A real-time PCR method for rapid detection of Gymnodinium sanguineum
For detecting the harmful algal bloom (HAB) species sensitively and rapidly, Gymnodinium sanguineum was taken as the object, and the rapid detection method- RFQ-PCR (real-time fluorescent quantitative polymerase chain reaction) technique was applied to the HAB species detection. Firstly, gene specif...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2009-03-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2009.02.001 |
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| Summary: | For detecting the harmful algal bloom (HAB) species sensitively and rapidly, Gymnodinium sanguineum was taken as the object, and the rapid detection method- RFQ-PCR (real-time fluorescent quantitative polymerase chain reaction) technique was applied to the HAB species detection. Firstly, gene specific primers and a TaqMan DNA probe were designed based on the sequence of 18S rDNA cloned from G. sanguineum. Then, using the primers and probe combination, a RFQ-PCR method was developed for quantitative detection of G. sanguineum, a harmful algae bloom species. The RFQ-PCR assay data showed good agreement with traditional microscope counting methods, suggesting that RFQ-PCR may be a useful method for detecting G. sanguineum. The detection threshold tested was much lower than that of G. sanguineum HAB, so this technique will be useful for forecasting and preventing G. sanguineum HAB. |
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| ISSN: | 1008-9209 2097-5155 |