Identification and Pathogenicity of <i>Fusarium</i> Species from Herbaceous Plants on Grassland in Qiaojia County, China
The <i>Fusarium</i> species is an important plant pathogen that can cause plant diseases in grassland, leading to the degradation of grassland quality. However, the morphology of <i>Fusarium</i> is greatly affected by environmental factors, which makes it difficult to identif...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/1/113 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Summary: | The <i>Fusarium</i> species is an important plant pathogen that can cause plant diseases in grassland, leading to the degradation of grassland quality. However, the morphology of <i>Fusarium</i> is greatly affected by environmental factors, which makes it difficult to identify its species. In addition, the pathogenic ability of different <i>Fusarium</i> species in plants has not been fully studied. In this study, <i>Fusarium</i> isolates were obtained from grassland herbaceous plants via tissue separation. Through morphological means and based on ITS, <i>RPB2</i>, and <i>TEF-1</i> gene sequences, we compared and constructed polygenic phylogenetic trees to classify and identify the <i>Fusarium</i> species. In addition, the pathogenicity of different <i>Fusarium</i> species was also analyzed. The results showed that a total of 24 <i>Fusarium</i> strains were successfully isolated from grassland, from which ten species were identified: <i>F. flagelliforme</i>, <i>F. longifundum</i>, <i>F. clavum</i>, <i>F. scirpi</i>, <i>F. ipomoeae, F. oxysporum</i>, etc. and were included in four complexes: <i>Fusarium incarnatum-equiseti</i> species complex (FIESC), <i>Fusarium oxysporum</i> species complex (FOSC), <i>Fusarium tricinctum</i> species complex (FTSC), and <i>Fusarium sambucinum</i> species complex (FSAMSC). Pathogenicity tests demonstrated that except for <i>F. ipomoeae</i> QJ5211, <i>F. sambucinum</i> QJ203, and <i>F. acuminatum</i> QJ1662, other <i>Fusarium</i> species had different degrees of pathogenic ability. This is the first study that discusses the effect of <i>Fusarium</i> on grassland disease control in this area. This study further provides clear pathogen information for the prevention and control of grassland diseases. |
|---|---|
| ISSN: | 2076-2607 |