Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB
INTRODUCTION: Non-tuberculous mycobacteria (NTM) are increasingly isolated in individuals with presumed/confirmed pulmonary TB. We aimed to estimate the prevalence and species distribution of NTM among presumed/confirmed drug-resistant TB (DR-TB) individuals and determine NTM isolation predictors. M...
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International Union Against Tuberculosis and Lung Disease (The Union)
2024-07-01
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| Series: | IJTLD Open |
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| Online Access: | https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2024/00000001/00000007/art00005 |
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| author | E.T. Abbew R. Laryea F. Sorvor Y.A. Poku N. Lorent D. Obiri-Yeboah L. Lynen L. Rigouts |
| author_facet | E.T. Abbew R. Laryea F. Sorvor Y.A. Poku N. Lorent D. Obiri-Yeboah L. Lynen L. Rigouts |
| author_sort | E.T. Abbew |
| collection | DOAJ |
| description | INTRODUCTION: Non-tuberculous mycobacteria (NTM) are increasingly isolated in individuals with presumed/confirmed pulmonary TB. We aimed to estimate the prevalence and species distribution of NTM among presumed/confirmed drug-resistant TB (DR-TB) individuals and determine NTM isolation predictors. METHODS: Sputum samples collected for DR-TB diagnosis and follow-up from 2012 to 2021 in Ghana were retrospectively analysed. Samples were subjected to sputum smear microscopy (SSM) and mycobacterial culture. The MPT64 assay was performed on positive cultures to distinguish between Mycobacterium tuberculosis complex MTBc and NTM. NTM isolates were re-cultured for species identification using GenoType® Mycobacterium CM/AS line-probe assay, polymerase chain reaction, and Sanger sequencing targeting 16S rRNA and rpoB genes. MTBc isolates identified by GenoType underwent spoligotyping. A logistic regression model was used to identify the predictors of NTM isolation. RESULTS: Of the 2,492 samples, 839 (33.7%) tested culture-positive for mycobacteria, with 257 (30.6%) presumed to be NTM. Of these, 53 (23.6%) were identified at the species level, with a predominance of M. intracellulare (66.0%). MPT64 testing missed 18 (3%) MTBc isolates. Logistic regression showed increased odds of NTM isolation in follow-up samples (aOR 2.41, 95% CI 1.46–3.99). NTM species were isolated from 46 patients, with four classified as NTM pulmonary disease CONCLUSION: Enhancing our understanding of local NTM epidemiology and improving local diagnostic capabilities can optimise patient management strategies and outcomes. |
| format | Article |
| id | doaj-art-841ed0f5fee948e8970d0ce00ff7aa61 |
| institution | Kabale University |
| issn | 3005-7590 |
| language | English |
| publishDate | 2024-07-01 |
| publisher | International Union Against Tuberculosis and Lung Disease (The Union) |
| record_format | Article |
| series | IJTLD Open |
| spelling | doaj-art-841ed0f5fee948e8970d0ce00ff7aa612025-01-22T12:12:38ZengInternational Union Against Tuberculosis and Lung Disease (The Union)IJTLD Open3005-75902024-07-011730631310.5588/ijtldopen.24.02425Prevalence and predictors of NTM in presumed/confirmed drug-resistant TBE.T. Abbew0R. Laryea1F. Sorvor2Y.A. Poku3N. Lorent4D. Obiri-Yeboah5L. Lynen6L. Rigouts7Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium;Eastern Regional Hospital, Koforidua, Ghana;National Tuberculosis Control Programme, Accra, Ghana;National Tuberculosis Control Programme, Accra, Ghana;Department of Respiratory Diseases, University Hospital Leuven, Leuven, Belgium;Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Ghana;Department of Clinical Sciences, Institute of Tropical Medicine, Antwerp, Belgium;Department of Biomedical Sciences, University of Antwerp, Belgium;INTRODUCTION: Non-tuberculous mycobacteria (NTM) are increasingly isolated in individuals with presumed/confirmed pulmonary TB. We aimed to estimate the prevalence and species distribution of NTM among presumed/confirmed drug-resistant TB (DR-TB) individuals and determine NTM isolation predictors. METHODS: Sputum samples collected for DR-TB diagnosis and follow-up from 2012 to 2021 in Ghana were retrospectively analysed. Samples were subjected to sputum smear microscopy (SSM) and mycobacterial culture. The MPT64 assay was performed on positive cultures to distinguish between Mycobacterium tuberculosis complex MTBc and NTM. NTM isolates were re-cultured for species identification using GenoType® Mycobacterium CM/AS line-probe assay, polymerase chain reaction, and Sanger sequencing targeting 16S rRNA and rpoB genes. MTBc isolates identified by GenoType underwent spoligotyping. A logistic regression model was used to identify the predictors of NTM isolation. RESULTS: Of the 2,492 samples, 839 (33.7%) tested culture-positive for mycobacteria, with 257 (30.6%) presumed to be NTM. Of these, 53 (23.6%) were identified at the species level, with a predominance of M. intracellulare (66.0%). MPT64 testing missed 18 (3%) MTBc isolates. Logistic regression showed increased odds of NTM isolation in follow-up samples (aOR 2.41, 95% CI 1.46–3.99). NTM species were isolated from 46 patients, with four classified as NTM pulmonary disease CONCLUSION: Enhancing our understanding of local NTM epidemiology and improving local diagnostic capabilities can optimise patient management strategies and outcomes.https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2024/00000001/00000007/art00005non-tuberculous mycobacterianon-tuberculous mycobacterial pulmonary diseasedrug-resistant tuberculosis |
| spellingShingle | E.T. Abbew R. Laryea F. Sorvor Y.A. Poku N. Lorent D. Obiri-Yeboah L. Lynen L. Rigouts Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB IJTLD Open non-tuberculous mycobacteria non-tuberculous mycobacterial pulmonary disease drug-resistant tuberculosis |
| title | Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB |
| title_full | Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB |
| title_fullStr | Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB |
| title_full_unstemmed | Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB |
| title_short | Prevalence and predictors of NTM in presumed/confirmed drug-resistant TB |
| title_sort | prevalence and predictors of ntm in presumed confirmed drug resistant tb |
| topic | non-tuberculous mycobacteria non-tuberculous mycobacterial pulmonary disease drug-resistant tuberculosis |
| url | https://www.ingentaconnect.com/contentone/iuatld/ijtldo/2024/00000001/00000007/art00005 |
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