Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen
The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. The particle size distribution, SEM, EDS, TEM, and FT-IR spectroscopy of...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Food Quality |
| Online Access: | http://dx.doi.org/10.1155/2019/7521895 |
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| _version_ | 1832566625458454528 |
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| author | Ling Zhang Xiaocui Yang Kaijun Xiao Yuyi Lu Chunhai Li Zijin Zhang |
| author_facet | Ling Zhang Xiaocui Yang Kaijun Xiao Yuyi Lu Chunhai Li Zijin Zhang |
| author_sort | Ling Zhang |
| collection | DOAJ |
| description | The structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. The particle size distribution, SEM, EDS, TEM, and FT-IR spectroscopy of the carrier before and after immobilization were analyzed. The results showed that the molecular weight of the purified enzyme protein was 31.0 kDa. The amino acid sequence with a consistency of 64.04% and one three-dimensional structure simulation diagram of the purified enzyme protein were obtained by LC-MS-MS, which suggested that the protein might belong to subtilisin. The optimal immobilization conditions were as follows: the volume ratio of the immobilization carrier to the enzyme was 3 : 50 (mL : mL), the immobilized temperature was 25°C, and the system pH was 4.5. Under this condition, the immobilization ratio of collagenase was 73.48%, the specific activity was 274.05 U/μg, and the specific activity of the immobilized enzyme was about 53.74% that of the free enzyme. The average particle size of SPS nanospheres was 155.1 nm. The characterization results of SEM, EDS, TEM, and FT-IR spectroscopy showed that the collagenase was successfully immobilized onto SPS nanospheres. The experimental results also showed that the collagenase could be immobilized effectively under the optimal conditions by using SPS nanospheres, and the operation process was simple, feasible, and of low cost with good prospect of industrial application. |
| format | Article |
| id | doaj-art-848dc9151e9f407ebd3213daff2390b9 |
| institution | Kabale University |
| issn | 0146-9428 1745-4557 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Food Quality |
| spelling | doaj-art-848dc9151e9f407ebd3213daff2390b92025-02-03T01:03:36ZengWileyJournal of Food Quality0146-94281745-45572019-01-01201910.1155/2019/75218957521895Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia CollagenLing Zhang0Xiaocui Yang1Kaijun Xiao2Yuyi Lu3Chunhai Li4Zijin Zhang5School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, ChinaSchool of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, ChinaSchool of Food Sciences and Engineering, South China University of Technology, Guangzhou 510640, Guangdong, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Food Science Innovation Team of Guangdong Higher Education Institutes, Maoming 525000, Guangdong, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Food Science Innovation Team of Guangdong Higher Education Institutes, Maoming 525000, Guangdong, ChinaCollege of Biological and Food Engineering, Guangdong University of Petrochemical Technology, Food Science Innovation Team of Guangdong Higher Education Institutes, Maoming 525000, Guangdong, ChinaThe structure of an alkaline protease from Bacillus subtilis used by a tilapia collagen peptide manufacturer was analyzed, and the technology of the enzyme immobilized by sulfonated polystyrene (SPS) nanoparticles was studied. The particle size distribution, SEM, EDS, TEM, and FT-IR spectroscopy of the carrier before and after immobilization were analyzed. The results showed that the molecular weight of the purified enzyme protein was 31.0 kDa. The amino acid sequence with a consistency of 64.04% and one three-dimensional structure simulation diagram of the purified enzyme protein were obtained by LC-MS-MS, which suggested that the protein might belong to subtilisin. The optimal immobilization conditions were as follows: the volume ratio of the immobilization carrier to the enzyme was 3 : 50 (mL : mL), the immobilized temperature was 25°C, and the system pH was 4.5. Under this condition, the immobilization ratio of collagenase was 73.48%, the specific activity was 274.05 U/μg, and the specific activity of the immobilized enzyme was about 53.74% that of the free enzyme. The average particle size of SPS nanospheres was 155.1 nm. The characterization results of SEM, EDS, TEM, and FT-IR spectroscopy showed that the collagenase was successfully immobilized onto SPS nanospheres. The experimental results also showed that the collagenase could be immobilized effectively under the optimal conditions by using SPS nanospheres, and the operation process was simple, feasible, and of low cost with good prospect of industrial application.http://dx.doi.org/10.1155/2019/7521895 |
| spellingShingle | Ling Zhang Xiaocui Yang Kaijun Xiao Yuyi Lu Chunhai Li Zijin Zhang Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen Journal of Food Quality |
| title | Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen |
| title_full | Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen |
| title_fullStr | Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen |
| title_full_unstemmed | Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen |
| title_short | Immobilization of Alkaline Collagenase from Bacillus subtilis onto Sulfonated Polystyrene Nanospheres for Hydrolysis of Tilapia Collagen |
| title_sort | immobilization of alkaline collagenase from bacillus subtilis onto sulfonated polystyrene nanospheres for hydrolysis of tilapia collagen |
| url | http://dx.doi.org/10.1155/2019/7521895 |
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