Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota
The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2015/361604 |
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| author | P. Carasi S. M. Racedo C. Jacquot D. E. Romanin M. A. Serradell M. C. Urdaci |
| author_facet | P. Carasi S. M. Racedo C. Jacquot D. E. Romanin M. A. Serradell M. C. Urdaci |
| author_sort | P. Carasi |
| collection | DOAJ |
| description | The evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders. |
| format | Article |
| id | doaj-art-825f6452116a423dba7bf8c67f6a5602 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-825f6452116a423dba7bf8c67f6a56022025-02-03T01:10:24ZengWileyJournal of Immunology Research2314-88612314-71562015-01-01201510.1155/2015/361604361604Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut MicrobiotaP. Carasi0S. M. Racedo1C. Jacquot2D. E. Romanin3M. A. Serradell4M. C. Urdaci5Université de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, FranceUniversité de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, FranceUniversité de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, FranceInstituto de Estudios Inmunológicos y Fisiopatológicos (IIFP), CCT La Plata-CONICET, UNLP, 47 y 115 s/n, 1900 La Plata, ArgentinaCátedra de Microbiología, Departamento de Ciencias Biológicas, Facultad de Ciencias Exactas, Universidad Nacional de La Plata (UNLP), 47 y 115 s/n, 1900 La Plata, ArgentinaUniversité de Bordeaux, UMR 5248, Laboratoire de Microbiologie et Biochimie Appliquée (LBMA), Bordeaux Sciences Agro, 1 Cours du General de Gaulle, 33175 Gradignan, FranceThe evaluation of the impact of probiotics on host health could help to understand how they can be used in the prevention of diseases. On the basis of our previous studies and in vitro assays on PBMC and Caco-2 ccl20:luc reporter system presented in this work, the strain Lactobacillus kefiri CIDCA 8348 was selected and administrated to healthy Swiss mice daily for 21 days. The probiotic treatment increased IgA in feces and reduced expression of proinflammatory mediators in Peyer Patches and mesenteric lymph nodes, where it also increased IL-10. In ileum IL-10, CXCL-1 and mucin 6 genes were upregulated; meanwhile in colon mucin 4 was induced whereas IFN-γ, GM-CSF, and IL-1β genes were downregulated. Moreover, ileum and colon explants showed the anti-inflammatory effect of L. kefiri since the LPS-induced increment of IL-6 and GM-CSF levels in control mice was significantly attenuated in L. kefiri treated mice. Regarding fecal microbiota, DGGE profiles allowed differentiation of experimental groups in two separated clusters. Quantitative PCR analysis of different bacterial groups revealed only significant changes in Lactobacillus population. In conclusion, L. kefiri is a good candidate to be used in gut inflammatory disorders.http://dx.doi.org/10.1155/2015/361604 |
| spellingShingle | P. Carasi S. M. Racedo C. Jacquot D. E. Romanin M. A. Serradell M. C. Urdaci Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota Journal of Immunology Research |
| title | Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota |
| title_full | Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota |
| title_fullStr | Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota |
| title_full_unstemmed | Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota |
| title_short | Impact of Kefir Derived Lactobacillus kefiri on the Mucosal Immune Response and Gut Microbiota |
| title_sort | impact of kefir derived lactobacillus kefiri on the mucosal immune response and gut microbiota |
| url | http://dx.doi.org/10.1155/2015/361604 |
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