Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions
Since lead can cause severe effects on living organisms’ health and life, the regular monitoring of Pb levels in water and soil is of particular significance. Recently, it was shown that lead ions can also be detected using affinity-based biosensors, namely, using aptamers as recognition elements. I...
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| Language: | English |
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MDPI AG
2025-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/15/1/59 |
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| author | Izabela Zaras Olga Kujawa Marcin Olszewski Marta Jarczewska |
| author_facet | Izabela Zaras Olga Kujawa Marcin Olszewski Marta Jarczewska |
| author_sort | Izabela Zaras |
| collection | DOAJ |
| description | Since lead can cause severe effects on living organisms’ health and life, the regular monitoring of Pb levels in water and soil is of particular significance. Recently, it was shown that lead ions can also be detected using affinity-based biosensors, namely, using aptamers as recognition elements. In most cases, thrombin binding aptamer (TBA) was utilized; however, there are more examples of DNA aptamers which could also serve that purpose. Herein, we present studies on the electrochemical detection of lead ions using PS2M aptamer, which contains several guanine nucleotides, as the receptor element. Firstly, the method of aptamer-based layer fabrication was optimized along with the choice of a redox active indicator, which was a source of current signal. The experiments revealed the possibility of lead ion detection from 50 to 600 nM, which covers the range below and above the maximum accepted limit stated by US EPA (72 nM). Moreover, the sensing layer exhibited high selectivity towards lead ions and was successfully applied both for the analysis of tap water spiked with Pb<sup>2+</sup> ions and as a miniaturized sensor. Finally, stability and regeneration studies on the aptamer-based receptor layer were executed to confirm the utility of the elaborated tool. |
| format | Article |
| id | doaj-art-8453b1c73f804d298d7e90275f1004db |
| institution | Kabale University |
| issn | 2079-6374 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj-art-8453b1c73f804d298d7e90275f1004db2025-01-24T13:25:35ZengMDPI AGBiosensors2079-63742025-01-011515910.3390/bios15010059Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead IonsIzabela Zaras0Olga Kujawa1Marcin Olszewski2Marta Jarczewska3Chair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, PolandChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, PolandChair of Drug and Cosmetics Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Koszykowa 75, 00-664 Warsaw, PolandChair of Medical Biotechnology, Faculty of Chemistry, Warsaw University of Technology, Noakowskiego 3, 00-664 Warsaw, PolandSince lead can cause severe effects on living organisms’ health and life, the regular monitoring of Pb levels in water and soil is of particular significance. Recently, it was shown that lead ions can also be detected using affinity-based biosensors, namely, using aptamers as recognition elements. In most cases, thrombin binding aptamer (TBA) was utilized; however, there are more examples of DNA aptamers which could also serve that purpose. Herein, we present studies on the electrochemical detection of lead ions using PS2M aptamer, which contains several guanine nucleotides, as the receptor element. Firstly, the method of aptamer-based layer fabrication was optimized along with the choice of a redox active indicator, which was a source of current signal. The experiments revealed the possibility of lead ion detection from 50 to 600 nM, which covers the range below and above the maximum accepted limit stated by US EPA (72 nM). Moreover, the sensing layer exhibited high selectivity towards lead ions and was successfully applied both for the analysis of tap water spiked with Pb<sup>2+</sup> ions and as a miniaturized sensor. Finally, stability and regeneration studies on the aptamer-based receptor layer were executed to confirm the utility of the elaborated tool.https://www.mdpi.com/2079-6374/15/1/59aptamer-sensing layervoltammetryredox indicatorsmodified electrodes |
| spellingShingle | Izabela Zaras Olga Kujawa Marcin Olszewski Marta Jarczewska Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions Biosensors aptamer-sensing layer voltammetry redox indicators modified electrodes |
| title | Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions |
| title_full | Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions |
| title_fullStr | Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions |
| title_full_unstemmed | Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions |
| title_short | Application of PS2M Aptamer as Receptor Layer for Electrochemical Detection of Lead Ions |
| title_sort | application of ps2m aptamer as receptor layer for electrochemical detection of lead ions |
| topic | aptamer-sensing layer voltammetry redox indicators modified electrodes |
| url | https://www.mdpi.com/2079-6374/15/1/59 |
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