Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum
A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. Samples were pretreated by a single-step precipitation with acetonitrile....
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2020-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/8838219 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551261713465344 |
|---|---|
| author | Yunliang Zheng Nana Xu Xingjiang Hu Qiao Zhang Yanpeng Liu Qingwei Zhao |
| author_facet | Yunliang Zheng Nana Xu Xingjiang Hu Qiao Zhang Yanpeng Liu Qingwei Zhao |
| author_sort | Yunliang Zheng |
| collection | DOAJ |
| description | A simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. Samples were pretreated by a single-step precipitation with acetonitrile. Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min. MS detection was performed by a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive electrospray ionization source. Isotope-labeled internal standard, especially rifampicin-D8, was applied to adjust for the loss during sample treatment. The established LC-MS/MS method showed a wide analytical range (pyrazinamide: 1.02∼60.0 μg/mL, isoniazid: 0.152∼10.0 μg/mL, rifampicin: 0.500∼30.0 μg/mL, and ethambutol: 0.0998∼5.99 μg/mL) and a good linearity (r > 0.99 for the four analytes) with acceptable accuracy and precision (90.15%∼104.62% and 94.00%∼104.02% for intra- and interaccuracy, respectively; RSD%: <12.46% and <6.43% for intra- and interprecision, respectively). It also showed excellent recoveries (79.24%∼94.16% for all analytes) and absence of significant matrix effect. This method was successfully applied to the quantification of four first-line antituberculosis (anti-TB) drugs, suggesting its suitability for therapeutic drug monitoring in the clinical practices. |
| format | Article |
| id | doaj-art-8339b073df2e429f941516d45bdf11cf |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-8339b073df2e429f941516d45bdf11cf2025-02-03T06:04:35ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/88382198838219Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human SerumYunliang Zheng0Nana Xu1Xingjiang Hu2Qiao Zhang3Yanpeng Liu4Qingwei Zhao5Zhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaZhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaZhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaZhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaZhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaZhejiang Provincial Key Laboratory for Drug Evaluation and Clinical Research, The First Affiliated Hospital of Zhejiang University, School of Medicine, 79 Qingchun Road, Hangzhou, Zhejiang 310003, ChinaA simple, rapid, and sensitive liquid chromatography (LC)/mass spectrometry (MS) method was established and validated for simultaneous quantitation of pyrazinamide, isoniazid, rifampicin, and ethambutol in human blood sample. Samples were pretreated by a single-step precipitation with acetonitrile. Chromatographic separation was achieved on XSelecT HSS T3 column by gradient elution with a total run time of 5.0 min. MS detection was performed by a triple quadrupole tandem mass spectrometer in the multiple reaction monitoring mode with a positive electrospray ionization source. Isotope-labeled internal standard, especially rifampicin-D8, was applied to adjust for the loss during sample treatment. The established LC-MS/MS method showed a wide analytical range (pyrazinamide: 1.02∼60.0 μg/mL, isoniazid: 0.152∼10.0 μg/mL, rifampicin: 0.500∼30.0 μg/mL, and ethambutol: 0.0998∼5.99 μg/mL) and a good linearity (r > 0.99 for the four analytes) with acceptable accuracy and precision (90.15%∼104.62% and 94.00%∼104.02% for intra- and interaccuracy, respectively; RSD%: <12.46% and <6.43% for intra- and interprecision, respectively). It also showed excellent recoveries (79.24%∼94.16% for all analytes) and absence of significant matrix effect. This method was successfully applied to the quantification of four first-line antituberculosis (anti-TB) drugs, suggesting its suitability for therapeutic drug monitoring in the clinical practices.http://dx.doi.org/10.1155/2020/8838219 |
| spellingShingle | Yunliang Zheng Nana Xu Xingjiang Hu Qiao Zhang Yanpeng Liu Qingwei Zhao Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum Journal of Analytical Methods in Chemistry |
| title | Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum |
| title_full | Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum |
| title_fullStr | Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum |
| title_full_unstemmed | Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum |
| title_short | Development and Application of a LC-MS/MS Method for Simultaneous Quantification of Four First-Line Antituberculosis Drugs in Human Serum |
| title_sort | development and application of a lc ms ms method for simultaneous quantification of four first line antituberculosis drugs in human serum |
| url | http://dx.doi.org/10.1155/2020/8838219 |
| work_keys_str_mv | AT yunliangzheng developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum AT nanaxu developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum AT xingjianghu developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum AT qiaozhang developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum AT yanpengliu developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum AT qingweizhao developmentandapplicationofalcmsmsmethodforsimultaneousquantificationoffourfirstlineantituberculosisdrugsinhumanserum |