Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits
The aim of the present study was to investigate the interrelationship of the kinin system, nitric oxide and eicosanoids in the acute phase of antigen-induced arthritis (AIA) in rabbits. The arthritis was induced in immunized rabbits and the following parameters were evaluated 24 hours later: leukocy...
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Wiley
1999-01-01
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| Series: | Mediators of Inflammation |
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| Online Access: | http://dx.doi.org/10.1080/09629359990414 |
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| author | Fernando Alfonso Salamea Palácios Gilberto Santos Novaes Maria Luiza Guzzo Ieda Maria Magalhães Laurindo Suzana Beatriz Verissimo de Mello |
| author_facet | Fernando Alfonso Salamea Palácios Gilberto Santos Novaes Maria Luiza Guzzo Ieda Maria Magalhães Laurindo Suzana Beatriz Verissimo de Mello |
| author_sort | Fernando Alfonso Salamea Palácios |
| collection | DOAJ |
| description | The aim of the present study was to investigate the interrelationship of the kinin system, nitric oxide and eicosanoids in the acute phase of antigen-induced arthritis (AIA) in rabbits. The arthritis was induced in immunized rabbits and the following parameters were evaluated 24 hours later: leukocyte influx (total and differential white cell count), vascular permeability (Evans's blue method), and synovial PMN cell infiltrate. PGE2 and LTB4 (radioimmunoassay) levels were quantified in the synovial fluid. The animals were pre-treated with 20 mg/kg/day during 14 days with L-NAME or D-NAME and/or Enalapril (0.12 mg/ kg/day-14 days), and/or the B2 antagonist of Bradykinin HOE 140 (0.9 mg/kg). Our results showed that L-NAME was effective in the prevention of AIA with reduction of all inflammatory parameters analyzed. Enalapril partially reverted the L-NAME anti-inflammatory effects. The simultaneous treatment with HOE 140 abolished this reversion and returned the inflammatory parameters to the levels observed in L-NAME treated animals. Our results suggest that pressoric alterations induced by L-NAME could not account for all its anti-inflammatory action in this model of experimental arthritis. Additionally the contribution of the kinin system in AIA was characterized as well as its interaction with eicosanoids and nitric oxide. |
| format | Article |
| id | doaj-art-82c2548c3eb04029a979c2f221a872ac |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1999-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-82c2548c3eb04029a979c2f221a872ac2025-02-03T05:58:08ZengWileyMediators of Inflammation0962-93511466-18611999-01-0184-524525110.1080/09629359990414Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in RabbitsFernando Alfonso Salamea Palácios0Gilberto Santos Novaes1Maria Luiza Guzzo2Ieda Maria Magalhães Laurindo3Suzana Beatriz Verissimo de Mello4Rheumatology Division, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, São Paulo, SP CEP – 0124–6903, BrazilRheumatology Division, Department of Medicine, Catholic University of São Paulo, Sorocaba, SP, BrazilRheumatology Division, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, São Paulo, SP CEP – 0124–6903, BrazilRheumatology Division, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, São Paulo, SP CEP – 0124–6903, BrazilRheumatology Division, School of Medicine, University of São Paulo, Av. Dr. Arnaldo, 455, São Paulo, SP CEP – 0124–6903, BrazilThe aim of the present study was to investigate the interrelationship of the kinin system, nitric oxide and eicosanoids in the acute phase of antigen-induced arthritis (AIA) in rabbits. The arthritis was induced in immunized rabbits and the following parameters were evaluated 24 hours later: leukocyte influx (total and differential white cell count), vascular permeability (Evans's blue method), and synovial PMN cell infiltrate. PGE2 and LTB4 (radioimmunoassay) levels were quantified in the synovial fluid. The animals were pre-treated with 20 mg/kg/day during 14 days with L-NAME or D-NAME and/or Enalapril (0.12 mg/ kg/day-14 days), and/or the B2 antagonist of Bradykinin HOE 140 (0.9 mg/kg). Our results showed that L-NAME was effective in the prevention of AIA with reduction of all inflammatory parameters analyzed. Enalapril partially reverted the L-NAME anti-inflammatory effects. The simultaneous treatment with HOE 140 abolished this reversion and returned the inflammatory parameters to the levels observed in L-NAME treated animals. Our results suggest that pressoric alterations induced by L-NAME could not account for all its anti-inflammatory action in this model of experimental arthritis. Additionally the contribution of the kinin system in AIA was characterized as well as its interaction with eicosanoids and nitric oxide.http://dx.doi.org/10.1080/09629359990414Experimental arthritisNitric oxideEicosanoids Bradykinin. |
| spellingShingle | Fernando Alfonso Salamea Palácios Gilberto Santos Novaes Maria Luiza Guzzo Ieda Maria Magalhães Laurindo Suzana Beatriz Verissimo de Mello Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits Mediators of Inflammation Experimental arthritis Nitric oxide Eicosanoids Bradykinin. |
| title | Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits |
| title_full | Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits |
| title_fullStr | Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits |
| title_full_unstemmed | Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits |
| title_short | Interrelationship of the Kinin System, Nitric Oxide and Eicosanoids in the Antigen-Induced Arthritis in Rabbits |
| title_sort | interrelationship of the kinin system nitric oxide and eicosanoids in the antigen induced arthritis in rabbits |
| topic | Experimental arthritis Nitric oxide Eicosanoids Bradykinin. |
| url | http://dx.doi.org/10.1080/09629359990414 |
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