Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries

A modified direct contact test was used to evaluate the antibacterial properties of four commercially available dental luting agents (RelyX Unicem, Ketac Cem, Ceramir Crown & Bridge and Harvard Cement) and two reference materials (glass-ionomer cement and calcium aluminate cement) compared to a...

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Main Authors: Erik Unosson, Yanling Cai, Xiyuan Jiang, Jesper Lööf, Ken Welch, Håkan Engqvist
Format: Article
Language:English
Published: Wiley 2012-01-01
Series:International Journal of Dentistry
Online Access:http://dx.doi.org/10.1155/2012/529495
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author Erik Unosson
Yanling Cai
Xiyuan Jiang
Jesper Lööf
Ken Welch
Håkan Engqvist
author_facet Erik Unosson
Yanling Cai
Xiyuan Jiang
Jesper Lööf
Ken Welch
Håkan Engqvist
author_sort Erik Unosson
collection DOAJ
description A modified direct contact test was used to evaluate the antibacterial properties of four commercially available dental luting agents (RelyX Unicem, Ketac Cem, Ceramir Crown & Bridge and Harvard Cement) and two reference materials (glass-ionomer cement and calcium aluminate cement) compared to a negative-control material (PMMA). Streptococcus mutans bacteria were placed in direct contact with specimens that had been aged for 10 min, 1 day, and 7 days, in order to test the antibacterial properties of the materials. A metabolic assay containing resazurin was used to quantify the amount of viable bacteria remaining after the direct contact tests. The effects of pH and fluoride on bacteria proliferation were also evaluated. Strongest antibacterial properties were found for calcium aluminate cement, followed by Ceramir Crown & Bridge and RelyX Unicem. Ketac Cem, Harvard Cement, and the reference glass-ionomer cement showed bacteria content either higher than or not significantly different from the PMMA control in all instances. pH levels below 6.3 and above 9.0 were found to have negative effects on bacterial proliferation. No correlation between either acidic materials or fluoride release and antibacterial properties could be seen; rather, basic materials showed stronger antibacterial properties.
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institution Kabale University
issn 1687-8728
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language English
publishDate 2012-01-01
publisher Wiley
record_format Article
series International Journal of Dentistry
spelling doaj-art-8251003597f04afeacd3ff530e3bcccd2025-02-03T06:13:59ZengWileyInternational Journal of Dentistry1687-87281687-87362012-01-01201210.1155/2012/529495529495Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary CariesErik Unosson0Yanling Cai1Xiyuan Jiang2Jesper Lööf3Ken Welch4Håkan Engqvist5Division of Applied Materials Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, SwedenDivision of Nanotechnology and Functional Materials, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, SwedenDivision of Applied Materials Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, SwedenDoxa AB, Axel Johanssons Gata 4-6, 754 51 Uppsala, SwedenDivision of Nanotechnology and Functional Materials, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, SwedenDivision of Applied Materials Science, Department of Engineering Sciences, The Ångström Laboratory, Uppsala University, Box 534, 751 21 Uppsala, SwedenA modified direct contact test was used to evaluate the antibacterial properties of four commercially available dental luting agents (RelyX Unicem, Ketac Cem, Ceramir Crown & Bridge and Harvard Cement) and two reference materials (glass-ionomer cement and calcium aluminate cement) compared to a negative-control material (PMMA). Streptococcus mutans bacteria were placed in direct contact with specimens that had been aged for 10 min, 1 day, and 7 days, in order to test the antibacterial properties of the materials. A metabolic assay containing resazurin was used to quantify the amount of viable bacteria remaining after the direct contact tests. The effects of pH and fluoride on bacteria proliferation were also evaluated. Strongest antibacterial properties were found for calcium aluminate cement, followed by Ceramir Crown & Bridge and RelyX Unicem. Ketac Cem, Harvard Cement, and the reference glass-ionomer cement showed bacteria content either higher than or not significantly different from the PMMA control in all instances. pH levels below 6.3 and above 9.0 were found to have negative effects on bacterial proliferation. No correlation between either acidic materials or fluoride release and antibacterial properties could be seen; rather, basic materials showed stronger antibacterial properties.http://dx.doi.org/10.1155/2012/529495
spellingShingle Erik Unosson
Yanling Cai
Xiyuan Jiang
Jesper Lööf
Ken Welch
Håkan Engqvist
Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
International Journal of Dentistry
title Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
title_full Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
title_fullStr Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
title_full_unstemmed Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
title_short Antibacterial Properties of Dental Luting Agents: Potential to Hinder the Development of Secondary Caries
title_sort antibacterial properties of dental luting agents potential to hinder the development of secondary caries
url http://dx.doi.org/10.1155/2012/529495
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AT xiyuanjiang antibacterialpropertiesofdentallutingagentspotentialtohinderthedevelopmentofsecondarycaries
AT jesperloof antibacterialpropertiesofdentallutingagentspotentialtohinderthedevelopmentofsecondarycaries
AT kenwelch antibacterialpropertiesofdentallutingagentspotentialtohinderthedevelopmentofsecondarycaries
AT hakanengqvist antibacterialpropertiesofdentallutingagentspotentialtohinderthedevelopmentofsecondarycaries