Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367
Inflammation has always been considered a trigger or consequence of neurodegenerative diseases, and the inhibition of inflammation in the central nervous system can effectively protect nerve cells. Several studies have indicated that various natural products inhibit neuroinflammation. Among these, A...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-01-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/30/2/294 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832587861999747072 |
|---|---|
| author | Zhiming Liu Chi-Su Yoon Thao Quyen Cao Hwan Lee Il-Chan Kim Joung Han Yim Jae Hak Sohn Dong-Sung Lee Hyuncheol Oh |
| author_facet | Zhiming Liu Chi-Su Yoon Thao Quyen Cao Hwan Lee Il-Chan Kim Joung Han Yim Jae Hak Sohn Dong-Sung Lee Hyuncheol Oh |
| author_sort | Zhiming Liu |
| collection | DOAJ |
| description | Inflammation has always been considered a trigger or consequence of neurodegenerative diseases, and the inhibition of inflammation in the central nervous system can effectively protect nerve cells. Several studies have indicated that various natural products inhibit neuroinflammation. Among these, Antarctic fungal metabolites have pharmacological activities and a developmental value. Therefore, this study aimed to evaluate the anti-neuroinflammatory activity of an Antarctic fungus belonging to <i>Aspergillus</i> (strain SF-7367). Secondary metabolites of SF-7367 were isolated using high-performance liquid chromatography followed by validation of their anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglia and RAW264.7 macrophages. Chemical analysis of metabolites from the fungal strain revealed five known compounds: epideoxybrevianamide E (<b>1</b>), brevianamide V/W (<b>2</b>), brevianamide K (<b>3</b>), brevianamide Q (<b>4</b>), and brevianamide R (<b>5</b>). Among these compounds, brevianamide K showed significant anti-inflammatory activity against both cell types. Results of Western blotting and molecular docking showed that brevianamide K could regulate the activation of nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) signaling. This indicates that brevianamide K present in <i>Aspergillus</i> sp. (strain SF-7367) can inhibit inflammatory responses by reducing lipopolysaccharide-induced nuclear translocation of NF-κB (p65). These findings suggest that <i>Aspergillus</i> sp. (strain SF-7367) and brevianamide K are candidate agents for treating neurodegenerative diseases. |
| format | Article |
| id | doaj-art-8375f5104be646b0abbe841ce2fb2163 |
| institution | Kabale University |
| issn | 1420-3049 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj-art-8375f5104be646b0abbe841ce2fb21632025-01-24T13:43:27ZengMDPI AGMolecules1420-30492025-01-0130229410.3390/molecules30020294Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367Zhiming Liu0Chi-Su Yoon1Thao Quyen Cao2Hwan Lee3Il-Chan Kim4Joung Han Yim5Jae Hak Sohn6Dong-Sung Lee7Hyuncheol Oh8Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, School of Pharmacy, Yantai University, Yantai 264005, ChinaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaResearch Institute of Pharmaceutical Sciences (RIPS), College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of KoreaDivision of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaDivision of Life Sciences, Korea Polar Research Institute, Incheon 21990, Republic of KoreaCollege of Medical and Life Sciences, Silla University, Busan 46958, Republic of KoreaResearch Institute of Pharmaceutical Sciences (RIPS), College of Pharmacy, Chosun University, Dong-gu, Gwangju 61452, Republic of KoreaInstitute of Pharmaceutical Research and Development, College of Pharmacy, Wonkwang University, Iksan 54538, Republic of KoreaInflammation has always been considered a trigger or consequence of neurodegenerative diseases, and the inhibition of inflammation in the central nervous system can effectively protect nerve cells. Several studies have indicated that various natural products inhibit neuroinflammation. Among these, Antarctic fungal metabolites have pharmacological activities and a developmental value. Therefore, this study aimed to evaluate the anti-neuroinflammatory activity of an Antarctic fungus belonging to <i>Aspergillus</i> (strain SF-7367). Secondary metabolites of SF-7367 were isolated using high-performance liquid chromatography followed by validation of their anti-inflammatory effects in lipopolysaccharide-stimulated BV2 microglia and RAW264.7 macrophages. Chemical analysis of metabolites from the fungal strain revealed five known compounds: epideoxybrevianamide E (<b>1</b>), brevianamide V/W (<b>2</b>), brevianamide K (<b>3</b>), brevianamide Q (<b>4</b>), and brevianamide R (<b>5</b>). Among these compounds, brevianamide K showed significant anti-inflammatory activity against both cell types. Results of Western blotting and molecular docking showed that brevianamide K could regulate the activation of nuclear factor kappa-light-chain-enhancer of activated B cell (NF-κB) signaling. This indicates that brevianamide K present in <i>Aspergillus</i> sp. (strain SF-7367) can inhibit inflammatory responses by reducing lipopolysaccharide-induced nuclear translocation of NF-κB (p65). These findings suggest that <i>Aspergillus</i> sp. (strain SF-7367) and brevianamide K are candidate agents for treating neurodegenerative diseases.https://www.mdpi.com/1420-3049/30/2/294<i>Antarctic fungi</i>prenylated indole alkaloidNF-κBanti-inflammationmolecular docking |
| spellingShingle | Zhiming Liu Chi-Su Yoon Thao Quyen Cao Hwan Lee Il-Chan Kim Joung Han Yim Jae Hak Sohn Dong-Sung Lee Hyuncheol Oh Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 Molecules <i>Antarctic fungi</i> prenylated indole alkaloid NF-κB anti-inflammation molecular docking |
| title | Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 |
| title_full | Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 |
| title_fullStr | Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 |
| title_full_unstemmed | Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 |
| title_short | Anti-Neuroinflammatory Effects of Prenylated Indole Alkaloids from the Antarctic Fungus <i>Aspergillus</i> sp. Strain SF-7367 |
| title_sort | anti neuroinflammatory effects of prenylated indole alkaloids from the antarctic fungus i aspergillus i sp strain sf 7367 |
| topic | <i>Antarctic fungi</i> prenylated indole alkaloid NF-κB anti-inflammation molecular docking |
| url | https://www.mdpi.com/1420-3049/30/2/294 |
| work_keys_str_mv | AT zhimingliu antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT chisuyoon antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT thaoquyencao antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT hwanlee antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT ilchankim antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT jounghanyim antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT jaehaksohn antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT dongsunglee antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 AT hyuncheoloh antineuroinflammatoryeffectsofprenylatedindolealkaloidsfromtheantarcticfungusiaspergillusispstrainsf7367 |