The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway
Previous studies have shown that the endogenous electric field (EF) is an overriding cure in guiding cell migration toward the wound center to promote wound healing, but the mechanism underlying is unclear. In this study, we investigated the molecular mechanism of electric field-guided cell migratio...
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MDPI AG
2024-12-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/47/1/16 |
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| author | Huajian Zhou Shihao Zhang Xiaoli Jin Chunxian A Peng Gong Sanjun Zhao |
| author_facet | Huajian Zhou Shihao Zhang Xiaoli Jin Chunxian A Peng Gong Sanjun Zhao |
| author_sort | Huajian Zhou |
| collection | DOAJ |
| description | Previous studies have shown that the endogenous electric field (EF) is an overriding cure in guiding cell migration toward the wound center to promote wound healing, but the mechanism underlying is unclear. In this study, we investigated the molecular mechanism of electric field-guided cell migration in human keratinocyte HaCaT cells. Our results showed that HaCaT cells migrate toward the anode under EFs. The phosphorylation levels of p38 MAPK and Akt were obviously elevated in the EF. Knocking down p38 MAPK obviously abolished directed migration of HaCaT cells under the EFs. Inhibiting p38 MAPK by SB203580 impaired the EF-guided cell migration. The electric field may guide HaCaT cell migration through the EGFR/p38 MAPK/Akt pathway. |
| format | Article |
| id | doaj-art-8436b1b6c58c48728fd79f7ff88ed540 |
| institution | Kabale University |
| issn | 1467-3037 1467-3045 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Current Issues in Molecular Biology |
| spelling | doaj-art-8436b1b6c58c48728fd79f7ff88ed5402025-01-24T13:27:24ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-12-014711610.3390/cimb47010016The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt PathwayHuajian Zhou0Shihao Zhang1Xiaoli Jin2Chunxian A3Peng Gong4Sanjun Zhao5School of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaSchool of Life Sciences, Yunnan Normal University, Kunming 650500, ChinaPrevious studies have shown that the endogenous electric field (EF) is an overriding cure in guiding cell migration toward the wound center to promote wound healing, but the mechanism underlying is unclear. In this study, we investigated the molecular mechanism of electric field-guided cell migration in human keratinocyte HaCaT cells. Our results showed that HaCaT cells migrate toward the anode under EFs. The phosphorylation levels of p38 MAPK and Akt were obviously elevated in the EF. Knocking down p38 MAPK obviously abolished directed migration of HaCaT cells under the EFs. Inhibiting p38 MAPK by SB203580 impaired the EF-guided cell migration. The electric field may guide HaCaT cell migration through the EGFR/p38 MAPK/Akt pathway.https://www.mdpi.com/1467-3045/47/1/16electric fieldcell migrationEGFRp38 MAPKAkt |
| spellingShingle | Huajian Zhou Shihao Zhang Xiaoli Jin Chunxian A Peng Gong Sanjun Zhao The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway Current Issues in Molecular Biology electric field cell migration EGFR p38 MAPK Akt |
| title | The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway |
| title_full | The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway |
| title_fullStr | The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway |
| title_full_unstemmed | The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway |
| title_short | The Electric Field Guided HaCaT Cell Migration Through the EGFR/p38 MAPK/Akt Pathway |
| title_sort | electric field guided hacat cell migration through the egfr p38 mapk akt pathway |
| topic | electric field cell migration EGFR p38 MAPK Akt |
| url | https://www.mdpi.com/1467-3045/47/1/16 |
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