Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis
This study aims to determine whether the combined blockade of IL-1β and TNF-α can alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR gu...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2016/3128182 |
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| author | Hu Wei-xu Zhou Wen-yun Zhu Xi-ling Wen Zhu Wu Li-hua Wu Xiao-mu Wei Hui-ping Wang Wen-ding He Dan Xiang Qin Hu Guo-zhu |
| author_facet | Hu Wei-xu Zhou Wen-yun Zhu Xi-ling Wen Zhu Wu Li-hua Wu Xiao-mu Wei Hui-ping Wang Wen-ding He Dan Xiang Qin Hu Guo-zhu |
| author_sort | Hu Wei-xu |
| collection | DOAJ |
| description | This study aims to determine whether the combined blockade of IL-1β and TNF-α can alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR guinea pigs were randomly divided into (1) the AR model group treated with intranasal saline; (2) the 0.1% nonspecific IgY treatment group; (3) the 0.1% anti-TNF-α IgY treatment group; (4) the 0.1% anti-IL-1β IgY treatment group; (5) the 0.1% combined anti-IL-1β and TNF-α IgY treatment group; and (6) the fluticasone propionate treatment group. The inflammatory cells were evaluated using Wright’s staining. Histopathology was examined using hematoxylin-eosin staining. The results showed that the number of eosinophils was significantly decreased in the peripheral blood, nasal lavage fluid, and bronchoalveolar lavage fluid (P<0.05), and eosinophil, neutrophil, and lymphocyte infiltration and edema were significantly reduced or absent in the nasal mucosa and lung tissues (P<0.05) in the combined 0.1% anti-IL-1β- and TNF-α IgY-treated guinea pigs. The data suggest that topical blockade of IL-1β and TNF-α could reduce pathological allergic inflammation in the nasal mucosa and lung tissues in AR guinea pigs. |
| format | Article |
| id | doaj-art-82ec37476850413ea344ae727eb0f00b |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-82ec37476850413ea344ae727eb0f00b2025-02-03T06:00:05ZengWileyMediators of Inflammation0962-93511466-18612016-01-01201610.1155/2016/31281823128182Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic RhinitisHu Wei-xu0Zhou Wen-yun1Zhu Xi-ling2Wen Zhu3Wu Li-hua4Wu Xiao-mu5Wei Hui-ping6Wang Wen-ding7He Dan8Xiang Qin9Hu Guo-zhu10Department of Radiation Oncology, Shanghai Proton and Heavy Ion Center, Shanghai 201315, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaDepartment of Hematology, Jiangxi Academy of Medical Science, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaState Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, Hunan 410082, ChinaInstitute of Clinical Medical Sciences, Jiangxi Province People’s Hospital, Nanchang, Jiangxi 330006, ChinaThis study aims to determine whether the combined blockade of IL-1β and TNF-α can alleviate the pathological allergic inflammatory reaction in the nasal mucosa and lung tissues in allergic rhinitis (AR) guinea pigs. Healthy guinea pigs treated with saline were used as the healthy controls. The AR guinea pigs were randomly divided into (1) the AR model group treated with intranasal saline; (2) the 0.1% nonspecific IgY treatment group; (3) the 0.1% anti-TNF-α IgY treatment group; (4) the 0.1% anti-IL-1β IgY treatment group; (5) the 0.1% combined anti-IL-1β and TNF-α IgY treatment group; and (6) the fluticasone propionate treatment group. The inflammatory cells were evaluated using Wright’s staining. Histopathology was examined using hematoxylin-eosin staining. The results showed that the number of eosinophils was significantly decreased in the peripheral blood, nasal lavage fluid, and bronchoalveolar lavage fluid (P<0.05), and eosinophil, neutrophil, and lymphocyte infiltration and edema were significantly reduced or absent in the nasal mucosa and lung tissues (P<0.05) in the combined 0.1% anti-IL-1β- and TNF-α IgY-treated guinea pigs. The data suggest that topical blockade of IL-1β and TNF-α could reduce pathological allergic inflammation in the nasal mucosa and lung tissues in AR guinea pigs.http://dx.doi.org/10.1155/2016/3128182 |
| spellingShingle | Hu Wei-xu Zhou Wen-yun Zhu Xi-ling Wen Zhu Wu Li-hua Wu Xiao-mu Wei Hui-ping Wang Wen-ding He Dan Xiang Qin Hu Guo-zhu Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis Mediators of Inflammation |
| title | Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis |
| title_full | Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis |
| title_fullStr | Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis |
| title_full_unstemmed | Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis |
| title_short | Anti-Interleukin-1 Beta/Tumor Necrosis Factor-Alpha IgY Antibodies Reduce Pathological Allergic Responses in Guinea Pigs with Allergic Rhinitis |
| title_sort | anti interleukin 1 beta tumor necrosis factor alpha igy antibodies reduce pathological allergic responses in guinea pigs with allergic rhinitis |
| url | http://dx.doi.org/10.1155/2016/3128182 |
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