Impact of predictive selection of LbCas12a CRISPR RNAs upon on‐ and off‐target editing rates in soybean
Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence‐specificity, careful design of the editing reagents can also reduce unintended edit...
Saved in:
Main Authors: | Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega‐Sanchez, Thomas E. Mullen, Vijetha Vemulapalli, Douglas R. Smith |
---|---|
Format: | Article |
Language: | English |
Published: |
Wiley
2024-08-01
|
Series: | Plant Direct |
Subjects: | |
Online Access: | https://doi.org/10.1002/pld3.627 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
CRISPR-Cas9-based one-step multiplexed genome editing through optimizing guide RNA processing strategies in Pichia pastoris
by: Kaidi Chen, et al.
Published: (2025-06-01) -
Exploiting CRISPR/Cas system in controlling antibiotic resistance of bacteria
by: Pourya Gholizadeh, et al.
Published: (2024-07-01) -
FREKUENSI PEMBERIAN GROW QUICK LB TERHADAP PERTUMBUHAN BIBIT ANGGREK DENDROBIUM PADA STADIA KOMUNITAS POT
by: SURTINAH SURTINAH, et al.
Published: (2013-02-01) -
Synergistic strategies for glioblastoma treatment: CRISPR-based multigene editing combined with immune checkpoint blockade
by: Xiaolin Liu, et al.
Published: (2025-02-01) -
From bench to bedside: Developing CRISPR/Cas-based therapy for ocular diseases
by: Qing Zhao, et al.
Published: (2025-03-01)