Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China
The <i>Fusarium</i> species is an important plant pathogen that can cause plant diseases in grassland, leading to the degradation of grassland quality. However, the morphology of <i>Fusarium</i> is greatly affected by environmental factors, which makes it difficult to identif...
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2025-01-01
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| author | Yanzhu Gao Zhixiao Zhang Mei Ji Sangzi Ze Haodong Wang Bin Yang Lianrong Hu Ning Zhao |
| author_facet | Yanzhu Gao Zhixiao Zhang Mei Ji Sangzi Ze Haodong Wang Bin Yang Lianrong Hu Ning Zhao |
| author_sort | Yanzhu Gao |
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| description | The <i>Fusarium</i> species is an important plant pathogen that can cause plant diseases in grassland, leading to the degradation of grassland quality. However, the morphology of <i>Fusarium</i> is greatly affected by environmental factors, which makes it difficult to identify its species. In addition, the pathogenic ability of different <i>Fusarium</i> species in plants has not been fully studied. In this study, <i>Fusarium</i> isolates were obtained from grassland herbaceous plants via tissue separation. Through morphological means and based on ITS, <i>RPB2</i>, and <i>TEF-1</i> gene sequences, we compared and constructed polygenic phylogenetic trees to classify and identify the <i>Fusarium</i> species. In addition, the pathogenicity of different <i>Fusarium</i> species was also analyzed. The results showed that a total of 24 <i>Fusarium</i> strains were successfully isolated from grassland, from which ten species were identified: <i>F. flagelliforme</i>, <i>F. longifundum</i>, <i>F. clavum</i>, <i>F. scirpi</i>, <i>F. ipomoeae, F. oxysporum</i>, etc. and were included in four complexes: <i>Fusarium incarnatum-equiseti</i> species complex (FIESC), <i>Fusarium oxysporum</i> species complex (FOSC), <i>Fusarium tricinctum</i> species complex (FTSC), and <i>Fusarium sambucinum</i> species complex (FSAMSC). Pathogenicity tests demonstrated that except for <i>F. ipomoeae</i> QJ5211, <i>F. sambucinum</i> QJ203, and <i>F. acuminatum</i> QJ1662, other <i>Fusarium</i> species had different degrees of pathogenic ability. This is the first study that discusses the effect of <i>Fusarium</i> on grassland disease control in this area. This study further provides clear pathogen information for the prevention and control of grassland diseases. |
| format | Article |
| id | doaj-art-8496b12855a0452d994a8b044ce8e194 |
| institution | Kabale University |
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| language | English |
| publishDate | 2025-01-01 |
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| series | Microorganisms |
| spelling | doaj-art-8496b12855a0452d994a8b044ce8e1942025-01-24T13:42:42ZengMDPI AGMicroorganisms2076-26072025-01-0113111310.3390/microorganisms13010113Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, ChinaYanzhu Gao0Zhixiao Zhang1Mei Ji2Sangzi Ze3Haodong Wang4Bin Yang5Lianrong Hu6Ning Zhao7College of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaYunnan Academy of Forestry, Kunming 650224, ChinaYunnan Academy of Forestry, Kunming 650224, ChinaYunnan Forestry and Grassland Pest Control and Quarantine Bureau, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaSchool of Biological and Chemical Science, Pu’er University, Pu’er 665000, ChinaYunnan Academy of Forestry, Kunming 650224, ChinaCollege of Biological Science and Food Engineering, Southwest Forestry University, Kunming 650224, ChinaThe <i>Fusarium</i> species is an important plant pathogen that can cause plant diseases in grassland, leading to the degradation of grassland quality. However, the morphology of <i>Fusarium</i> is greatly affected by environmental factors, which makes it difficult to identify its species. In addition, the pathogenic ability of different <i>Fusarium</i> species in plants has not been fully studied. In this study, <i>Fusarium</i> isolates were obtained from grassland herbaceous plants via tissue separation. Through morphological means and based on ITS, <i>RPB2</i>, and <i>TEF-1</i> gene sequences, we compared and constructed polygenic phylogenetic trees to classify and identify the <i>Fusarium</i> species. In addition, the pathogenicity of different <i>Fusarium</i> species was also analyzed. The results showed that a total of 24 <i>Fusarium</i> strains were successfully isolated from grassland, from which ten species were identified: <i>F. flagelliforme</i>, <i>F. longifundum</i>, <i>F. clavum</i>, <i>F. scirpi</i>, <i>F. ipomoeae, F. oxysporum</i>, etc. and were included in four complexes: <i>Fusarium incarnatum-equiseti</i> species complex (FIESC), <i>Fusarium oxysporum</i> species complex (FOSC), <i>Fusarium tricinctum</i> species complex (FTSC), and <i>Fusarium sambucinum</i> species complex (FSAMSC). Pathogenicity tests demonstrated that except for <i>F. ipomoeae</i> QJ5211, <i>F. sambucinum</i> QJ203, and <i>F. acuminatum</i> QJ1662, other <i>Fusarium</i> species had different degrees of pathogenic ability. This is the first study that discusses the effect of <i>Fusarium</i> on grassland disease control in this area. This study further provides clear pathogen information for the prevention and control of grassland diseases.https://www.mdpi.com/2076-2607/13/1/113<i>Fusarium</i> complex species<i>F. oxysporum</i>gene locusplant pathogensmolecular phylogeny |
| spellingShingle | Yanzhu Gao Zhixiao Zhang Mei Ji Sangzi Ze Haodong Wang Bin Yang Lianrong Hu Ning Zhao Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China Microorganisms <i>Fusarium</i> complex species <i>F. oxysporum</i> gene locus plant pathogens molecular phylogeny |
| title | Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China |
| title_full | Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China |
| title_fullStr | Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China |
| title_full_unstemmed | Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China |
| title_short | Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China |
| title_sort | identification and pathogenicity of i fusarium i species from herbaceous plants on grassland in qiaojia county china |
| topic | <i>Fusarium</i> complex species <i>F. oxysporum</i> gene locus plant pathogens molecular phylogeny |
| url | https://www.mdpi.com/2076-2607/13/1/113 |
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