Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC
The pharmacodynamic effect of longan leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C1), a...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2020/3950609 |
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| author | Jiani Mai Jie Liang XianFu Liu LiuPing Tan Hui Xu YaoHua Li YuShan Zhou ChuanChuan Yang ChenXi Xin |
| author_facet | Jiani Mai Jie Liang XianFu Liu LiuPing Tan Hui Xu YaoHua Li YuShan Zhou ChuanChuan Yang ChenXi Xin |
| author_sort | Jiani Mai |
| collection | DOAJ |
| description | The pharmacodynamic effect of longan leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C1), astragalin (C2), quercetin (C3), luteolin (C4), and kaempferol (C5)) in Dimocarpus longan by ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC). Quercetin (C3) was chosen as the internal reference. Relative correction factors (RCFs, ƒs/i) of the other 4 components were calculated by two correction methods (multipoint correction and slope correction) to effectuate QAMS. At the same time, the difference between the results measured by the QAMS and external standard methods was compared to verify the accuracy of QAMS. Within the linear range, the results showed that all ƒs/i values were obtained with good durability under diverse chromatographic conditions (RSD < 2.28%). The quantitative results of 5 components in the leaves of Dimocarpus longan collected from 10 producing areas by different chromatographic systems and quantitative methods were significantly correlated (Pearson’s r > 97.0%). The applicability and feasibility of the QAMS method established in this study were evaluated to be favorable for quality control of the leaves of Dimocarpus longan. As a new model of quality control, it can provide one more choice of multicomponent quality-control method in the absence of standard substances or instruments. |
| format | Article |
| id | doaj-art-82dbc539c08744a1a7bd9999c2760909 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-82dbc539c08744a1a7bd9999c27609092025-02-03T01:04:23ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732020-01-01202010.1155/2020/39506093950609Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLCJiani Mai0Jie Liang1XianFu Liu2LiuPing Tan3Hui Xu4YaoHua Li5YuShan Zhou6ChuanChuan Yang7ChenXi Xin8College of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaFaculty of Chinese Medicine Science, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaCollege of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530222, Guangxi, ChinaThe pharmacodynamic effect of longan leaves was attributed to various components, especially the flavonoids. In this paper, a new strategy of quantitative analysis of multicomponents by a single marker (QAMS) method was first established to synchronously determine 5 components (ethyl gallate (C1), astragalin (C2), quercetin (C3), luteolin (C4), and kaempferol (C5)) in Dimocarpus longan by ultra-performance liquid chromatography (UPLC) and high-performance liquid chromatography (HPLC). Quercetin (C3) was chosen as the internal reference. Relative correction factors (RCFs, ƒs/i) of the other 4 components were calculated by two correction methods (multipoint correction and slope correction) to effectuate QAMS. At the same time, the difference between the results measured by the QAMS and external standard methods was compared to verify the accuracy of QAMS. Within the linear range, the results showed that all ƒs/i values were obtained with good durability under diverse chromatographic conditions (RSD < 2.28%). The quantitative results of 5 components in the leaves of Dimocarpus longan collected from 10 producing areas by different chromatographic systems and quantitative methods were significantly correlated (Pearson’s r > 97.0%). The applicability and feasibility of the QAMS method established in this study were evaluated to be favorable for quality control of the leaves of Dimocarpus longan. As a new model of quality control, it can provide one more choice of multicomponent quality-control method in the absence of standard substances or instruments.http://dx.doi.org/10.1155/2020/3950609 |
| spellingShingle | Jiani Mai Jie Liang XianFu Liu LiuPing Tan Hui Xu YaoHua Li YuShan Zhou ChuanChuan Yang ChenXi Xin Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC Journal of Analytical Methods in Chemistry |
| title | Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC |
| title_full | Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC |
| title_fullStr | Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC |
| title_full_unstemmed | Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC |
| title_short | Simultaneous Determination of 5 Components in the Leaves of Dimocarpus longan by Quantitative Analysis of Multicomponents by Single Marker (QAMS) Based on UPLC and HPLC |
| title_sort | simultaneous determination of 5 components in the leaves of dimocarpus longan by quantitative analysis of multicomponents by single marker qams based on uplc and hplc |
| url | http://dx.doi.org/10.1155/2020/3950609 |
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