Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland
The Pseudomonas genus includes species present in various environments and known for antibiotic resistance. However, only hospital-associated Pseudomonas aeruginosa have been extensively studied regarding antibiotic resistance. Thus, to fill the gap in knowledge on antibiotic resistance among other...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454510/full |
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| author | Wioleta Lewandowska Wioleta Lewandowska Jacques Mahillon Justyna Małgorzata Drewnowska Izabela Swiecicka Izabela Swiecicka |
| author_facet | Wioleta Lewandowska Wioleta Lewandowska Jacques Mahillon Justyna Małgorzata Drewnowska Izabela Swiecicka Izabela Swiecicka |
| author_sort | Wioleta Lewandowska |
| collection | DOAJ |
| description | The Pseudomonas genus includes species present in various environments and known for antibiotic resistance. However, only hospital-associated Pseudomonas aeruginosa have been extensively studied regarding antibiotic resistance. Thus, to fill the gap in knowledge on antibiotic resistance among other Pseudomonas spp., we investigated 41 isolates from soil samples taken in the Białowieża National Park in Northeastern Poland. This unique forest without notable anthropogenic influence, provides excellent conditions for research of antibiotic resistance from the perspective of natural environments. The phylogeny trees obtained based on the nucleotide sequence of the 16S rRNA gene and gyrB gene grouped the isolates into clusters belonging to the Pseudomonas fluorescens, Pseudomonas koreensis, and Pseudomonas putida groups, originating from the P. fluorescens lineage. All isolates under study demonstrated resistance to at least 12 out of the 24 antibiotics tested. Resistance to colistin, cefotaxime, and imipenem was detected in 73, 73, and 17% of the isolates, respectively. Most isolates showing resistance to imipenem and colistin clustered within the P. fluorescens group. Seven isolates were highly multi-resistant, to up to 18 of the 24 antibiotics tested. The presence of resistance genes related to intrinsic resistance of P. aeruginosa has been confirmed in environmental isolates. |
| format | Article |
| id | doaj-art-8304d8a41bb2404c9c6db259887020c5 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj-art-8304d8a41bb2404c9c6db259887020c52025-01-22T07:11:30ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-01-011610.3389/fmicb.2025.14545101454510Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern PolandWioleta Lewandowska0Wioleta Lewandowska1Jacques Mahillon2Justyna Małgorzata Drewnowska3Izabela Swiecicka4Izabela Swiecicka5Doctoral School, University of Białystok, Białystok, PolandDepartment of Microbiology and Biotechnology, Faculty of Biology, University of Białystok, Białystok, PolandLaboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, BelgiumLaboratory of Applied Microbiology, Faculty of Biology, University of Białystok, Białystok, PolandDepartment of Microbiology and Biotechnology, Faculty of Biology, University of Białystok, Białystok, PolandLaboratory of Applied Microbiology, Faculty of Biology, University of Białystok, Białystok, PolandThe Pseudomonas genus includes species present in various environments and known for antibiotic resistance. However, only hospital-associated Pseudomonas aeruginosa have been extensively studied regarding antibiotic resistance. Thus, to fill the gap in knowledge on antibiotic resistance among other Pseudomonas spp., we investigated 41 isolates from soil samples taken in the Białowieża National Park in Northeastern Poland. This unique forest without notable anthropogenic influence, provides excellent conditions for research of antibiotic resistance from the perspective of natural environments. The phylogeny trees obtained based on the nucleotide sequence of the 16S rRNA gene and gyrB gene grouped the isolates into clusters belonging to the Pseudomonas fluorescens, Pseudomonas koreensis, and Pseudomonas putida groups, originating from the P. fluorescens lineage. All isolates under study demonstrated resistance to at least 12 out of the 24 antibiotics tested. Resistance to colistin, cefotaxime, and imipenem was detected in 73, 73, and 17% of the isolates, respectively. Most isolates showing resistance to imipenem and colistin clustered within the P. fluorescens group. Seven isolates were highly multi-resistant, to up to 18 of the 24 antibiotics tested. The presence of resistance genes related to intrinsic resistance of P. aeruginosa has been confirmed in environmental isolates.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454510/fullPseudomonas spp.environmental isolatesBiałowieża National Parkphylogenyantibiotic resistancesoil |
| spellingShingle | Wioleta Lewandowska Wioleta Lewandowska Jacques Mahillon Justyna Małgorzata Drewnowska Izabela Swiecicka Izabela Swiecicka Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland Frontiers in Microbiology Pseudomonas spp. environmental isolates Białowieża National Park phylogeny antibiotic resistance soil |
| title | Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland |
| title_full | Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland |
| title_fullStr | Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland |
| title_full_unstemmed | Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland |
| title_short | Insight into the phylogeny and antibiotic resistance of Pseudomonas spp. originating from soil of the Białowieża National Park in Northeastern Poland |
| title_sort | insight into the phylogeny and antibiotic resistance of pseudomonas spp originating from soil of the bialowieza national park in northeastern poland |
| topic | Pseudomonas spp. environmental isolates Białowieża National Park phylogeny antibiotic resistance soil |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1454510/full |
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