Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs
After insemination, a subpopulation of sperm reaches the oviducts and binds to isthmic epithelial cells to form a “sperm reservoir”. Our objective was to explore the role of annexin A5 (ANXA5), a protein that binds with high affinity to phosphatidylserine (PS), in the formation of the sperm reservoi...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-03-01
|
| Series: | European Journal of Cell Biology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0171933524000888 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832590967068164096 |
|---|---|
| author | Lorraine Schmaltz Elie Barakat Renaud Fleurot Rustem Uzbekov Karine Reynaud Ludivine Laffont Guillaume Tsikis Isabelle Mérour Pascal Mermillod Marie Saint-Dizier |
| author_facet | Lorraine Schmaltz Elie Barakat Renaud Fleurot Rustem Uzbekov Karine Reynaud Ludivine Laffont Guillaume Tsikis Isabelle Mérour Pascal Mermillod Marie Saint-Dizier |
| author_sort | Lorraine Schmaltz |
| collection | DOAJ |
| description | After insemination, a subpopulation of sperm reaches the oviducts and binds to isthmic epithelial cells to form a “sperm reservoir”. Our objective was to explore the role of annexin A5 (ANXA5), a protein that binds with high affinity to phosphatidylserine (PS), in the formation of the sperm reservoir in pigs.Phosphatidylserine was detected on the head of approximately 10 % of boar sperm at ejaculation. Porcine ANXA5 was immunodetected with a strong signal on luminal cilia in the isthmus and in derived isthmic epithelial spheroids (IES). Exogenous PS between 0.01 and 0.1 µg/mL and recombinant porcine ANXA5 (rpANXA5) above 0.1 µg/mL inhibited sperm binding to IES without reducing sperm motility. Pre-incubation of sperm, but not IES, with rpANXA5 inhibited sperm binding to IES. Under capacitating conditions, the proportion of live sperm with head PS exposure and the ability of sperm to bind to rpANXA5 and IES cilia increased within 30 min. Conversely, the acrosome reaction decreased the ability of sperm to bind rpANXA5 and prevented sperm binding to IES.In conclusion, sperm membrane remodelling during capacitation enhanced head PS exposure in motile sperm, resulting in increased interaction with ciliary ANXA5 on isthmic epithelial spheroids. These findings support a role for PS-ANXA5 interaction in the formation of the sperm reservoir in mammalian females. |
| format | Article |
| id | doaj-art-83cc889e9376471191ef09828166b41f |
| institution | Kabale University |
| issn | 0171-9335 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | European Journal of Cell Biology |
| spelling | doaj-art-83cc889e9376471191ef09828166b41f2025-01-23T05:26:15ZengElsevierEuropean Journal of Cell Biology0171-93352025-03-011041151471Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigsLorraine Schmaltz0Elie Barakat1Renaud Fleurot2Rustem Uzbekov3Karine Reynaud4Ludivine Laffont5Guillaume Tsikis6Isabelle Mérour7Pascal Mermillod8Marie Saint-Dizier9INRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceLaboratory of Cell Biology and Electron Microscopy, University of Tours, Tours, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceYXIA, St-Gilles, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, FranceINRAE, CNRS, University of Tours, Physiologie de la Reproduction et des comportements, Center INRAE Val-de-Loire, Nouzilly, France; Corresponding author.After insemination, a subpopulation of sperm reaches the oviducts and binds to isthmic epithelial cells to form a “sperm reservoir”. Our objective was to explore the role of annexin A5 (ANXA5), a protein that binds with high affinity to phosphatidylserine (PS), in the formation of the sperm reservoir in pigs.Phosphatidylserine was detected on the head of approximately 10 % of boar sperm at ejaculation. Porcine ANXA5 was immunodetected with a strong signal on luminal cilia in the isthmus and in derived isthmic epithelial spheroids (IES). Exogenous PS between 0.01 and 0.1 µg/mL and recombinant porcine ANXA5 (rpANXA5) above 0.1 µg/mL inhibited sperm binding to IES without reducing sperm motility. Pre-incubation of sperm, but not IES, with rpANXA5 inhibited sperm binding to IES. Under capacitating conditions, the proportion of live sperm with head PS exposure and the ability of sperm to bind to rpANXA5 and IES cilia increased within 30 min. Conversely, the acrosome reaction decreased the ability of sperm to bind rpANXA5 and prevented sperm binding to IES.In conclusion, sperm membrane remodelling during capacitation enhanced head PS exposure in motile sperm, resulting in increased interaction with ciliary ANXA5 on isthmic epithelial spheroids. These findings support a role for PS-ANXA5 interaction in the formation of the sperm reservoir in mammalian females.http://www.sciencedirect.com/science/article/pii/S0171933524000888SpermatozoaOviductPhosphatidylserineAnnexin A5CapacitationSperm storage |
| spellingShingle | Lorraine Schmaltz Elie Barakat Renaud Fleurot Rustem Uzbekov Karine Reynaud Ludivine Laffont Guillaume Tsikis Isabelle Mérour Pascal Mermillod Marie Saint-Dizier Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs European Journal of Cell Biology Spermatozoa Oviduct Phosphatidylserine Annexin A5 Capacitation Sperm storage |
| title | Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| title_full | Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| title_fullStr | Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| title_full_unstemmed | Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| title_short | Phosphatidylserine on sperm head interact with Annexin A5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| title_sort | phosphatidylserine on sperm head interact with annexin a5 on oviduct luminal cilia to form a sperm reservoir in pigs |
| topic | Spermatozoa Oviduct Phosphatidylserine Annexin A5 Capacitation Sperm storage |
| url | http://www.sciencedirect.com/science/article/pii/S0171933524000888 |
| work_keys_str_mv | AT lorraineschmaltz phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT eliebarakat phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT renaudfleurot phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT rustemuzbekov phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT karinereynaud phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT ludivinelaffont phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT guillaumetsikis phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT isabellemerour phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT pascalmermillod phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs AT mariesaintdizier phosphatidylserineonspermheadinteractwithannexina5onoviductluminalciliatoformaspermreservoirinpigs |