A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells
Background: Increasing evidence shows that <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>) largely affects colorectal cancer (CRC) growth and progression; therefore, the inhibition of intratumoral <i>F. nucleatum</i> may be one realistic approach to combat CR...
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2025-01-01
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| author | Ho Yin Pekkle Lam Meng-Jiun Lai Pin-Chun Wang Wen-Jui Wu Li-Kuang Chen Hsiang-Wei Fan Chun-Chieh Tseng Shih-Yi Peng Kai-Chih Chang |
| author_facet | Ho Yin Pekkle Lam Meng-Jiun Lai Pin-Chun Wang Wen-Jui Wu Li-Kuang Chen Hsiang-Wei Fan Chun-Chieh Tseng Shih-Yi Peng Kai-Chih Chang |
| author_sort | Ho Yin Pekkle Lam |
| collection | DOAJ |
| description | Background: Increasing evidence shows that <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>) largely affects colorectal cancer (CRC) growth and progression; therefore, the inhibition of intratumoral <i>F. nucleatum</i> may be one realistic approach to combat CRC. Although antibiotics are helpful in eliminating bacteria, the major problem remains the rise of potential antibiotic-resistant strains and antibiotic-associated adverse effects. Currently, bacteriophage therapy has gained interest because of its high selectivity to bacterial hosts and may become a realistic approach in treating bacteria-associated cancers. Methods: In this study, a new <i>F. nucleatum</i> bacteriophage, ØTCUFN3, was isolated and its biological characteristics were identified. In vitro and in vivo studies were performed to investigate the effect of ØTCUFN3 in combating <i>F. nucleatum</i>-induced CRC growth. Results: By applying ØTCUFN3 to <i>F. nucleatum</i>-induced CRC cell lines, p53<sup>+/+</sup>, and p53<sup>−/−</sup> isogenic HCT116 cells, our results revealed an inhibition of CRC proliferation and the expression of epithelial-to-mesenchymal transition (EMT) markers. ØTCUFN3 injection also reduced the growth of <i>F. nucleatum</i>-induced mouse xenografts. Conclusions: Our results demonstrated the use of <i>F. nucleatum</i> bacteriophage against CRC, laying the foundation for the future usage of bacteriophage in cancer treatment. |
| format | Article |
| id | doaj-art-82d488bb59714f63a6d19b6dfbe414eb |
| institution | Kabale University |
| issn | 2079-6382 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Antibiotics |
| spelling | doaj-art-82d488bb59714f63a6d19b6dfbe414eb2025-01-24T13:18:42ZengMDPI AGAntibiotics2079-63822025-01-011414510.3390/antibiotics14010045A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer CellsHo Yin Pekkle Lam0Meng-Jiun Lai1Pin-Chun Wang2Wen-Jui Wu3Li-Kuang Chen4Hsiang-Wei Fan5Chun-Chieh Tseng6Shih-Yi Peng7Kai-Chih Chang8Department of Biochemistry, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970374, TaiwanDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970374, TaiwanDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970374, TaiwanInstitute of Medical Science, College of Medicine, Tzu Chi University, Hualien 970374, TaiwanMaster Program in Biomedical Science, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment and Graduate Institute of Public Health, Tzu Chi University, Hualien 970374, TaiwanDepartment of Biochemistry, School of Medicine, Tzu Chi University, Hualien 970374, TaiwanDepartment of Laboratory Medicine and Biotechnology, Tzu Chi University, Hualien 970374, TaiwanBackground: Increasing evidence shows that <i>Fusobacterium nucleatum</i> (<i>F. nucleatum</i>) largely affects colorectal cancer (CRC) growth and progression; therefore, the inhibition of intratumoral <i>F. nucleatum</i> may be one realistic approach to combat CRC. Although antibiotics are helpful in eliminating bacteria, the major problem remains the rise of potential antibiotic-resistant strains and antibiotic-associated adverse effects. Currently, bacteriophage therapy has gained interest because of its high selectivity to bacterial hosts and may become a realistic approach in treating bacteria-associated cancers. Methods: In this study, a new <i>F. nucleatum</i> bacteriophage, ØTCUFN3, was isolated and its biological characteristics were identified. In vitro and in vivo studies were performed to investigate the effect of ØTCUFN3 in combating <i>F. nucleatum</i>-induced CRC growth. Results: By applying ØTCUFN3 to <i>F. nucleatum</i>-induced CRC cell lines, p53<sup>+/+</sup>, and p53<sup>−/−</sup> isogenic HCT116 cells, our results revealed an inhibition of CRC proliferation and the expression of epithelial-to-mesenchymal transition (EMT) markers. ØTCUFN3 injection also reduced the growth of <i>F. nucleatum</i>-induced mouse xenografts. Conclusions: Our results demonstrated the use of <i>F. nucleatum</i> bacteriophage against CRC, laying the foundation for the future usage of bacteriophage in cancer treatment.https://www.mdpi.com/2079-6382/14/1/45<i>Fusobacterium nucleatum</i>colorectal cancerantibiotic resistancebacteriophagephage therapy |
| spellingShingle | Ho Yin Pekkle Lam Meng-Jiun Lai Pin-Chun Wang Wen-Jui Wu Li-Kuang Chen Hsiang-Wei Fan Chun-Chieh Tseng Shih-Yi Peng Kai-Chih Chang A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells Antibiotics <i>Fusobacterium nucleatum</i> colorectal cancer antibiotic resistance bacteriophage phage therapy |
| title | A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells |
| title_full | A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells |
| title_fullStr | A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells |
| title_full_unstemmed | A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells |
| title_short | A Novel Bacteriophage with the Potential to Inhibit <i>Fusobacterium nucleatum</i>-Induced Proliferation of Colorectal Cancer Cells |
| title_sort | novel bacteriophage with the potential to inhibit i fusobacterium nucleatum i induced proliferation of colorectal cancer cells |
| topic | <i>Fusobacterium nucleatum</i> colorectal cancer antibiotic resistance bacteriophage phage therapy |
| url | https://www.mdpi.com/2079-6382/14/1/45 |
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