Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T
Strain E19T described as Hartmannibacter diazotrophicus gen. nov. sp. nov. was isolated from the rhizosphere of Plantago winteri from a natural salt meadow in a nature protection area. Strain E19T is a plant growth-promoting rhizobacterium able to colonize the rhizosphere of barley and to promote it...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2019/7586430 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832548471236722688 |
|---|---|
| author | Christian Suarez Stefan Ratering Torsten Hain Moritz Fritzenwanker Alexander Goesmann Jochen Blom Trinad Chakraborty Boyke Bunk Cathrin Spröer Jörg Overmann Sylvia Schnell |
| author_facet | Christian Suarez Stefan Ratering Torsten Hain Moritz Fritzenwanker Alexander Goesmann Jochen Blom Trinad Chakraborty Boyke Bunk Cathrin Spröer Jörg Overmann Sylvia Schnell |
| author_sort | Christian Suarez |
| collection | DOAJ |
| description | Strain E19T described as Hartmannibacter diazotrophicus gen. nov. sp. nov. was isolated from the rhizosphere of Plantago winteri from a natural salt meadow in a nature protection area. Strain E19T is a plant growth-promoting rhizobacterium able to colonize the rhizosphere of barley and to promote its growth only under salt stress conditions. To gain insights into the genetic bases of plant growth promotion and its lifestyle at the rhizosphere under salty conditions, we determined the complete genome sequence using two complementary sequencing platforms (Ilumina MiSeq and PacBio RSII). The E19T genome comprises one circular chromosome and one plasmid containing several genes involved in salt adaptation and genes related to plant growth-promoting traits under salt stress. Based on previous experiments, ACC deaminase activity was identified as a main mechanism of E19T to promote plant growth under salt stress. Interestingly, no genes classically reported to encode for ACC deaminase activity are present. In general, the E19T genome provides information to confirm, discover, and better understand many of its previously evaluated traits involved in plant growth promotion under salt stress. Furthermore, the complete E19T genome sequence helps to define its previously reported unclear 16S rRNA gene-based phylogenetic affiliation. Hartmannibacter forms a distinct subcluster with genera Methylobrevis, Pleomorphomonas, Oharaeibacter, and Mongoliimonas subclustered with genera belonging to Rhizobiales. |
| format | Article |
| id | doaj-art-83f6c0765691453a89f6e2aefefc79e3 |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-83f6c0765691453a89f6e2aefefc79e32025-02-03T06:13:57ZengWileyInternational Journal of Genomics2314-436X2314-43782019-01-01201910.1155/2019/75864307586430Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19TChristian Suarez0Stefan Ratering1Torsten Hain2Moritz Fritzenwanker3Alexander Goesmann4Jochen Blom5Trinad Chakraborty6Boyke Bunk7Cathrin Spröer8Jörg Overmann9Sylvia Schnell10Institute of Applied Microbiology, IFZ, Justus Liebig University Giessen, 35392 Giessen, GermanyInstitute of Applied Microbiology, IFZ, Justus Liebig University Giessen, 35392 Giessen, GermanyInstitute of Medical Microbiology, BFS, Justus Liebig University Giessen, 35392 Giessen, GermanyInstitute of Medical Microbiology, BFS, Justus Liebig University Giessen, 35392 Giessen, GermanyBioinformatics and Systems Biology, Justus Liebig University Giessen, 35392 Giessen, GermanyBioinformatics and Systems Biology, Justus Liebig University Giessen, 35392 Giessen, GermanyInstitute of Medical Microbiology, BFS, Justus Liebig University Giessen, 35392 Giessen, GermanyLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyLeibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, GermanyInstitute of Applied Microbiology, IFZ, Justus Liebig University Giessen, 35392 Giessen, GermanyStrain E19T described as Hartmannibacter diazotrophicus gen. nov. sp. nov. was isolated from the rhizosphere of Plantago winteri from a natural salt meadow in a nature protection area. Strain E19T is a plant growth-promoting rhizobacterium able to colonize the rhizosphere of barley and to promote its growth only under salt stress conditions. To gain insights into the genetic bases of plant growth promotion and its lifestyle at the rhizosphere under salty conditions, we determined the complete genome sequence using two complementary sequencing platforms (Ilumina MiSeq and PacBio RSII). The E19T genome comprises one circular chromosome and one plasmid containing several genes involved in salt adaptation and genes related to plant growth-promoting traits under salt stress. Based on previous experiments, ACC deaminase activity was identified as a main mechanism of E19T to promote plant growth under salt stress. Interestingly, no genes classically reported to encode for ACC deaminase activity are present. In general, the E19T genome provides information to confirm, discover, and better understand many of its previously evaluated traits involved in plant growth promotion under salt stress. Furthermore, the complete E19T genome sequence helps to define its previously reported unclear 16S rRNA gene-based phylogenetic affiliation. Hartmannibacter forms a distinct subcluster with genera Methylobrevis, Pleomorphomonas, Oharaeibacter, and Mongoliimonas subclustered with genera belonging to Rhizobiales.http://dx.doi.org/10.1155/2019/7586430 |
| spellingShingle | Christian Suarez Stefan Ratering Torsten Hain Moritz Fritzenwanker Alexander Goesmann Jochen Blom Trinad Chakraborty Boyke Bunk Cathrin Spröer Jörg Overmann Sylvia Schnell Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T International Journal of Genomics |
| title | Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T |
| title_full | Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T |
| title_fullStr | Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T |
| title_full_unstemmed | Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T |
| title_short | Complete Genome Sequence of the Plant Growth-Promoting Bacterium Hartmannibacter diazotrophicus Strain E19T |
| title_sort | complete genome sequence of the plant growth promoting bacterium hartmannibacter diazotrophicus strain e19t |
| url | http://dx.doi.org/10.1155/2019/7586430 |
| work_keys_str_mv | AT christiansuarez completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT stefanratering completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT torstenhain completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT moritzfritzenwanker completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT alexandergoesmann completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT jochenblom completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT trinadchakraborty completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT boykebunk completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT cathrinsproer completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT jorgovermann completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t AT sylviaschnell completegenomesequenceoftheplantgrowthpromotingbacteriumhartmannibacterdiazotrophicusstraine19t |