Protocol to study toxic, immune, and suicidal phenotypes of killer yeast
Summary: Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000176 |
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| author | Rianne C. Prins Sonja Billerbeck |
| author_facet | Rianne C. Prins Sonja Billerbeck |
| author_sort | Rianne C. Prins |
| collection | DOAJ |
| description | Summary: Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis.For complete details on the use and execution of this protocol, please refer to Prins et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-8366f0b54fdb486b8ce829ae5960f4e2 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-8366f0b54fdb486b8ce829ae5960f4e22025-02-02T05:29:21ZengElsevierSTAR Protocols2666-16672025-03-0161103611Protocol to study toxic, immune, and suicidal phenotypes of killer yeastRianne C. Prins0Sonja Billerbeck1Molecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, the NetherlandsMolecular Microbiology, Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Groningen 9747 AG, the Netherlands; Department of Bioengineering, Imperial College London, London SW7 2AZ, UK; Corresponding authorSummary: Killer yeast produce antimicrobial proteins, often together with a self-protective immunity factor. Here, we present a protocol for analyzing the toxic, immune, and suicidal phenotypes in Saccharomyces cerevisiae. We describe steps for assessing toxicity via halo assays, suicidality through spot assays, and immunity using microtiter plate growth assays. We detail procedures for yeast culturing, performing the assays, and data analysis.For complete details on the use and execution of this protocol, please refer to Prins et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000176microbiologymolecular biologybiotechnology and bioengineering |
| spellingShingle | Rianne C. Prins Sonja Billerbeck Protocol to study toxic, immune, and suicidal phenotypes of killer yeast STAR Protocols microbiology molecular biology biotechnology and bioengineering |
| title | Protocol to study toxic, immune, and suicidal phenotypes of killer yeast |
| title_full | Protocol to study toxic, immune, and suicidal phenotypes of killer yeast |
| title_fullStr | Protocol to study toxic, immune, and suicidal phenotypes of killer yeast |
| title_full_unstemmed | Protocol to study toxic, immune, and suicidal phenotypes of killer yeast |
| title_short | Protocol to study toxic, immune, and suicidal phenotypes of killer yeast |
| title_sort | protocol to study toxic immune and suicidal phenotypes of killer yeast |
| topic | microbiology molecular biology biotechnology and bioengineering |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000176 |
| work_keys_str_mv | AT riannecprins protocoltostudytoxicimmuneandsuicidalphenotypesofkilleryeast AT sonjabillerbeck protocoltostudytoxicimmuneandsuicidalphenotypesofkilleryeast |