Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes
Visual impairment associated with uveitis is among the potential complications in multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Bioinformatics analyses have shown that some hub genes are closely associated with the molecular mechanisms underlying uvei...
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Taylor & Francis Group
2024-12-01
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| Series: | Animal Cells and Systems |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/19768354.2024.2304557 |
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| author | Sungmoo Hong Kyungsook Jung Meejung Ahn Jeongtae Kim Changjong Moon Taekyun Shin |
| author_facet | Sungmoo Hong Kyungsook Jung Meejung Ahn Jeongtae Kim Changjong Moon Taekyun Shin |
| author_sort | Sungmoo Hong |
| collection | DOAJ |
| description | Visual impairment associated with uveitis is among the potential complications in multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Bioinformatics analyses have shown that some hub genes are closely associated with the molecular mechanisms underlying uveitis in EAE. This study evaluated whether 4-allyl-2-methoxyphenol (eugenol) can mitigate the pathogenesis of uveitis in EAE through the interruption of key uveitogenic gene expression. Myelin oligodendrocyte glycoprotein35–55 (MOG) peptide-immunized C57BL/6 mice were injected intraperitoneally with eugenol. The eyeballs and spinal cords of EAE mice with or without eugenol treatment were collected simultaneously and immunohistochemical and molecular biological analyses were conducted. Eugenol treatment significantly ameliorated hindlimb paralysis. Ionized calcium-binding adapter molecule 1 (Iba-1) immunohistochemistry showed that the inflammatory response was significantly reduced in the uvea of eugenol-treated EAE mice compared with vehicle-treated controls. Eugenol also significantly reduced the expression of key uveitogenic genes including C1qb and Tyrobp. The suppressive effect of eugenol on inflammation was also observed in the spinal cord, as determined by the suppression of Iba-1-positive microglial cells. Together, these results suggest that the ameliorative effect of eugenol against EAE uveitis is associated with the suppression of key proinflammatory genes, which may represent targets for the treatment of uveitis. |
| format | Article |
| id | doaj-art-834f0768d7af46efa57c232f77e71c4c |
| institution | OA Journals |
| issn | 1976-8354 2151-2485 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
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| series | Animal Cells and Systems |
| spelling | doaj-art-834f0768d7af46efa57c232f77e71c4c2025-08-20T02:16:33ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852024-12-01281374410.1080/19768354.2024.2304557Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genesSungmoo Hong0Kyungsook Jung1Meejung Ahn2Jeongtae Kim3Changjong Moon4Taekyun Shin5College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Republic of KoreaFunctional Biomaterials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup-si, Republic of KoreaDepartment of Animal Science, College of Life Science, Sangji University, Wonju, Republic of KoreaDepartment of Anatomy, Kosin University College of Medicine, Busan, Republic of KoreaDepartment of Veterinary Anatomy and Animal Behavior, College of Veterinary Medicine and BK21 Plus Project Team, Chonnam National University, Gwangju, Republic of KoreaCollege of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University, Jeju, Republic of KoreaVisual impairment associated with uveitis is among the potential complications in multiple sclerosis (MS) and its animal model, experimental autoimmune encephalomyelitis (EAE). Bioinformatics analyses have shown that some hub genes are closely associated with the molecular mechanisms underlying uveitis in EAE. This study evaluated whether 4-allyl-2-methoxyphenol (eugenol) can mitigate the pathogenesis of uveitis in EAE through the interruption of key uveitogenic gene expression. Myelin oligodendrocyte glycoprotein35–55 (MOG) peptide-immunized C57BL/6 mice were injected intraperitoneally with eugenol. The eyeballs and spinal cords of EAE mice with or without eugenol treatment were collected simultaneously and immunohistochemical and molecular biological analyses were conducted. Eugenol treatment significantly ameliorated hindlimb paralysis. Ionized calcium-binding adapter molecule 1 (Iba-1) immunohistochemistry showed that the inflammatory response was significantly reduced in the uvea of eugenol-treated EAE mice compared with vehicle-treated controls. Eugenol also significantly reduced the expression of key uveitogenic genes including C1qb and Tyrobp. The suppressive effect of eugenol on inflammation was also observed in the spinal cord, as determined by the suppression of Iba-1-positive microglial cells. Together, these results suggest that the ameliorative effect of eugenol against EAE uveitis is associated with the suppression of key proinflammatory genes, which may represent targets for the treatment of uveitis.https://www.tandfonline.com/doi/10.1080/19768354.2024.2304557Anti-inflammationeugenolexperimental autoimmune encephalomyelitisneuroinflammationuveitis |
| spellingShingle | Sungmoo Hong Kyungsook Jung Meejung Ahn Jeongtae Kim Changjong Moon Taekyun Shin Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes Animal Cells and Systems Anti-inflammation eugenol experimental autoimmune encephalomyelitis neuroinflammation uveitis |
| title | Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| title_full | Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| title_fullStr | Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| title_full_unstemmed | Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| title_short | Eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| title_sort | eugenol ameliorates uveitis in mice with experimental autoimmune encephalomyelitis through the suppression of key inflammatory genes |
| topic | Anti-inflammation eugenol experimental autoimmune encephalomyelitis neuroinflammation uveitis |
| url | https://www.tandfonline.com/doi/10.1080/19768354.2024.2304557 |
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