaj-art-84d0535a819f4972b024197204f2ad20 |
| institution | Kabale University |
| issn | 1565-3633 1687-479X |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Bioinorganic Chemistry and Applications |
| spelling | doaj-art-84d0535a819f4972b024197204f2ad202025-02-03T01:24:15ZengWileyBioinorganic Chemistry and Applications1565-36331687-479X2009-01-01200910.1155/2009/702730702730Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA AnalogueYang Yang0Jia Xin Zhang1Lin Zhu2Huabei Zhang3Key Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing 100875, ChinaKey Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing 100875, ChinaKey Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing 100875, ChinaKey Laboratory of Radiopharmaceuticals, Beijing Normal University, Ministry of Education, Beijing 100875, ChinaA neutral compound Re(CO)3(L) (L: 2-((2-(2,6-diethylphenylamino)-2-oxoethyl)(2-ethoxy-2-oxoethyl)amino)acetic acid, an IDA analogue) has been synthesized and evaluated for in vitro imaging probes of β-amyloid (Aβ) aggregates. Results of X-ray measurement of Re(CO)3(L) demonstrated that the coordination mode of Re(CO)3(L) was different from that of classical Re/Tc(I) (tricarbonyl)-IDA analogues; the structure of Re(CO)3(L) was confirmed by means of infrared spectrum, HPLC-UV, TOF MS, and X-ray measurements (Cambridge Crystallographic Data Centre number is 732731): monoclinic P21/c, a=15.6636 (12) Å, b=10.9360 (8) Å, c=27.756 (2) Å, α=90.000 (0)∘, β=90.783 (5)∘, γ=90.000 (0)∘, and Z=8. The binding affinity for β-amyloid plaques was assessed by in vitro binding assay using preformed synthetic Aβ(1–40) aggregates. The neutral compound Re(CO)3(L) showed binding affinity to Aβ aggregates at micromolar level by fluorescence spectroscopy, and this work will encourage for further exploration of imaging agents labeled by T99mc(CO)3+ center as probes for β-amyloid plaques in vivo.http://dx.doi.org/10.1155/2009/702730 |
| spellingShingle | Yang Yang Jia Xin Zhang Lin Zhu Huabei Zhang Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue Bioinorganic Chemistry and Applications |
| title | Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue |
| title_full | Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue |
| title_fullStr | Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue |
| title_full_unstemmed | Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue |
| title_short | Synthesis, Novel Crystal Structure, and β-Amyloid Binding Property of Re(I) (tricarbonyl)+ EHIDA Analogue |
| title_sort | synthesis novel crystal structure and β amyloid binding property of re i tricarbonyl ehida analogue |
| url | http://dx.doi.org/10.1155/2009/702730 |
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