Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro
Endometriosis is an inflammatory disease marked by ectopic growth of endometrial cells. Mesenchymal stromal cells (MSC) have immunosuppressive properties that have been suggested as a treatment for inflammatory diseases. Therefore, the aim herein was to examine effects of allogeneic MSC on endometri...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2018/7318513 |
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| _version_ | 1832567108686315520 |
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| author | Fawaz Abomaray Sebastian Gidlöf Bartosz Bezubik Mikael Engman Cecilia Götherström |
| author_facet | Fawaz Abomaray Sebastian Gidlöf Bartosz Bezubik Mikael Engman Cecilia Götherström |
| author_sort | Fawaz Abomaray |
| collection | DOAJ |
| description | Endometriosis is an inflammatory disease marked by ectopic growth of endometrial cells. Mesenchymal stromal cells (MSC) have immunosuppressive properties that have been suggested as a treatment for inflammatory diseases. Therefore, the aim herein was to examine effects of allogeneic MSC on endometriosis-derived cells in vitro as a potential therapy for endometriosis. MSC from allogeneic adipose tissue (Ad-MSC) and stromal cells from endometrium (ESCendo) and endometriotic ovarian cysts (ESCcyst) from women with endometriosis were isolated. The effects of Ad-MSC on ESCendo and ESCcyst were investigated using in vitro proliferation, apoptosis, adhesion, tube formation, migration, and invasion assays. Ad-MSC significantly increased proliferation of ESC compared to untreated controls. Moreover, Ad-MSC significantly decreased apoptosis and increased survival of ESC. Ad-MSC significantly increased adhesion of ESCendo and not ESCcyst on fibronectin. Conditioned medium from cocultures of Ad-MSC and ESC significantly increased tube formation of human umbilical vein endothelial cells on matrigel. Ad-MSC may significantly increase migration of ESCcyst and did not increase invasion of both cell types. The data suggest that allogeneic Ad-MSC should not be considered as a potential therapy for endometriosis, because they may support the pathology by maintaining and increasing growth of ectopic endometrial tissue. |
| format | Article |
| id | doaj-art-8314168f35b5423080e8b8059d711982 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-8314168f35b5423080e8b8059d7119822025-02-03T01:02:25ZengWileyStem Cells International1687-966X1687-96782018-01-01201810.1155/2018/73185137318513Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In VitroFawaz Abomaray0Sebastian Gidlöf1Bartosz Bezubik2Mikael Engman3Cecilia Götherström4Division of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenDivision of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenDepartment of Obstetrics and Gynecology, Karolinska University Hospital, Stockholm, SwedenDepartment of Obstetrics and Gynecology, Danderyd’s Hospital, Stockholm, SwedenDivision of Obstetrics and Gynecology, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, SwedenEndometriosis is an inflammatory disease marked by ectopic growth of endometrial cells. Mesenchymal stromal cells (MSC) have immunosuppressive properties that have been suggested as a treatment for inflammatory diseases. Therefore, the aim herein was to examine effects of allogeneic MSC on endometriosis-derived cells in vitro as a potential therapy for endometriosis. MSC from allogeneic adipose tissue (Ad-MSC) and stromal cells from endometrium (ESCendo) and endometriotic ovarian cysts (ESCcyst) from women with endometriosis were isolated. The effects of Ad-MSC on ESCendo and ESCcyst were investigated using in vitro proliferation, apoptosis, adhesion, tube formation, migration, and invasion assays. Ad-MSC significantly increased proliferation of ESC compared to untreated controls. Moreover, Ad-MSC significantly decreased apoptosis and increased survival of ESC. Ad-MSC significantly increased adhesion of ESCendo and not ESCcyst on fibronectin. Conditioned medium from cocultures of Ad-MSC and ESC significantly increased tube formation of human umbilical vein endothelial cells on matrigel. Ad-MSC may significantly increase migration of ESCcyst and did not increase invasion of both cell types. The data suggest that allogeneic Ad-MSC should not be considered as a potential therapy for endometriosis, because they may support the pathology by maintaining and increasing growth of ectopic endometrial tissue.http://dx.doi.org/10.1155/2018/7318513 |
| spellingShingle | Fawaz Abomaray Sebastian Gidlöf Bartosz Bezubik Mikael Engman Cecilia Götherström Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro Stem Cells International |
| title | Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro |
| title_full | Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro |
| title_fullStr | Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro |
| title_full_unstemmed | Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro |
| title_short | Mesenchymal Stromal Cells Support Endometriotic Stromal Cells In Vitro |
| title_sort | mesenchymal stromal cells support endometriotic stromal cells in vitro |
| url | http://dx.doi.org/10.1155/2018/7318513 |
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