Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice
Objectives. The aim of this study was to investigate the effect of nitric oxide (NO) on orthodontic tooth movement and the regulatory effect on bone formation. Design. A mouse orthodontic tooth movement model was established to measure the level of releasing NO. Besides, orthodontic tooth movement d...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2022/4775445 |
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| _version_ | 1832555187259047936 |
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| author | Yuqing Sun Jingfei Fu Feiran Lin Shengnan Li Juan Du Yi Liu Yuxing Bai |
| author_facet | Yuqing Sun Jingfei Fu Feiran Lin Shengnan Li Juan Du Yi Liu Yuxing Bai |
| author_sort | Yuqing Sun |
| collection | DOAJ |
| description | Objectives. The aim of this study was to investigate the effect of nitric oxide (NO) on orthodontic tooth movement and the regulatory effect on bone formation. Design. A mouse orthodontic tooth movement model was established to measure the level of releasing NO. Besides, orthodontic tooth movement distance and the bone formation in the tension side of the orthodontic tooth were also analyzed. In vitro, human periodontal ligament stem cells (hPDLSCs) were cultured under tensile force stimulation. The production of NO and the expression level of nitric oxide synthase (NOS) were detected after mechanical stimulation. Furthermore, the downstream cellular signaling pathway regulated by NO was also explored. Results. The generation of NO steadily increased throughout the orthodontic tooth movement in mice. Orthodontic tooth movement was decreased in the NOS inhibitor group while it was accelerated in the NO precursor group. Force-induced NO promoted the osteogenic differentiation of human hPDLSCs under tensile force stimulation. And force-induced NO in hPDLSCs regulated the PI3K/Akt/β-catenin signal pathway. Conclusion. NO is involved in the regulation of orthodontic tooth movement and promotes bone formation on the tension side of the orthodontic tooth. The PI3K/Akt/β-catenin pathway is one of the downstream cell signal transduction pathways of NO in the orthodontic process. |
| format | Article |
| id | doaj-art-82f6cc62c73242cc95b8c4256eeacbc5 |
| institution | Kabale University |
| issn | 1687-9678 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-82f6cc62c73242cc95b8c4256eeacbc52025-02-03T05:49:21ZengWileyStem Cells International1687-96782022-01-01202210.1155/2022/4775445Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in MiceYuqing Sun0Jingfei Fu1Feiran Lin2Shengnan Li3Juan Du4Yi Liu5Yuxing Bai6Department of OrthodonticsLaboratory of Tissue Regeneration and Immunology and Department of PeriodonticsLaboratory of Tissue Regeneration and Immunology and Department of PeriodonticsDepartment of OrthodonticsLaboratory of Tissue Regeneration and Immunology and Department of PeriodonticsLaboratory of Tissue Regeneration and Immunology and Department of PeriodonticsDepartment of OrthodonticsObjectives. The aim of this study was to investigate the effect of nitric oxide (NO) on orthodontic tooth movement and the regulatory effect on bone formation. Design. A mouse orthodontic tooth movement model was established to measure the level of releasing NO. Besides, orthodontic tooth movement distance and the bone formation in the tension side of the orthodontic tooth were also analyzed. In vitro, human periodontal ligament stem cells (hPDLSCs) were cultured under tensile force stimulation. The production of NO and the expression level of nitric oxide synthase (NOS) were detected after mechanical stimulation. Furthermore, the downstream cellular signaling pathway regulated by NO was also explored. Results. The generation of NO steadily increased throughout the orthodontic tooth movement in mice. Orthodontic tooth movement was decreased in the NOS inhibitor group while it was accelerated in the NO precursor group. Force-induced NO promoted the osteogenic differentiation of human hPDLSCs under tensile force stimulation. And force-induced NO in hPDLSCs regulated the PI3K/Akt/β-catenin signal pathway. Conclusion. NO is involved in the regulation of orthodontic tooth movement and promotes bone formation on the tension side of the orthodontic tooth. The PI3K/Akt/β-catenin pathway is one of the downstream cell signal transduction pathways of NO in the orthodontic process.http://dx.doi.org/10.1155/2022/4775445 |
| spellingShingle | Yuqing Sun Jingfei Fu Feiran Lin Shengnan Li Juan Du Yi Liu Yuxing Bai Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice Stem Cells International |
| title | Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice |
| title_full | Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice |
| title_fullStr | Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice |
| title_full_unstemmed | Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice |
| title_short | Force-Induced Nitric Oxide Promotes Osteogenic Activity during Orthodontic Tooth Movement in Mice |
| title_sort | force induced nitric oxide promotes osteogenic activity during orthodontic tooth movement in mice |
| url | http://dx.doi.org/10.1155/2022/4775445 |
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