Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice
The effect of high hydrostatic pressure (HHP) treatment combined with either of two natural inhibitors, ferulic acid (FA) or epigallocatechin gallate (EGCG) on polyphenol oxidase (PPO) activity and related quality attributes in cloudy apple juice was explored. The results showed that the addition of...
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China Food Publishing Company
2025-01-01
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| Series: | Shipin Kexue |
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| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-001.pdf |
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| author | TIAN Xuezhi, CAO Zhihong, JIANG Naixiang, XIAO Zhijian, TANG Zheng, WANG Yongtao |
| author_facet | TIAN Xuezhi, CAO Zhihong, JIANG Naixiang, XIAO Zhijian, TANG Zheng, WANG Yongtao |
| author_sort | TIAN Xuezhi, CAO Zhihong, JIANG Naixiang, XIAO Zhijian, TANG Zheng, WANG Yongtao |
| collection | DOAJ |
| description | The effect of high hydrostatic pressure (HHP) treatment combined with either of two natural inhibitors, ferulic acid (FA) or epigallocatechin gallate (EGCG) on polyphenol oxidase (PPO) activity and related quality attributes in cloudy apple juice was explored. The results showed that the addition of 0.1 g/100 mL FA or 0.2 g/100 mL EGCG effectively reduced PPO activity in samples, thereby significantly increasing brightness values (P < 0.05). After HHP treatment (550 MPa/5 min), the relative residual activity of PPO was as high as 82.70%, whereas it decreased to 66.35% and 41.50% after HHP + FA and HHP + EGCG treatment, respectively. During 14 days of refrigeration, PPO activity continued to decrease, while color parameters remained stable. On day 14, the HHP + FA group exhibited the lowest brown index (BI) of 4.37, while the HHP + EGCG group had the lowest relative residual PPO activity of 24.71%. Furthermore, both treatments retained more than 80% of the total phenol content and more than 90% of the antioxidant capacity. In conclusion, combined treatment of HHP with FA or EGCG could effectively inhibit the enzymatic browning of cloudy apple juice, demonstrating potential for further promotion and application. |
| format | Article |
| id | doaj-art-849b9c0c5cde40778398c22b65c25f27 |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-849b9c0c5cde40778398c22b65c25f272025-02-05T09:08:22ZengChina Food Publishing CompanyShipin Kexue1002-66302025-01-014621710.7506/spkx1002-6630-20240401-006Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple JuiceTIAN Xuezhi, CAO Zhihong, JIANG Naixiang, XIAO Zhijian, TANG Zheng, WANG Yongtao0(1. National Engineering Research Center for Fruit &Vegetable Processing, Key Laboratory of Fruit &Vegetable Processing, Ministry of Agriculture and Rural Affairs, Beijing Key Laboratory for Food Nonthermal Processing, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China; 2. Shandong Tailory Agricultural Technology Co. Ltd., Tai’an 271411, China)The effect of high hydrostatic pressure (HHP) treatment combined with either of two natural inhibitors, ferulic acid (FA) or epigallocatechin gallate (EGCG) on polyphenol oxidase (PPO) activity and related quality attributes in cloudy apple juice was explored. The results showed that the addition of 0.1 g/100 mL FA or 0.2 g/100 mL EGCG effectively reduced PPO activity in samples, thereby significantly increasing brightness values (P < 0.05). After HHP treatment (550 MPa/5 min), the relative residual activity of PPO was as high as 82.70%, whereas it decreased to 66.35% and 41.50% after HHP + FA and HHP + EGCG treatment, respectively. During 14 days of refrigeration, PPO activity continued to decrease, while color parameters remained stable. On day 14, the HHP + FA group exhibited the lowest brown index (BI) of 4.37, while the HHP + EGCG group had the lowest relative residual PPO activity of 24.71%. Furthermore, both treatments retained more than 80% of the total phenol content and more than 90% of the antioxidant capacity. In conclusion, combined treatment of HHP with FA or EGCG could effectively inhibit the enzymatic browning of cloudy apple juice, demonstrating potential for further promotion and application.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-001.pdfhigh hydrostatic pressure; natural inhibitors; cloudy apple juice; polyphenol oxidase; quality |
| spellingShingle | TIAN Xuezhi, CAO Zhihong, JIANG Naixiang, XIAO Zhijian, TANG Zheng, WANG Yongtao Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice Shipin Kexue high hydrostatic pressure; natural inhibitors; cloudy apple juice; polyphenol oxidase; quality |
| title | Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice |
| title_full | Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice |
| title_fullStr | Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice |
| title_full_unstemmed | Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice |
| title_short | Effect of High Hydrostatic Pressure Combined with Natural Inhibitors on Polyphenol Oxidase Activity and Related Quality Attributes of Cloudy Apple Juice |
| title_sort | effect of high hydrostatic pressure combined with natural inhibitors on polyphenol oxidase activity and related quality attributes of cloudy apple juice |
| topic | high hydrostatic pressure; natural inhibitors; cloudy apple juice; polyphenol oxidase; quality |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-001.pdf |
| work_keys_str_mv | AT tianxuezhicaozhihongjiangnaixiangxiaozhijiantangzhengwangyongtao effectofhighhydrostaticpressurecombinedwithnaturalinhibitorsonpolyphenoloxidaseactivityandrelatedqualityattributesofcloudyapplejuice |