Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer
Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) is a morphogen in an in vitro model for lumen formation and plays a similar role in breast epithelial cells implanted in humanized mammary fat pads in NOD-SCID mice. Although extra cellular matrix alone is sufficient to stimulate l...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | International Journal of Breast Cancer |
| Online Access: | http://dx.doi.org/10.4061/2011/381080 |
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| author | Sridhar Samineni Carlotta Glackin John E. Shively |
| author_facet | Sridhar Samineni Carlotta Glackin John E. Shively |
| author_sort | Sridhar Samineni |
| collection | DOAJ |
| description | Carcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) is a morphogen in an in vitro model for lumen formation and plays a similar role in breast epithelial cells implanted in humanized mammary fat pads in NOD-SCID mice. Although extra cellular matrix alone is sufficient to stimulate lumen formation in CEACAM1 transfected MCF-7 cells grown in 3D culture, there is an additional requirement for stromal or mesenchymal cells (MSCs) for these cells to form xenografts with glandular structures in an orthotopic site. We demonstrate that optimal in vitro conditions include both Matrigel and MSCs and that the inclusion of collagen I inhibits xenograft differentiation. Additionally, there is no need to remove the nascent murine mammary gland. The previously observed difference in gland development between the long and short cytoplasmic domain isoforms of CEACAM1 is no longer observed in pregnant NOD/SCID mice suggesting that stimulation of the mammary fat pad by pregnancy critically affects xenograft differentiation. |
| format | Article |
| id | doaj-art-82f2a2760fb5488ca25b6f22c87c4c75 |
| institution | Kabale University |
| issn | 2090-3189 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Breast Cancer |
| spelling | doaj-art-82f2a2760fb5488ca25b6f22c87c4c752025-02-03T05:53:44ZengWileyInternational Journal of Breast Cancer2090-31892011-01-01201110.4061/2011/381080381080Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast CancerSridhar Samineni0Carlotta Glackin1John E. Shively2Irell & Manella City of Hope Graduate School of Biological Sciences, Duarte, CA 91010, USADepartment of Neurosciences, Beckman Research Institute of City of Hope, Duarte, CA 91010, USADepartment of Immunology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USACarcinoembryonic antigen-related cell adhesion molecule-1 (CEACAM1) is a morphogen in an in vitro model for lumen formation and plays a similar role in breast epithelial cells implanted in humanized mammary fat pads in NOD-SCID mice. Although extra cellular matrix alone is sufficient to stimulate lumen formation in CEACAM1 transfected MCF-7 cells grown in 3D culture, there is an additional requirement for stromal or mesenchymal cells (MSCs) for these cells to form xenografts with glandular structures in an orthotopic site. We demonstrate that optimal in vitro conditions include both Matrigel and MSCs and that the inclusion of collagen I inhibits xenograft differentiation. Additionally, there is no need to remove the nascent murine mammary gland. The previously observed difference in gland development between the long and short cytoplasmic domain isoforms of CEACAM1 is no longer observed in pregnant NOD/SCID mice suggesting that stimulation of the mammary fat pad by pregnancy critically affects xenograft differentiation.http://dx.doi.org/10.4061/2011/381080 |
| spellingShingle | Sridhar Samineni Carlotta Glackin John E. Shively Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer International Journal of Breast Cancer |
| title | Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer |
| title_full | Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer |
| title_fullStr | Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer |
| title_full_unstemmed | Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer |
| title_short | Role of CEACAM1, ECM, and Mesenchymal Stem Cells in an Orthotopic Model of Human Breast Cancer |
| title_sort | role of ceacam1 ecm and mesenchymal stem cells in an orthotopic model of human breast cancer |
| url | http://dx.doi.org/10.4061/2011/381080 |
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