Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis
In 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly wi...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Veterinary Medicine International |
| Online Access: | http://dx.doi.org/10.4061/2011/953985 |
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| author | W. Ray Waters Gary E. Stevens Mark A. Schoenbaum Kathy A. Orloski Suelee Robbe-Austerman N. Beth Harris S. Mark Hall Bruce V. Thomsen Arach J. Wilson Roger E. Brannian Jeffrey T. Nelson Shawn Schafer Javan Esfandiari Meghan Dutton Rena Greenwald Konstantin P. Lyashchenko |
| author_facet | W. Ray Waters Gary E. Stevens Mark A. Schoenbaum Kathy A. Orloski Suelee Robbe-Austerman N. Beth Harris S. Mark Hall Bruce V. Thomsen Arach J. Wilson Roger E. Brannian Jeffrey T. Nelson Shawn Schafer Javan Esfandiari Meghan Dutton Rena Greenwald Konstantin P. Lyashchenko |
| author_sort | W. Ray Waters |
| collection | DOAJ |
| description | In 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly with elk, gross lesions were often severe and extensive. One year ago, the majority of the elk had been tested for TB by single cervical test (SCT), and all were negative. After initial detection of a tuberculous elk in this herd, 42 of the 59 elk were tested by SCT. Of the 42 SCT-tested elk, 28 were TB-infected with only 3/28 reacting upon SCT. After SCT, serum samples were collected from the infected elk and fallow deer from this herd at necropsy and tested by three antibody detection methods including multiantigen print immunoassay, cervidTB STAT-PAK, and dual path platform VetTB (DPP). Serologic test sensitivity ranged from 79 to 97% depending on the test format and host species. Together, these findings demonstrate the opportunities for use of serodiagnosis in the rapid detection of TB in elk and fallow deer. |
| format | Article |
| id | doaj-art-832fbd1644de4e59873c3044d172863b |
| institution | Kabale University |
| issn | 2042-0048 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine International |
| spelling | doaj-art-832fbd1644de4e59873c3044d172863b2025-02-03T01:23:34ZengWileyVeterinary Medicine International2042-00482011-01-01201110.4061/2011/953985953985Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for SerodiagnosisW. Ray Waters0Gary E. Stevens1Mark A. Schoenbaum2Kathy A. Orloski3Suelee Robbe-Austerman4N. Beth Harris5S. Mark Hall6Bruce V. Thomsen7Arach J. Wilson8Roger E. Brannian9Jeffrey T. Nelson10Shawn Schafer11Javan Esfandiari12Meghan Dutton13Rena Greenwald14Konstantin P. Lyashchenko15National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture (USDA), Ames, IA 50010, USANational Veterinary Services Laboratories (NVSL), Animal and Plant Health Inspection Service (APHIS), USDA, Lincoln, NE 68516, USANVSL, APHIS, USDA, Fort Collins, CO 80526-8117, USANVSL, APHIS, USDA, Fort Collins, CO 80526-8117, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANVSL, APHIS, USDA, Ames, IA 50010, USANorth American Deer Farmers Association, Lake City, MN 55041, USAChembio Diagnostic Systems, Inc., Medford, NY 11763, USAChembio Diagnostic Systems, Inc., Medford, NY 11763, USAChembio Diagnostic Systems, Inc., Medford, NY 11763, USAChembio Diagnostic Systems, Inc., Medford, NY 11763, USAIn 2009, Mycobacterium bovis infection was detected in a herd of 60 elk (Cervus elaphus) and 50 fallow deer (Dama dama) in Nebraska, USA. Upon depopulation of the herd, the prevalence of bovine tuberculosis (TB) was estimated at ∼71–75%, based upon histopathology and culture results. Particularly with elk, gross lesions were often severe and extensive. One year ago, the majority of the elk had been tested for TB by single cervical test (SCT), and all were negative. After initial detection of a tuberculous elk in this herd, 42 of the 59 elk were tested by SCT. Of the 42 SCT-tested elk, 28 were TB-infected with only 3/28 reacting upon SCT. After SCT, serum samples were collected from the infected elk and fallow deer from this herd at necropsy and tested by three antibody detection methods including multiantigen print immunoassay, cervidTB STAT-PAK, and dual path platform VetTB (DPP). Serologic test sensitivity ranged from 79 to 97% depending on the test format and host species. Together, these findings demonstrate the opportunities for use of serodiagnosis in the rapid detection of TB in elk and fallow deer.http://dx.doi.org/10.4061/2011/953985 |
| spellingShingle | W. Ray Waters Gary E. Stevens Mark A. Schoenbaum Kathy A. Orloski Suelee Robbe-Austerman N. Beth Harris S. Mark Hall Bruce V. Thomsen Arach J. Wilson Roger E. Brannian Jeffrey T. Nelson Shawn Schafer Javan Esfandiari Meghan Dutton Rena Greenwald Konstantin P. Lyashchenko Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis Veterinary Medicine International |
| title | Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis |
| title_full | Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis |
| title_fullStr | Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis |
| title_full_unstemmed | Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis |
| title_short | Bovine Tuberculosis in a Nebraska Herd of Farmed Elk and Fallow Deer: A Failure of the Tuberculin Skin Test and Opportunities for Serodiagnosis |
| title_sort | bovine tuberculosis in a nebraska herd of farmed elk and fallow deer a failure of the tuberculin skin test and opportunities for serodiagnosis |
| url | http://dx.doi.org/10.4061/2011/953985 |
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