Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride
Gemcitabine (2,2-difluorodeoxycytidine) is a deoxycytidine analog, currently being used as a first-choice drug in pancreatic metastatic cancer. Gemcitabine is administered weekly as 30-minute infusion with starting dose ranging from 800 to 1250 mg/m2. The aim of the present work was to develop starc...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/560962 |
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| author | Rekha Khaira Jyoti Sharma Vinay Saini |
| author_facet | Rekha Khaira Jyoti Sharma Vinay Saini |
| author_sort | Rekha Khaira |
| collection | DOAJ |
| description | Gemcitabine (2,2-difluorodeoxycytidine) is a deoxycytidine analog, currently being used as a first-choice drug in pancreatic metastatic cancer. Gemcitabine is administered weekly as 30-minute infusion with starting dose ranging from 800 to 1250 mg/m2. The aim of the present work was to develop starch nanoparticles (NPs) for the delivery of gemcitabine hydrochloride that could reduce its dose related side effects and may prolong its retention time (24 hrs) for the treatment of pancreatic cancer. Nanoparticles were prepared by emulsification diffusion method with slight modifications. Size and morphology of nanoparticles were investigated. Particles were spherical in shape with slightly rough surfaces. Particle size and polydispersity index were 231.4 nm and 1.0, respectively while zeta potential of blank NPs and drug loaded NPs were found to be −11.8 mV and −9.55 mV, respectively. Percent entrapment efficiency of different formulations was around ∼54% to 65%. In vitro release profile studies showed that around 70%–83% of drug was released from different formulations. Anticancerous cell line studies were also performed in human pancreatic cell lines (MIA-PA-CA-2). |
| format | Article |
| id | doaj-art-83616b2a9c0447ff9f05d3abf83c1f23 |
| institution | OA Journals |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
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| series | The Scientific World Journal |
| spelling | doaj-art-83616b2a9c0447ff9f05d3abf83c1f232025-08-20T02:09:01ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/560962560962Development and Characterization of Nanoparticles for the Delivery of Gemcitabine HydrochlorideRekha Khaira0Jyoti Sharma1Vinay Saini2Department of Pharmaceutics, SD College Pharmacy, Barnala Punjab 148101, IndiaDepartment of Pharmaceutics, SD College Pharmacy, Barnala Punjab 148101, IndiaDepartment of Pharmaceutics, SD College Pharmacy, Barnala Punjab 148101, IndiaGemcitabine (2,2-difluorodeoxycytidine) is a deoxycytidine analog, currently being used as a first-choice drug in pancreatic metastatic cancer. Gemcitabine is administered weekly as 30-minute infusion with starting dose ranging from 800 to 1250 mg/m2. The aim of the present work was to develop starch nanoparticles (NPs) for the delivery of gemcitabine hydrochloride that could reduce its dose related side effects and may prolong its retention time (24 hrs) for the treatment of pancreatic cancer. Nanoparticles were prepared by emulsification diffusion method with slight modifications. Size and morphology of nanoparticles were investigated. Particles were spherical in shape with slightly rough surfaces. Particle size and polydispersity index were 231.4 nm and 1.0, respectively while zeta potential of blank NPs and drug loaded NPs were found to be −11.8 mV and −9.55 mV, respectively. Percent entrapment efficiency of different formulations was around ∼54% to 65%. In vitro release profile studies showed that around 70%–83% of drug was released from different formulations. Anticancerous cell line studies were also performed in human pancreatic cell lines (MIA-PA-CA-2).http://dx.doi.org/10.1155/2014/560962 |
| spellingShingle | Rekha Khaira Jyoti Sharma Vinay Saini Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride The Scientific World Journal |
| title | Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride |
| title_full | Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride |
| title_fullStr | Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride |
| title_full_unstemmed | Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride |
| title_short | Development and Characterization of Nanoparticles for the Delivery of Gemcitabine Hydrochloride |
| title_sort | development and characterization of nanoparticles for the delivery of gemcitabine hydrochloride |
| url | http://dx.doi.org/10.1155/2014/560962 |
| work_keys_str_mv | AT rekhakhaira developmentandcharacterizationofnanoparticlesforthedeliveryofgemcitabinehydrochloride AT jyotisharma developmentandcharacterizationofnanoparticlesforthedeliveryofgemcitabinehydrochloride AT vinaysaini developmentandcharacterizationofnanoparticlesforthedeliveryofgemcitabinehydrochloride |