A domed window chamber for multi-modality optical imaging
Current dorsal skin flap window chambers with flat glass windows are compatible with optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence microscopy (LSFM) performs best with a cylindrical or spherical sample located between its two 90° objec...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-11-01
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| Series: | BioTechniques |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07366205.2024.2445452 |
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| _version_ | 1832592155729723392 |
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| author | Photini Rice Makenna Aitken Hasina Shir Ricky Cordova Jenna Montague Dustin Tran Urs Utzinger Leilei Peng Ghassan Mouneimne Jennifer Barton |
| author_facet | Photini Rice Makenna Aitken Hasina Shir Ricky Cordova Jenna Montague Dustin Tran Urs Utzinger Leilei Peng Ghassan Mouneimne Jennifer Barton |
| author_sort | Photini Rice |
| collection | DOAJ |
| description | Current dorsal skin flap window chambers with flat glass windows are compatible with optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence microscopy (LSFM) performs best with a cylindrical or spherical sample located between its two 90° objectives and when all sample materials have the same index of refraction (n). A modified window chamber with a domed viewing window made from fluorinated ethylene propylene (FEP), with n similar to water and tissue, was designed. In vitro imaging of collagen gels and microsphere phantoms with and without the dome showed small decreases in signal strength and image resolution due to the dome. Using a custom mouse platform for stabilization and anesthesia support, in vivo multimodality imaging with OCT, MPM, and LSFI was demonstrated. |
| format | Article |
| id | doaj-art-8256946f602d478fba176ec99934ca2d |
| institution | Kabale University |
| issn | 0736-6205 1940-9818 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | BioTechniques |
| spelling | doaj-art-8256946f602d478fba176ec99934ca2d2025-01-21T13:10:55ZengTaylor & Francis GroupBioTechniques0736-62051940-98182024-11-01761153554510.1080/07366205.2024.2445452A domed window chamber for multi-modality optical imagingPhotini Rice0Makenna Aitken1Hasina Shir2Ricky Cordova3Jenna Montague4Dustin Tran5Urs Utzinger6Leilei Peng7Ghassan Mouneimne8Jennifer Barton9Biomedical Engineering, The University of Arizona, Tucson, AZ, USABiomedical Engineering, The University of Arizona, Tucson, AZ, USABiomedical Engineering, The University of Arizona, Tucson, AZ, USABiomedical Engineering, The University of Arizona, Tucson, AZ, USAWyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USAWyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USABiomedical Engineering, The University of Arizona, Tucson, AZ, USAWyant College of Optical Sciences, The University of Arizona, Tucson, AZ, USACellular and Molecular Medicine, The University of Arizona, Tucson, AZ, USABiomedical Engineering, The University of Arizona, Tucson, AZ, USACurrent dorsal skin flap window chambers with flat glass windows are compatible with optical coherence tomography (OCT) and multiphoton microscopy (MPM) imaging. However, light sheet fluorescence microscopy (LSFM) performs best with a cylindrical or spherical sample located between its two 90° objectives and when all sample materials have the same index of refraction (n). A modified window chamber with a domed viewing window made from fluorinated ethylene propylene (FEP), with n similar to water and tissue, was designed. In vitro imaging of collagen gels and microsphere phantoms with and without the dome showed small decreases in signal strength and image resolution due to the dome. Using a custom mouse platform for stabilization and anesthesia support, in vivo multimodality imaging with OCT, MPM, and LSFI was demonstrated.https://www.tandfonline.com/doi/10.1080/07366205.2024.2445452Dorsal skin flapfluorinated ethylene propylenelight sheet fluorescence microscopylight sheet imagingmulti-photon microscopyoptical coherence tomography |
| spellingShingle | Photini Rice Makenna Aitken Hasina Shir Ricky Cordova Jenna Montague Dustin Tran Urs Utzinger Leilei Peng Ghassan Mouneimne Jennifer Barton A domed window chamber for multi-modality optical imaging BioTechniques Dorsal skin flap fluorinated ethylene propylene light sheet fluorescence microscopy light sheet imaging multi-photon microscopy optical coherence tomography |
| title | A domed window chamber for multi-modality optical imaging |
| title_full | A domed window chamber for multi-modality optical imaging |
| title_fullStr | A domed window chamber for multi-modality optical imaging |
| title_full_unstemmed | A domed window chamber for multi-modality optical imaging |
| title_short | A domed window chamber for multi-modality optical imaging |
| title_sort | domed window chamber for multi modality optical imaging |
| topic | Dorsal skin flap fluorinated ethylene propylene light sheet fluorescence microscopy light sheet imaging multi-photon microscopy optical coherence tomography |
| url | https://www.tandfonline.com/doi/10.1080/07366205.2024.2445452 |
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